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CRIMIC CR01026

Gene Spn43Ab

CG1865

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2R:7153129..7153151	42F3		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GCTCGAGGAGGTGAGTTAACAACTGT	TTGGCCTTGTGCTCCACTGCGTTAAT

Images

Construct Design Sequences

sgRNA sequence	ACTGTCGCGAGGAGAACTAAAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TCCAAACCCAAGCCAAAGGT	ACAGTGTCGCAGTCCAAGAC	GAATCGATGCGAAACATGGCA	TCCAGAGTGGTCTCCTGGAG

 

Amplicon 5'

Amplicon 3'

TCCAAACCCAAGCCAAAGGTGACACTAAACTTGGGCAGCAGCACCTCCACCTTTTGCTGGCTCATCGCTCCGATTTCGGCCAGAAGATTCTTACCGGACAGCGACTGCTGCAGGGATCTCAGACCATCCTTGCGATTGGGCAGGAGCAGCAGCATGGAGAAGTCGGGGTTCTGGTAGGGCAGCTCCACCACCTTGGCGTCCAGGGAGTTGACCTCGGCGTAGTTGAAGTTCTGCAGCGTCCACATGGTGTCTACCTTGACAGACTGCCCGCTTCCGGTGCGGAAGGAGCGCTTTTCGGTCTTGTCCAGCCGGAAGGCCTTTTGCCAGGCGGCTGAGTAGGAAACGCCGTTCACGATCACTCCCTCGGTGCGATCATTCAGCAGCTCGGCGCGGAGGATGTCAGTGATCTTGCCGTTGGTCTTGGTGGCCACCGCCCGGTTGATGCCATCCGCCGTGCGCTTGTTGTACGGCGGGTGAAAGTCCAGCTTGTCGATGTTCGAGTCGAACTGGCGCTGGGCCACGTCCCTGAAGGAGCCCTTGAACTCAAGGTTCTCGTTGATGTAAATGTTGTTGGCCAGCCGGAAGTTGTTGTCGCGGGTCAGGGCCTGTTGGTAACTACCGCTGCGCTGGCTCACCGCATCCGCATCGCCAGGACCTAAACGCAGCCCCTGCTGCAACTCCGATGCCGTCTGACCCTTGGCCCCCACGAAGGCCAGCGCCATGGAACTCTGTATGGTCGCTGGCGAGATGACCACATTTTCGTTCAGATGATCGGCGGCCACGGCGTTGTAGAGATCGGCAGCCAGCAAATTGCGATCGGCAGCCGCATCGTAACCCACGGTCTTGGACTGCGACACTGT

GAATCGATGCGAAACATGGCATTAGTCGCAATTTGTTTGCAGGCTCCGCCCCGCCAGATGAGGCACACTTGGAGAAAATTATAGCTCGAATAATTGTCACATTTAATTCACATATTTTTTCGTGCGATTTGATTCACTTTTTATATAATTTTCACAGAAATAGGAAGGATATTTGTCACTTTGAAATCGATTTGACTTTATCACCAAGTTAGAAACTGTGGCATTACGTCAAATATCAATTGAAAATAGAAGAAATTTATTCTTAATGATAATAAAACACCCAAAATTGTAGCAACTCTTTTGATAGCAACGGCCTTTAAAGTGGAAATACATTCCTGTACAGCTTTCGTACAGTGCACAAACCAGATCGCTTAATTGGGGCTTACGTAATAGGCAGCTGATGATGACAGCCATGGTCGTCACATAAATTGTTGATGGGCGTCTCCTGCTCCGCTTGATTCGCACTTGAGCTCGTCGGAATGTGAACTGAAATCTCTGCGATCGCAGAGCGCCTATAGAGGTTGATCCAAGTCGGATTCAAACACGAAACTCAGCTCTCGGGTCCCAAATACAAATACCGATCAAGTGTGTTTACAAATCATTTGTCACCTTTGGCTGGCCACGGGTGATTCTGTTTATTTCGCGGAGATTTGAGTGGGGCTGATAAGGCGATAGCCCGCCCGGCTTGAACTAGCATTGGAATCGAGAATTAGGTATGCCTAGAAAGACTCAGATGTCGACGATGTGGCCGGACAGCAGCAGAGTTTTGCCATTGCCGATGGCGTAAAAGAACGGGTGATTGATGACCAGGGGCAGGGCTCGTCGGAACAGATCCCCGAAGGCTAGAAGTACACCTGGTTTAGGAGGCTTCTTGGTCAGAGACTGGCGTGATCACTTACAGAACGAGTCGTCGGCATTGCCGCCATCCTCCTGTAGCTCCAGGAGACCACTCTGGA

 

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