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CRIMIC CR01042

Gene CG31935

CG31935

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	2L:1596841..1596858	22A3	FBti0206881	83251

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GCGGCTCTGGACTTAAGGTCACATAA	CCAGTTCGGCATTGCTTAGAATGTCC

Images

Construct Design Sequences

sgRNA sequence	ACATCAGGGCAAACTATGGGAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GAGCCTGTTCCCAGACCTTC	TGATGTATGCGTGGCGACTT	GGTCCCACGTACAGACACAT	TTTTCGGGTCGGATTGGTGT

 

Amplicon 5'

Amplicon 3'

GAGCCTGTTCCCAGACCTTCTGCCAGGTGTTGCCCTCAGTGGTCATCCGTATACTAAGTTGATGCTTCACCTGGCCCAGTTCTTTAATAAAATAAGGTATAAATATGGAGAAATGATTCATCCTTATCAAATGAAAATCACGTACCATCAGTCACCTCCTCCCAGTCTTTGGGGCTATACCAGCGTATAAAGTCCTCCATGATGCCCCTGGGATTGGCGGCCTTGAAGGCTTCCATATCGGAGAGCAATGAAGTACACATCATTTGGGTGGTCAGGCCCGAACCGGGTCCAAGTTTCAGCATAACTTCTGCGTCGTCCTGCAGCTGATCTTCGGTCTTTGGAACGGGCTCCTGGGTATCTGGGATATACAGATACTCATCGGGCTCCTCCAGCAATCGTTCGTTGTTCAAACGCCTCAGGCGACCCTCGGGCTTCAGACTAAGAACTGCTTTTGTGGGTGAGCCCGCACCGGCAGTCAGATCATCGCAGTCAAAGAACTCGCCCTCGTCGTCATCCTCATCCTCTTCCTCCTCGGAACTAGCTTTTCCCACCATTCCCTCGGATTTTCTTTTGCTATTCGCCTCCCGTTGCACGCGACGCTCCACGCAAACGTTAAGCATCTGTAGTTTCTGGTGGAGGAGGCAGGTGCGCGTGTCGGGAAAGCCAGCGGAAATTCTGGGGGAAATCCAGTAAATTCGAAGGTGTTAGCATGTTGAGAACAACAGCGGAGGTGTTGATACATTCACTTAATTAATTAGCTTTATGCCGTTTGGTTTCGTTTATTTGCCTTATTGGATTCTTTCCTCTTTAGATATTTAACAAAACTTCTTCTGTGATTGCTTTAATACGAAATAAGGTGGTTGTGCAGCATTTATAACCTTAACTTTGCTTCTTCCTCGAGTCACAGTGTTCAAGTTTAAGATTTCATCTAAGTCTTAATTTATGCTCCAACCTTTTCATGGCTGGGCCAAATCTTCCACCGCCTTGTGGGCATATCAGTCACATTCTTCGGGCCAATCTCAGTCGCCGTGACAATAAACCCGACAAAGGCGGCCAAAAGCGAGCTCTCTGGGCCAAGTCGCCACGCATACATCA

GGTCCCACGTACAGACACATACATGTATCTATCCCCTTCTGGTAAACCAATTGATATTCACGCTCATCATGGCTGTCAACGCGTGCGTGCCCCTCTGGTCTTGCATAGCCAATGAGCCCCAGAAGTGGAGGTGACTCAGTCCACAGAGTTCGGTGAACTTTTGCAGCAGAGTAGGTTTTGGGCCCGGCTACAGGGGCCCCCTTAATGTAGGGGGGAAGTGAGTTATTTGGACCAGGACAGTGACTGCCTGTTGGGCAACCGAAAAAGGGGAGCCCTGCAGCTCCTGCACTTGGCATTTGCATAAAAGTTGCCTTATTAGTGGCTGCGTGGACAGCTGCAGCGCAGGAGCAGCTGCTTAAAAGGTTTTTAAAGTGGCTAAAATTGCACTCAACTTGGCAGCGGGCGACCGAGCAAGCTAGCTGACAAATGGCTCTTGGCTTTTATTGACCCAATGTTGGCCACTTGACAATGCCCCGCTCTCTAGGCATTTGGCCACCTCCTCGACACGCTTATTTTGGCTGCAGTGAAATAGGAACATCAAGTGTGCTCCAAGACATTAATTCTATGTCTATTTTTACCGGGTTCCCCACCATCATCATCGTCATGGGGGCTGCCCCAATAAACTAGCTCGAGTTACGTTTATTTTTGTGTGGCCAACTGAACAATTCAGTCGGCTTCAATGGCCAAAAGGATAAGCTGCGGTTAAATGGCAATTGTTTTTTGGCCTCAGAGCCAAAATGGAATACTAAAGCCGGAATATTTAAAAGGGGATATTGTCAGGGGATTTTCGAGGAATTTATGGTATAATTCAAGCGCTCAGAAGTGCGACAACTTAACTGTATCAATTATTTAATAATTTAAAGTGATTTATTATCCTAAACAAATTAGTTGAACTCAAAGCTCTGAGACCTTTTCAAAGGATTCCGACCCTTTTAACGCCGCGGCGAAAGTTAATTCTCCCTTGCAAATGCAAAATTAATATGCCCCGAAACGGCTTCAAACTTCATCGGTTCAAGCTCAAAAAGCAACCCAGTTCATGTGGCTGCTCTCGACTTAATTCCTGTGGCCAAGCTGACCCAAAAAATTATCGGCCCACGCACTCACACCTGTCCACCTGTCCACCACACCAATCCGACCCGAAAA

 

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