CR01054

CRIMIC CR01054

Gene CG13744

CG13744

Information

Donor Location Cytology FBti Stock number
pM37-p02R:9015181..901598945A1FBti020271881164

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
short band OK CAGAACCAAGATGAACCTGTTGCTGC TCCGGAGTCACCTGGGATACGTAAAT

Images

Construct Design Sequences

sgRNA sequence CGTCCGAGGAACTCCACACTCGG
sgRNA2 sequence
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
TGCGAAACGGGGCTTAATCT CGCAGAACACGCTTCAGAA ACTCGGGCTGGGAAAATGTTA TGGCTGCTGGGAAAAGGAAA
 
Amplicon 5' Amplicon 3'
TGCGAAACGGGGCTTAATCTTGTTGTTACGTTAGTATGTTTTAAAAGTTTAGCTTAATGCAAGATTATATTACTTATCAGAAGTATGTAAGCCAAAAACAAACACTAAATTTTAAAAGCTAAGCCTGAAAGTATGCTGTGATACCTGGCTGAAATAAGCAACGTCTCATGAGATGAACCGATTGTGGTCTCTGAACCAATTTTAATTTAGCGGAGTAGTAAATATATTTAAGGGTCGGGAATCTAACAACTCATTACCAGATAAATATTTCCCTTTAAAAACAGGTTTACTTATAAACGAACACATACAATATACAATTTATATTTTTTTTGCTTAAGGACGTTGGAATGAAATAAACCAACATTTTAGTGGCATTGACTCACCCAAGCAGGCATCCATGTGTCCATCGGAGTGTCCGGCGCAAAACATTTCGGCCTTGATCTCGACATTTATCTGTTTGCTCTCGTGCCAGCGAATGCAATCCAGTGTGGCTGAAATACAAGGTATATATTATAACATAATTTAATAATAGTAGGATTCAGCACTCACTTATGATGGGAACACTGGCGACTTGCAGCATGTTGGTGCCCGCGTGGCCCATGTGCGCCTCCGTCTTGCCCCATCCTGCAATGAGGCCCTTGCGCCCAATCAGCCGGATCGGATACTGCGGCAGGCAGATGGGCAGGATGTGCTCCGTAAAGGACGTCGGCTGGGCCAGCTTCAGCAGGGCTATGTCATACCTATCCGGCTGGGTCATGCGGAAGTTGAAGCGCGGATGGATGATCTTCTGTAGCACTCCGTGCTTCTCCACGGGCAGCGGTTCGTGTATGTGACCCAGATCCTGCGTGTCCAGCTCTCCCAGGTACACGGTTATGTCCGCCAGGTGGGCCTGCTGGATGCAGTGGGCGGCAGTGGCCACCATGTTGGCCGAGATGAGCACTCCACCGCATTGATACTCCGCGATGCGGATGTGCGCCTGCCACGGATACTCCGCAAACTGCGCTGGCCGACCGCCAATAATGCGCTTCTGAAGCGTGTTCTGCG ACTCGGGCTGGGAAAATGTTAAGTATATTAAGTATGGTACATGTAACATGAGTCAATGCCATTTAATGTCTATATGTACATTTAGGCACTCAACTTTACATATGAAAGTCGTACACCGAAATGGAAAACCACTGAAAATAAGTAGCATCTCCATTCTAGCTCTAATTAGGAAAATCCTGCCCGTCTGTCAAATGAAATTGCTCAATTATCCCGCTCTGCGGTCGCAATGGCTCAATTGCCGCAGCACAGCAGACCCACCTTGGGATTGAGCAGCTCGTTGTCGTCACGTCGGCGCAGCATGATCCGCTTGGGCAGGCTGTTGAGGTTCAACTTCAGCTTTCCATAGTCCACCAGGTTGGCCAGCGGCGAGGAGGGGGAGTGATGCTGCTGCTGGTGCGGATGCTGTGTCTCGGCGACGCAGCAACTGAAGAGCCACTTGTTGCTGCCACATCCCTTTGCGTGCTTGCCGCCTTGGAGCCAGCAGGAGAAGCTCAGCTTGCAGGGCCATACGCCGCTCTGGTGGACGCATTCCGCGGGCACCCCCAGTAGGGTGTTCAGAATATCTGGGCGGGATTGGGTGACTAGACGTGGAGTTCCTAGCGCGCGTGCATATGTACTCACTGGCTTGACTGGGACTAGACTGACAGATGAGCAGGAGGAGGCACAGGCACAGCCACAATAGCAACTGCATCTTGGCGTCGGATTATTCGAGGTCACTATTCGCACTTCGCTTCTCCCCGAGGAGGATAACACTCAAACTGAAACACTCATCAGAATGGACTATAACGCTTTTGTTGCCAATGAAAGCATGACAGTTTCTTATTCACTTTTAAGCTGGCTCTGCGCTGCCGTTTTCTTCTTACGTTTCCTATTATTGCTGCTCCACTTTTACTAGAAGTCGGTGCTGTTTATGGGCCTGTCTGGGTGACTCACGGAGCTCTCGGGGAGCTGCTCGGCGCAAAAGAGATCTGTGTCCTTGATAGGCGAACAAGTTTCGGGGTCGCTTGCAAATGGGTCGCTGTTCGGGGCTAATGGATTTTTATATAACAATCATAATGGGAACCATGCCAATTCTGTGCCGACTGACTCGATGACACAGTTTTCCGTTCAGTTTTCCTTTTCCCAGCAGCCA
 

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