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CRIMIC CR01046

Gene Gyc32E

CG33114

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2L:11144344..11144348	32D5	FBti0202664	81160

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CGAACTTTTGTGTGGTATCCCCATGG	CCATGGCAATGTGCTCACCAATGAAG

Images

Construct Design Sequences

sgRNA sequence	CGTGGGGAGAACTTCCCGAGTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TACATCCGAGCCCCAAATCG	GAGTGGCAGGATCTGGTGTT	AAGTTCTCCCCACGCCAAAT	ATGGGTGCCATGTTCTACGG

 

Amplicon 5'

Amplicon 3'

TACATCCGAGCCCCAAATCGGTTTCGAGCTGACTACTATGGAGAATTTATTCCAATGGAAGGCGTTCAGCAGACTAATAACACTTTTCGAGACCTGTGAACATGTAACTTCTTTTAGTAATAATCCATTTAGCTGGGTTTTAATCAGAGAGTTACCTTGGAAGCCGGCGCTAAAGTTCGAGCAAAGGTGTGAAATGTACTTTTGTTTGAGACAATTGGATCGGAGCATTTCTAGGGAATATAAAAAAGTTACTATTGTATACATGTGCAGCAAGTCTCAAAAAAGAAATTCAAACTGTGTCCTAAATAACAAATGTAATTTGTGCAACTGGGCTCTAAAGTTGTGTAGTGTATTTTGTATACAAGAAAATAAATTTATATGGCTTTTGGTTTGTTGCACTTTTTTTTATGACAATTGGCAAATAAATTGTGAATCAATCTTCCAAGCAAGAGCATTCTTCGTAAACTGTGACAAAATAGTCTTTCATAAGTAAAGGGCAAAGGGTGTAACTCTTGAATTGAACACCGGTGACCCAGATTTAAGTAATGGGTCTACTAACGAAGGATAGCATTGGTGTGTTCCATGCGGAGGCCAGCAGCGCCTCCGTGGTGCAGCTCTCGTCGGGACCAATGAATGCGGTCACGCCAGCTTCCCGCATCTGTGTCATCGCCCTGGAAACGGAAACGGAAACAGAAGCGGCAACGTCAACGGCAACCGGAAAAGAGCAAAATTACAGATGGCAACTGATACAGGAGTTATTTAACGTAATAGTAGCATCCGAATGAATTACCTCAACGGTTTAACGCGATAGGCGCTCATCTTGTGACCAATGTCAACCGGTTTGAAAGCCAACTTCTTGCCCGGCAATAGGTTGGGATTCTTGTTCACATCCTCGATGGCAAGTGGCAGTCCGCCGAGTGTCACCTAATGTAAATAGGGCCAATTAAAGTCAGCGATGAGCAGTGAATAATAACACCAGATCCTGCCACTC

AAGTTCTCCCCACGCCAAATGGCGCTTAATTTATTCATGTTGTGCCAGTTGTGGAGGACCAACGTAAAGCATAACAAAGCAATGCAAAACAGTATTATGTGTACTTGGTTTCACCCGAAGGCTGTGTTAAAAAATAAAGCAATTTTTATAGAAATCACCAGAAGTTCCAGGATGTTAAATTACATTTCCTTTCCTTCGGTCAGTTCAATTCAAAATGCTTAAACTAAACTTGATTAAAATTTCGTTTACTGCATTTATAAAATGTACACGATAACATTGCTTCTATCAAATAAAAACAATTTCCAATTTGTTATGTTCTAATACTGTCCCATACAATAGAGGTTTAATTTATGGAAAATGCTTGTAGGTACCCCAAACCTCTCATTCGGTGACCATTATCATCTCAAATCCGAGAAGTTCATTGATTCCCCGTACATAGGCCCGCAGCTCGTGGTCTCTGCTGTGAATGTGCCTGTCAGCCAGCATCTGAAGATTGAGTCTCACAACAGAGACCAAGCTAGTGATGGCCAGGATTAGCAGGTATATCCACAGTACAAAAGCCAGAATGACGTGCTGCAGACAATTGTCGGTCTCTAAGCACACCTCATTCGCAAGAAACTCCCACAAATGGCAAGGGAATAGGCTTAGGCCCTGGGCCACCTTCAAGGTCCAGGGCACAAGCATCACGGGACTACATTGTTGCCACACGGACAAGCCAGCTGTTCGCAGCCTGGAGAAGAAGCACCCGAGGGGTCCTTTGCTGGGCAGGTGTATCAAACCATAGCCGTAGAACATGGCACCCAT

 

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