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CRIMIC CR01053

Gene Debcl

CG33134

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2R:6651795..6651815	42B2	FBti0202666	81163

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
no band	short band	GGTCATGCATCGGAACATGTGCACAT	ACTCAAAATATTGGCACCTCCACGGC

Images

Construct Design Sequences

sgRNA sequence	TACAGGATTCCGTAGACACCTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TTTCGCCGGGCTGATAACTT	TGGGCTATTTCAATCACACCTCT	AAACGGATTCAGAGCGACGT	TAAACGACTGGTGCCCATGG

 

Amplicon 5'

Amplicon 3'

TTTCGCCGGGCTGATAACTTCTTGAGGAATTTATCATTAAGCGCATTGCGGAAGTTTTCTCAGAATGCAGTCCTCTAAAGTAAACGCCATTTTCTCTGACAAATCAGATGGAATGGGCCATAACCAGGCTGCATAGAGGTTTTCAAGGAAAATAATAGCAACTAAAGAAAAGGGAAGAACCGCGAAAGCGCCACGGGTGGAACCATTATAAACAAGTATTCCAAAAATAAACCGACCACTAACTATACAACCCGAAAGCCTCAGGTGATGAATTCATCGAACTCTGGCCATTCAAGTAAATCTTATGGTATTTGGTCGCACCAAACCCGCCAAAAGGAAAGAGTTGGGCGCACAAGTGTTCGCCATAACAAGATCACGTAAACAAATTGACGGATTTATTAATATTTCGCTGGGAGGCTGGACGGGTTCGGAGTAGCTTCGATAGACTTTTCAAGACACTAGCTTCAAGGCCGGTGATTTGGCATGGGCTTGATTTCTTTGGCTGGCCATGTCATAAGTCAAGTTGGCTCCAAAGTGAGTAATTGTCTGTTTACTTGGCTGCGGAATCGTTGGCGTGACGAACGGTAATTGTGCGACATAAATTGTCGAAGGAAACTTGCAACTAAATGGGTCGGAATTGAACGCAACATGGTGGGGAATGCCTTAATTACGCCAAACGTTTCGCGTCTTTCAGATTCCATAAGTAAATTTTTAGTTGTCTTAATGCCTTTCCACAGCACGTGCTGATCGCTTCTTTTGTTTAAGTCGTTGTTCAAAGTTTATTACCGCCATGTGCACCTGCCATCAGTTTGGCTGCATGTTTAGATACTCTGTAAGAGGTGTGATTGAAATAGCCCA

AAACGGATTCAGAGCGACGTAAATAGAACATATGCATGTTCGCCGGAAAACTCGAGGCAGTCGATTGATATTGTTTAAGTGGCCCGCTGATGGAGCGAAATTGCCTGGCAATGATCAAAGCACTGTAGTTAATTTGATTGTGATTGAACTTTTGGCTTTGTCGTTCTATTCCAGGGGAAATGTCTGTGTGGTCAGTACATCAGAGCGCGGCTGCGACGGGCAGGAGTCCTCAACCGGAAGGTGACACAGCGTTTGCGCAACATCCTGGACCCCGGCTCCTCGCACGTGGTCTATGAAGTTTTCCCGGCACTGAACAGCGTGAGTGTACTGCCATTCGCCATTGAATCTTTTCATAACATGGTATAATCCACAGATGGGCGAGGAACTGGAGCGGATGCACCCGCGGGTGTACACAAACATATCGCGACAGCTGTCGAGGGCCCCGTTTGGCGAGCTGGAGGACAGCGACATGGCGCCCATGTTGCTCAACCTAGTTGCCAAGGATCTTTTTCGCTCCAGCATCACCTGGGGCAAGATAATCTCGATATTTGCCGTATGCGGCGGCTTTGCCATAGACTGCGTGCGCCAGGGACATTTCGACTACCTACAGTGCCTGATTGACGGTCTGGCTGAGATCATAGAGGACGACCTGGTCTACTGGCTGATCGACAACGGCGGATGGTTGGGCCTGTCGCGGCACATCCGACCCCGGGTCGGCGAATTTACGTTCTTGGGATGGTTGACGCTGTTCGTGACTATCTCTGCAGGCGCATATATGGTCTCAAACGTGTGTCGGCGCATTGGAGGTCAACTGTATTCGCTGCTGTTCTAGATTCGCTTGGGATCGCGTCGTTAAGAAATACAATCGTACCATTTAGTCAATGAGAGCTTCAAATCATTCCTGCTTCCATGGGCACCAGTCGTTTA

 

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