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CRIMIC CR01047

Gene nolo

CG31619

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2L:21700695..21700673	39F1		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK (weak)	TCGTTCGCACTTTCCACTAGCTGAAC	CTCGCAGACAATTGCACAATGACGCT

Images

Construct Design Sequences

sgRNA sequence	TGTATCCAACAGGCATAGGGTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GCCAGCTTACAGTTCGAGGT	TTAAGGAAACGGGGGAAGCA	TGGATACACAGGCCGCCT	GCGCTTGCGAGAAGAAAGAC

 

Amplicon 5'

Amplicon 3'

GCCAGCTTACAGTTCGAGGTCAAAGCGAAGCCTTATTAATTCTTTCGTCGAGACAGTCTCCATATAAGGCCGGAAACAGTTAACAAAGGCTTAGTCCTTGGTCACTTACAGTCGTTAAACGGTATTAGAAAAAAAGACATTCAAAAAGACAGACAGACGCAATGCGTAAGTTTCGGTTTTAACCTAAGCCTGATGTTTTTTACTTTTTAAGGGGATGTGGGCTATGACAATTGACCTTTGCCATATGCGTTAAGTGTTCCAGTCTGAAATGAATGGCAGAGTAACGTCAGTCAAATGGATAGACAAGCCGAGACAATCGGGCAGGTTAGACATGGCTCAAAGTCAAAAAACAGATCGCAGTGGCACAATGCCATAGCAGAACCATCAATTTTGAAACCCAAAATCCCAGAACTCAGTTCATCCATCTATCCCTTGTCAAGTCATCGTTTTGGAAGCTGCCGGCTGACTGTTGTGGCTATAGCCACACAGGCTCGACATCGCCCTAGCCATTGTATAAGCCAAACAACCCTAAAGGGACGTACCTTGAATCATCCTTATGTTTGTGGCCCCAGCCTTTGCAACTTCGCACACGAAAGTTTTAGATCATTGACGTTTTGCGCCGCCGGTCGCTGTCGCTTTGAATAAATAACTGGCGTGACGTTATATACACACGTACTAACGGGTTTCTTACCCCCGTCATCTTTGACATTCTAACCGTCTGTTGGATTGTCCCTGTTCAATCCTGACGTTGTTAACCATGACCAAGTATTGTTTAAAATTTGCCCGACTTCTACGTCACCTTTCCGTAATGTAGTACCTCTGATTGCTGTTGGAAATAGACAAGGCTTTATATTCTGGTTTGAAAACTACCTGGCATGTTGGGTAATATAACAAAACGGAACAAATTATCTCGACTATCTTATACCCATCATCTCTTTGATAATATAAGGATAGTTCAATTTTCTAAAATTAATATTTAAGGATGGCGTTCGAGACCTAGAGTAATTCAAGTTGTATTATTATGTAATATATACCCTTTACTGTGTGCTAATTACTAATGCTTCCCCCGTTTCCTTAA

TGGATACACAGGCCGCCTGGGCAAAAAAGCTAAATGCAAGTCAAGCTTTTGACGACACGTGGAACACAGCATCCGATTTGGAAAATGAACTGGAAGTAAGTAGTTTTGGCTTTTCTGTTAACACTGCACAGAACATTAAATCCACAAATGCAACTGCTCATTTTCAGCAACAAAAGCGATCGAAGGTCCAAAGTGCAGGAGGAGGAGGTGGCGCGGGCGGTGGACCTGGCCAGTGGTCGTCCTGGTCAGATTGGTCAACCTGTTCGCGCACCTGCGACGGAGGCATCATGCATCAAATGCGTAGGTGCGGCAGTCCTGGCAGTTGTCGCGGGGAGAGCACCCGCTACCGCATTTGCAACATGCAGCCTTGTCCGGAGCAGCAGGACTTCCGCTCCAGCCAATGCTCCGCCTACAACGATGTTCCATACGATGGCACCCTGTACAAGTGGACTCCGCATTATGATTACGTCGAGCCCTGCGCGCTTACATGCAGGTGGGTGTGCGTTGTGCGTTGCTTATAAAAATGGTTTGCTATGGAGTTCTAAAATCTTCAGTCAGCTCAAACACTTTGCCGCCCATTAAGCTTAAGTCTCCAATGATTCAAACAGAGTCGGTGCTGGCATGTTAGTTATGGCTAAACTATACTTTAAGCTATTTGCTTGCATTTTGATGAATAAACCGAAAGTTTGCAATTAAAAATAATGGGAGAATTAACAAAAATTTGGGAATTGTCTCTTCCAATCGACAATGTTTATTCGGTCTTATGGTCTACGCCTTATTACATTGCTACATTAAGTGACTACAATATAAACTGACTATTTTATTTATTCAAATATTATATATAGTATTTTAAAAGTATTTAAATAATTACGAATTTTTTTAACACAACTGTTTTGATATTGGTGGGCATGCCAGAAACTAGTATAGAGAGTACAAAACTTCAAAAATGCAAATAAACATAATGGAAAATTCCAACGGATTATTATTCCCTGATTATTATTAGTCTTTCTTCTCGCAAGCGC

 

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