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CRIMIC CR01028

Gene CG44247

CG44247

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2R:24878175..24878153	60E5		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AAAATCCTCGAGAAGTGCAGTTGGCG	TTCTCCATGGCGATGATCTGCTGCTT

Images

Construct Design Sequences

sgRNA sequence	TCCATCACAGGCGGCTGAATGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GCAAGTGGGAGTGCATTTGG	TCCTGCAAGAATTCGGATTCGA	GATCCGCAGAGTGAGCAAGA	ACAGGCATTGGGAGCTTCTC

 

Amplicon 5'

Amplicon 3'

GCAAGTGGGAGTGCATTTGGTGGCAAGTGCAAGATGGCGGCTCGCCTGGAAATCACCGTTAGCTTGGCGCTCGCTGCAACGCTACTGTGCAGCATATTGGGTCATTGCGAAATGTCCGTTTACCCAGGTAAGCCTAAAAATAGCGCACTACACACCCATTCACCTATAAGCCCGTACACCCATGTACCCACACACGCAGCCTGAGTGTTCGGGCTTGAATGGCGCAGGAGGAGCGAAAGGAAAGGAAAGGAAAGCTGCCACCTCGATTTGTGTATCCCCTGCACTAGATACATGCATGGCGAGTCCTGCGAAGTGCTCCGAATCAAACCAAAACACCCACGCTCATTGCTTCGGCAAAACTAAAAAGTCAAGCCAACTGCAGCGTCGTAACTACATAAATACACATAAGTGGTAATAACGAAATTCTCCACTCGAAATTTACATTCATATGTATGCATTTGGTATAAATATATGTAATATGGATTAGCTACGATTTTCGTAGCATACATTTAAGCGCCTTAAAGCTTTTCCGTTCATCCCAACAGCTATAACAAATACTTCCTTGATACAAGCGCAACAATGGGCTGAACACGACGAAAACAACAAAGTGCGATGGAATTGCCACGCACGTCACAGACTTCGGTTCCCAGTCCTCCGACTGTATGTTGGTCTCCCTCACTCTGCGGGAATTGCTCGGGTTGACCTGGGCGCGTGTGTAATTGGTCTATTTTTAGCCTCTGGCAATAAAACGAACTTTTGTTTGTCTATTTCGAATCCTTTAAGGCTGTAGGAGGCAGACCACCGCATTTCGGCCATTCCATCTATTTCTATTTCTATTTCGACTTCGATTTCACTTTCTATTTTCCGATCCCTAACACGCAGCCCAGGCTCATTACCATTACCATAATCATCTGCCCGTGCCCACTAATCCGATGACATTGATACGATTCGAATCCGAATTCTTGCAGGA

GATCCGCAGAGTGAGCAAGAGTATGCTTTTCTGGCCCAGATGATGGAGCAGTATGCCCGCCGTCGGCTGCAGCAGCAGTTCGACCAACAGCTGCACGAAATCAACCTGAAGATGGATCGTCAGCGGCAGCTCTTGGAGCAGGAGCGGTACCGCCAGCGGCAGCAGGAGCTCCAGGTACAGCGCGAGGCGGAGGAGGACTACGAGTCCAACGGAAACAACCTGAACCAGCAGTACGACCAGTACGACAACGCGGAGGCAGAGGCCAGCTACGGCAGTCCGATGGAGGCAGTCTCTGATCTCCAAAGTCCTCCATTGTACCTGCCTCGCCTGCCCAACCTACCACCCTTGCCCAGCCTTCCGAATCGGCCCAGCCTGGGCAACTCCCCGAACAGGAACAGTGTGCTCCGCGAACGGATTCCCTTTGCTGTAGGTCCCAGCGACGCTCGCTTCTTTGACAACCCGAACGACCCTTCGGGGGAGCTGGATTCCCGGGCGCTGGAGGACTACGAGGAGTATGCTCCCATTGAGCCCACAACCGAAGTAGCCACCACCGCCAGTAAGACCAAGATCGATACGCCAGTACCGGCTCCCGTGCCCGTTGAGCCGCCCAAACTGCTTGACGCCGCCAACACCAACTTCCACCGCATCAATCCCCAGTCCGCCGCCGCCGCAGCCGTGGCGGGAGTGAATATTCCCCAGAGCAAAGAACAACCACCCCATGAACTTAACGCACTGATCAACAAGCTGCAAAAGCAAAGCAAAGCGCCCTCTCATTTGACGCTGGTCAACGGAGGTGATTCCAGTCAGGAGCAGATTGTGCTGCGCCAGCACCTGGGCATGAACAGCGAGATGGGGGTGTACATAGTGGCCCTAATAGCGGGGGTTAGTGCAGCGGTGACCGTTGGGCTTCTTGCCCTCGGAGTAACTTGGTAAGTAACCCAGAGAAGCTCCCAATGCCTGT

 

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