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CRIMIC CR01052

Gene Jhe

CG8425

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2R:16120832..16120845	52E5	FBti0202717	81162

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GGGTGGTGGTGATAAAAGCGAAAGTG	AGGGGTTGCTTGAAACCCCTATCAGA

Images

Construct Design Sequences

sgRNA sequence	GCCTGTAGACATTCAAGTAGAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TTCCGTGTGAATGCATCCGA	GCAATCCTCATCGCCGTAGA	GTCTACAGGCCAGAGGTGAG	GCTGGCCCTGGAAATACACT

 

Amplicon 5'

Amplicon 3'

TTCCGTGTGAATGCATCCGATAGCAAAATCGTGAGCCGAGACAGGATAAAAGCAAACATTTCGATACGATGCGATCGCAGTTGATCGCAAGTCGTCGACTCTGACGGATCGGGGGCATAAGTATAATCCACTGCCACTTTCTACTCGTTCTGGCGCGTGTTTCAAATTACCCTAACCAGATTCGGTTGAGGTCGCTGGTAAGATGCTAGTTGCATTTCCAGATCAATTCCTATTCGCAGCAATCAAGCATACGCCGTGTGTACCGTTCTACAAACGATCCAAAACGATCCCAAACAGCTGGCTAAATCGCGCTGATCTGTATAAATTTTCCACCGCACATTTTGTCAAGATTGTGGGCAGAGAGCCACTTGGTACGGAGCAAAAGTTTTCTTCACGATTCAACTGAACAAATCGTCAGATCGATTTGGGCCTCGGTCGCGATAGGTTCTTCCAGCGATTTTCACCCAGCGACTGTCTAAGTCTAAGATTGAGTGAGTAATTGAGCTTTTCCACCAGGATAGTGGGCCTGATGGCTGATGGCTTATGGTCAGCAAATCCATTTAAAGACCAAATTAAAGTTGACATCATCTTGAGCGAGTTAACGGGCAGCATAGGAAGCCCCAACAAAAAAAAAATTGATCATAAATAACCAATCGTTTTGCATAATTAACGATCCATTAGCTCTATTTTCAGTGATTTCGTGGACCGCCGGCGATGATTCAACAACGGATGCTACAACTGCTGCTTCTTGGACAGCTCTTGGCTGGCCCTGGACCCTTCTGCGCGGCCTTGGCCACTGTGGACCAGCTCACCGTGTGCCCACCGAGCGTGGGCTGCCTGAAGGGCACCAATCTCCAGGGCTATCAGTCCGAAAGATTCGAGGCCTTCATGGGCATTCCCTATGCCCTGCCTCCCATTGGAGATCTGAGATTCAGTGTATGTTAATTGGGCATCTTTGCTCAGAGATTCCATTTATGTACCAGCTACTTCTTCGTCAGAATCCCAAGGTAATGCCCAAGTTGCTGGGGATGTACGATGCCAGTGCGCCCAAGATGGACTGCATCCAGAAGAACTATCTGCTGCCAACGCCAGTTGTCTACGGCGATGAGGATTGC

GTCTACAGGCCAGAGGTGAGATAAAATCGAATTTCTTAGGAGGATCTAAGAAAGTACAGGGTCAGTAGGGATGATCCGGCTTTGCGAAATCCTGTCCAGCTTAAATGATTGCCTTATTTAGATTCGAAAATCCGCACTACCTGTAATGGTCTATATCCATGGCGGCGGCTTCTTTGGCGGCTCTGCAGGACCAGGGGTAACTGGACCCGAATACTTTATGGACTCCGGTGAAGTGATTCTGGTGACCATGGCCTATAGGCTAGGACCCTTTGGTGAGGCCGAAGATAACTAACAAAGTGTAAGAAGTGTAATTTTTGAGTTTCTCGTTCCACACAAAGGATTCCTATCGACGCAGGATGCTGTGATGTCTGGTAACTTTGGCCTAAAGGATCAGAACTTGGCACTGCGTTGGGTGCAGCGCAACATTCGTTTCTTTGGCGGCGATCCTCAGCGGGTGACCATTTTCGGCCAAAGTGCCGGCGGCGTGGCTGCCCACATGCACCTGCTGAGTCCCAGATCCCATGGCCTGTTCCATCGAGTGATCAGCATGAGCGGCACGGCCAATGTGCCCTTCGCCATTGCGGAGCAGCCACTGGAGCAGGCTCGCCTCCTGGCGGAGTTTGCAGACGTGCCAGATGCCAGGAATCTGAGTACAGTAAAGCTAACCAAGGCACTGCGTCGAATAAATGCCACCAAGCTACTGAATGCCGGCGATGGTCTCAAGTACTGGGATGTGGATCACATGACCAATTTCCGTCCAGTGGTCGAGGAGGGATTGGAAGTCGATGCCTTTCTCAACGCGCATCCCATGGATATGCTGGCCCAAGGAATGCCCACATCCATTCCCCTGCTTCTCGGCACTGTTCCAGGTGAGGGTGCAGTCAGGGTGGTGAACATTCTGGGCAATGAGACGCTACGCCAGAGTTTTAACTTGCGATTCGACGAGCTGCTGCAGGAACTGCTGGAATTCCCGGCCAGCTTTAGCCAGGATAGGCGCGAAAAGATGATGGATCTGCTGGTGGAAGTGTATTTCCAGGGCCAGC

 

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