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CRIMIC CR01049

Gene dpy

CG33196

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2L:4573463..4573485	25A1		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	short band	CCAAAGGTGTTCGTTCGGGCATTACT	TTTTCTGTGCCATTGTTTGTGGGCGC

Images

Construct Design Sequences

sgRNA sequence	CGGAGTTCCCTCATTCACACCGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CCATGTGGCTGTGGCTTTTC	GGAACTCCGAGGGGCTTTAA	CCTCTCGCCGACTGCATAAA	GTTCGAGATGACCAGGCCAA

 

Amplicon 5'

Amplicon 3'

CCATGTGGCTGTGGCTTTTCAATTGCTTTGTACCCACTTCACTTCAATTCACTTCACTTCACGCCATGCAACGCTAACGGCCTTGCTAAACAATAGATTCTGCCGACTCTGCGACTTTTATCAATTGACAGGCGGCACAATGAGCACCACATGCACTCAACTGAGGCAGCAATACTCGCAGTATGCTGCAACAATTAAAGGCGTGCGAGCGAAGATGTGCGCAGTCGATGGGGATGCTGATGCTACACACTGATCCAACTGATGCCACCTCTGATGCCTACTGAAAATGACGATGATGATGATGATGATGCGACTGCTGAACGCCAAAAGCTCCCATGACATTGAGCGGCATATGCCAGCGACTACAATGCGCAATTTTTCGTTGCCAGGCTTGTTAACAGGCGCAATGACAACGGCAGACAGATGACGACGTTGCACGTCAACGCTCAAGTGCACATCCGTTATAACTGTCAATGGGTATTAACTTGGCTGTGTTTGGAAAAGAAAATCCAAGCTTCTGCTATATATTTACTTTTTCATTTTAACACAATCCTAACTGAACTGAATTTTTAATGAGAATTTACAAACATTTTGTTGTTTATAAATAGGGAGTGGTTTTAAGTTCGCACAAAACATTTGCAAATATTGTCCTCACATCAACAAAATAGCCGTCAACCGGCAAGTTCGTTGCCTGAGTCTTTTGTTTGCAAAAAACAGCATTCTCGAAGTTCACAATTAATTATGATCCCATCTAGCTAATGAAATGAATTATGACTGCCTCGCAGAAATCAAGTTCCAACGCACAAAGGAAAGCAAATATCCGTAAATGAATCTCGCCATGGATCCATTGACAGAGTGTCGCAAAGAAAACCACAAGCGGGAGAGAGGAGTCAATGAACTTGGCTTTCGGCTTAGGGAATTCTCTTGGCCAATTTCTTAAAGCCCCTCGGAGTTCC

CCTCTCGCCGACTGCATAAATCGATGACTATCAAAGCGTTTTTACAGCTAATGCGGCTATTTTTATTCATTACGTTTTCTCGGCTTTCTTCCATCGATTGGCTCCGTTTGGCTGGGTTCCTCGGCCAGTCGTTGACCGCAGACGGTGTGATTCTGCTCTTCCGCTGGCCCTATCATTACTAGTCAGTCTTTCTAGCCTGGTTTTCTTCAGTATGGCTTGAAACTCGTCTCTGGCTGCCAAATTGTTTGCCCAGCATTATTGTTTCCTCTCACTCTCGTGCAGATTTCACAGCTAGCTGTTAGTTTTAATAAACAGATATATGTATACTCGCTATGCCCGAGTCATTTTGCAGTAACTTCTTTTTATGGCTCATTTGCATGAGACCAGTTCGAATACCGTTAGGTCATCAAAGGTTCCAGCATCGTTATAATTTGTTTCCTCTGCTTCGCGTTTGGATTCTACAATATATTATATCGCTTCAAAATAACATTTGTTAATCATTGGAGTTCTTTCACTTCTGTGATCTCTCTTGGCTTATTTGCTCAGAAATTTGTTTCATAATGAGGTGGCAGCTCGTAAATTTAATCATTTTTCTTTTCTTTTCACCGACGGTGCGTAAGGCCTTTTGTTTGGGCCTCTCTCGAGAACCCATAAATTAATTATGACCCCTGCGCTAACTGCCGCTGGCCTCTGCCTCTGATCTAATCCCATCGATATTCAGGTTTTAGCCCGTCAACTTAACACAAATTAGCTTAATAACAAATGATGTTTATGGGTTACTTACTGCACTCGAGAGTAGTAGCACTATCCACGTGACTAGTGGCAAAAATATTTTCATTTTTGCTGCAAAGAGATCAAAGAGAAACAATCGCACATTAATAACCATTGAAAAATCAATTATAGCATGTTGGGTAACAGAAAATAAATACAATTAAGGCCACCCGGCAATAAACACTTGAGCCATAAGACGCTTTTCGGCTCTGGCCGAAAGTAAAACAAATAAAAATAATTAACTTAAAAATGTTGGGCACGCACAAACAAAACACAATTGGCCTGGTCATCTCGAAC

 

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