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CRIMIC CR01024

Gene emp

CG2727

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2R:24979379..24979357			Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	ACGAGCCACATAACTCCATCTGGACT	ACGGACACCTGAACGAATGGCTTGAT

Images

Construct Design Sequences

sgRNA sequence	GATTCACAGGTACTCAAAGCTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
ATTGTAGCTCAGCGTGCCAT	GGCGATTTATTTAGCTATCGCA	AGTACCTGTGAATCCAATCGCA	ATGGCCTGCCGACTATTAGC

 

Amplicon 5'

Amplicon 3'

ATTGTAGCTCAGCGTGCCATTGTCATTCTCTACGATGTTCACCTTCTCCCAGGTCTCGCTGCAGATGAGGTAATGGATTGAGCCAGATTGCTTTCATGCTACAAATTATGATCTTTTACCTGTAAACGTAGGGTCCCACCTCATCGACAATGGCCTTGGAGCCGTTGCTGAGGAAGTCGTCCGCATTGGTTACGTTGTAGATGTACAGACTAATTCTGGGCTCCACTGGGGGCTTGGCCCACCATCCGAAGGTCTTGGCGCCGGGACGCAGGGCCACCATTCGGTCCACGACCGCATCAATCAGCACGGTAAATTCACAGGCCACCACAATGCCGCCAATTATCAGCGCCGCTGCGACGACAATCGTAATCCCTGGAAAACCAATGCGAATTGTTTAGCAATTGTTCACCGATTGCATAAATTACCGGATAAGTGGATTGAGTCATTTGATATTAGGTGGGGAAACGTAAACTGCGTATTGCCTTTAATCAGAACTCATATTTGAGTTGGCATTCGACCGAAAACGCTCGATTGCAATGTTTGGGATGGGAAATCAATATTTATAGATCGCCGATCGAGCTTTAGGAATGATTCACTGACCCTTTGACCTTTAAATCCCAGTTCTACAATATCCCATTATCTATAAATACGGCCAATTAGTATTGATGGAATAACTATACACCAGAACTGGAAAGTCTAGAGAACTTCGGAAATAAGAAAATTGCGGAAAAGGGAATGCTCGGTGAAATTGAAAGTCATCTTATACCTATGTATGTTCTCTGTAATAAATAATATCATTTTTGCTCAGATACCAGTCCAACCAGTCGAGTAAACAGGAACTATAATTTCGGTTTAAAACAAGAACGTGGCACGTGGATTTATGACACCTATGCGATAGCTAAATAAATCGCC

AGTACCTGTGAATCCAATCGCAATGCGTTGTGGATAAGTTGTTTGATTGGTTTATTACGATACCAATCTGGAAATTTATATGGTAATTTCTTGCATGGGAATGCTGCCAGCAATGGTGTGGGTGATTTGGAATTAACCTCCAGGTCTTATCTAAATGGAATACTTACACCATTTTCGCAGATAGGTGCTGGAGAGTTTTGTGCAAAACTTGTGTTTCGGCCCGTTCATGTTGACCAAGTCGGTTTTGGGGTTGGGTGTGGTGGAGAGTGAGGAGCAATCCGCCTTGCAGTTGTTAACTAAAATGCAAAATAAAATTATACATAAGGCATATCGCGTTGTTTTACAGAGACCTAGAGATACACGGACACACTAGCCGTAATACCGGTTAAATTGTAGTCTCAGCTCGTTAATCTATAATTGCCAGTGGCAGCCAGACAAACAGGAATTCAATACAGCATCTTGATAACTGCGTTCTGAATGACATTGGGCCTAGGCGGCCATCGCTTCGCGATCATGACTCTCAGATTGCGATCAATCTTTTCGTTTTGCCCAGTCTTTCCATATGCAACTGCCGTATTTTAACGAGAATCCGAGGCCCAAAGACCCAAGACACGTTTTGGGGCGTGTTAGCCTTGTGCTGTTTTGACATCGCTTTGTCAATATGCGGGCTTTTGGCCGAAATCGTATTAACAGCAGCACAAAAGCCAACAAAACCAATCCGGCAGTCCAACCCAACGGCGCTCTGACTCCAGAACCTTCGAGTCAGCGAACTTTGTTCCAGCAGTCGAGTGCGCCCTTCCTGACAGAGTCGGACCTTGCGCCATTAAGCGATTTTTAATGGGCCAACAGGCTCGGTCTGCGCTGAGGTCAACCTGTGAGCTACCTTGACCGAGGCCAGGGCTGCGCAAGCGCAAAACACATGAGATACTTGTATTTTTCGTTCGTAAACCGAGACATTTTTATTATTGACAATTCCAGCCACAGCGCTGTTCGCCATTATTCATATTTATAAGCGAAGCCGAAAAAACCATGCAAATGAAGCGACATGATGCCAAATGGATTTCGGCTAATAGTCGGCAGGCCAT

 

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