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CRIMIC CR01050

Gene Snoo

CG34421

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2L:7934193..7934220	28D3	FBti0202665	81161

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GTGAGCACTGCCTGTATTAGTGTTCC	TGGGTGTGGGTTTCGGTTTCGGTTTT

Images

Construct Design Sequences

sgRNA sequence	GGGGGGGGCGTACTAAGGTGAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TCTGTCGTTTTCGCCATCGA	GTCGTAATGAATTGGCCGCC	TAGCCAGGTTACCCCCAAAC	GTAGTGTGGAGGGTCGGTTG

 

Amplicon 5'

Amplicon 3'

TCTGTCGTTTTCGCCATCGACCTAAAAATCAGTTAGCCCACTGTCTGTGTGCGGCTATGTGTGCTTGTGTGCGAGTGTGTATGTACGCCAGTCTGTGTGTTCGCCCCATGGGCATTGGTGTGTGGTGTGTGCTGTGTATAAGTGTGTGCTCTTTGGGTGTGGTGTGTCTGTGTGAACAACTGCAAAAAGGAGTGTCTGAAAGTAACGACGACAGCAGCAGCAGCAGCTGCGCCAAACAAAGCAAAAAATGTGTAGCATACATTTTGGGGCAGGCATTATATAGCGCGGTTGAAAGGAATATTCCGATGGAATTCTTTAGCTTATGTACGCAAACGGGTATATAGAGACCCGCCCGCTCGGTAAATGGCGACGTTAAAGGTAACTTTAAAATTCAGCAGACCAGACGCGGCGAAAAGTTTCTGGAACAGATGCAATAAATAAGCGTTTCGCCTTTTTGGGGCCATTAAGAGGAAAACTGGGGTTGCTGCAAAATAGAAATATTCATACATTTCCGTGATAGAACTTTTAGTATCTTAGCTTTGCTGAATTTTAATTTATTTAAGGTTTACGAACCATTAAGCAACCAAAAACTTGTGATGTCTTATTTAAGTAGAACACTAAAATGTTCTTAAATTTTAAGATAATTTTTATTCTTCTATAAATACTATTTCGGACCAGTATTTTAACCAATAGAAGTGACACAAGAGCGACCCAGTAAAAGCCGCTGAAAAGTGGGCAAAATGGGCGGAGTTACAAGAGGTCAAAGTTGGGGGATGCATGTCAAGAGCTCATGACACTGACTGACCGCATTACCCTTCCACAGCTACCCGCCGTGCAGAGCAGACATGTCAGACATCCAGGCGGCCAATTCATTACGAC

TAGCCAGGTTACCCCCAAACCCCCTTCAGTAGCCCCTGATCGATGGCTCTCTGTGGTCACGCGACCGTCGCTTCATCGCTGGTCGACCTACATTCAATTTGCGAGCGACCGCAAAACGCGTCCGACAGTTGACGATTATGACCGGTCCGATGATGATGAATGTGATGCAGGACTTATACCGAGAGATTCCTCATCCACTGACAGATATCGGCGGAAATATGATTTGCTGCAAGTGGGGGATGGGTGGAAGCGCAGTGGATTCTGTGGGTAAGCGGATTTCAGTTCCGTCCAACTAGAGCTGCTTAGTTGCCAGTTGGATGGCTGTGTCACTGGGTCATGCGATATGATTTCTGGCATTTGATTTAAGTCACTGAATGTTGATTTTCATATGCGCAACTTAGATGCTACAACATTTTAATTATATGATAGCTTTGGCATTGTTGCCGTTAAGTATACGCCAGGTGGTCACCACTAATTTTCCTTATATCAAATTAATTACTTAATGTTGCGATGCCTAAGCTCACTTAATTTGCACAAATGGCAATAGTTTTTATCTTGCATCACAAGAAACCGCAAAGGAACCTTATTTACCAGGATCCTTGGGTATCTTTCTTCCTTCCTTCTGTGGTTCCTTTTGTTGTTTTATGCATAATGCATTTGCCACAGTATTGTGAGGCGAAAATCAAACTGAAACTGAACTCTCGACCCTTTGATGGAATGCAAAGCAAACGAGTGTGCGAGTGCATTTTGCTGGTTGCAATTTTGGTTATGGTGCACAGTCCCTTCTTCATGGCGGTGACCTGATTCCTGGTTTTGGCCCATTCCTGCTCCTCTTGGCCGACATCTCTCCTTTTCTTTTGCCAGAGCAAGAGTTGAAGGTCGTTTTGTTTGAAATGGGAAATGAGAAACCCGCAACAACCAACATCCACCAACCAGCAACTGAAACTAACAGAGTCCTTTTTCTCCAACCGACCCTCCACACTAC

 

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