• About
• Search
• MiMIC
  • Technique
  • Constructs
  • RMCE Lines
• Transposons
  • GDP
  • Others
• CRIMIC
  • Technique
  • Constructs
• Order
• Downloads

CRIMIC CR01048

Gene CG31871

CG31871

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2L:10667814..10667846	32A3	FBti0210322	Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
short band	OK	GCGCACATTGTTTGCCCGGCAATTTA	AATCCAAAAACCCGAAGCACCACCCA

Images

Construct Design Sequences

sgRNA sequence	AGGCACAAGCCTGAGAGGATAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
AACATTTGTCGTGTGGTGCG	GCCTGGATGTTAACCCTTCCA	ACCACAACAGCTTGTCTGGT	CTCGTGGAAGGTGAAGTCCC

 

Amplicon 5'

Amplicon 3'

AACATTTGTCGTGTGGTGCGAGTTTCAAAACATCCCGAGATTGGCCAACACGCTGGGCGGGAAAATCCAGCTGGCGGCAGCACTTTGTTGCATTTTGAGCATCTTGCAACGCCTTCGCGTCGCTTCGCAAATATTGGCCGGCTGTTTGGCCAAATGTGTGTCAAGCTAAGAAAAACAAAAACTTTGCCGGGTAGCATCGCCAGCAGCTGTTGCAATTTTTTCGGGGCTTGGCCAAGTCACTTTCTGCGGCTTCTCTGCGCCCCGTTCGCACGTTCATTCATTCTCGAAACAAAGAAATTACGTAAGACAAAAGGCAAGGAAATTGACGAAAAAAACACAAAAATAAAGCGAAAAAAAAAACAAATAAACCAAATTTATGCTTAAACATTTGGCAAAAATCGGCCGAATACAAAACATGCAGAAACGGAGCAGGAAAAAAACATTTCGCTGGCTTACTTTGCGTACCACTTCCTCATGTTGTCCAACCAGCCGGCAAGTCAATTGGCCAATTGCAGTGTTTATATTATTTTCTGGCCAACAACAGGAGGGTCTATTCGCTGCCAAGGTTAATTAAAATGCCAGTCGGCCCGCAGAGCCTCTCTTTCTCTAGCTTTTTCACTCATTCCAGCTGATTTTCAGTTTGTTTGCTTCGAAATTTGGGTAATCGCGTGTTTATAACTTAATTTCAACAATGGTGTTATGAGTTTTTCTTATGGCCACCCACTACCCGACCGGCTTACATAATCGCACAGACTTATCAAACGTATCACGGACTTCTGAAAAACCTATAAATTCATTTGCCAGCAGGAAGAAATGTATGCTGTCATACAGTGCGTATGCGTGATACGTGACTGGGTTTAACTATGGCGATGTGCTTATAGAGGTTGCCGCGTGTATTTTGCCTCACCTGTAGTTCCAATAAAGCATCGCCAATGTTTTGCTTTATAGTTAGCCCGATACTTTTGTTTTCATCGGTGTCAATAAAGCGGTTCTATAAGGTTCTGTAAGCCAATATAGTGGCTAGAATGTCGTTTTACCTGTCTGCCTCTGAAATAATCTACCACAACTATTTCACTTAGCTTTAACTTCTCTAAGATGGAAGGGTTAACATCCAGGC

ACCACAACAGCTTGTCTGGTAACTTTATTAACTTGTTGATGCTGCAAATTAAAATTCATTACTTCCAAATGACTGTGGTTAAGGCCAGGCGATTGCCTCCTTGATGACGATAACGCTGCCAGTGGATACCGGTGGTCTCCTCCACCTGCCCTTCCATTTGAACTATGCAAATTAACGGGGCAAAAACAAAAAGCAGTATGGGCAGGTGGAACGTAGAGCATGGCAACTCAATTTCACACTACCATGATTTGCACTTCTAACACCTTTCTCTCAATCTGACGCATTTTTTTCCTCCATTTTTTCGCTCTTCCAGCCCAAATTAATACGCAAATATGGATATCCATCGGAAACCCACACGGTGGTGACGAAGGATGGCTATATCCTGGAAATGCATCGCATTCCCAAGAAGGGAGCCCAGCCGGTTCTCCTCATGCACGGAATTCTTGATACCTCTGCAACATGGGTGCTTATGGGACCCAAGTCAGGATTAGGTAAGCTATTTTACAGGGTTAAAATATTTACTTTTACTCAAAAAAAAAGGACAATATCGTTTGGCTCAAAAGTGATTTTATTTTAACTAAATTTTTGGCTTTAAAAAACTGTTCTCAAATGAGTAAGCAATTTGAATACTAACTAAAAGAAAACATTTAGCGATAACTTCATCTAAAATAAAATATTATCAACGTAAGATTGAATTATTTAAATATATATATACTTCGCATCGATGGTTTTTGCAATTTTCAGTACTCCTTTTGTATCATATGCAGAGGTATTTAATAAAATTTCCAAAATCTATATTGAGTACCTGCCTGTGATGTTAATTAATCAAATTAAATATGTTATCCAGGCTACATGCTCTCCGATCTGGGCTACGACGTGTGGATGGGCAACTCTCGGGGCAATCGGTACTCCAAGAACCACACCAGTTTGAATAGCGACTATCAGGAGTTCTGGGACTTCACCTTCCACGAG

 

---

Search Again