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CRIMIC CR01054

Gene CG13744

CG13744

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2R:9015181..9015989	45A1	FBti0202718	81164

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
short band	OK	CAGAACCAAGATGAACCTGTTGCTGC	TCCGGAGTCACCTGGGATACGTAAAT

Images

Construct Design Sequences

sgRNA sequence	CGTCCGAGGAACTCCACACTCGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TGCGAAACGGGGCTTAATCT	CGCAGAACACGCTTCAGAA	ACTCGGGCTGGGAAAATGTTA	TGGCTGCTGGGAAAAGGAAA

 

Amplicon 5'

Amplicon 3'

TGCGAAACGGGGCTTAATCTTGTTGTTACGTTAGTATGTTTTAAAAGTTTAGCTTAATGCAAGATTATATTACTTATCAGAAGTATGTAAGCCAAAAACAAACACTAAATTTTAAAAGCTAAGCCTGAAAGTATGCTGTGATACCTGGCTGAAATAAGCAACGTCTCATGAGATGAACCGATTGTGGTCTCTGAACCAATTTTAATTTAGCGGAGTAGTAAATATATTTAAGGGTCGGGAATCTAACAACTCATTACCAGATAAATATTTCCCTTTAAAAACAGGTTTACTTATAAACGAACACATACAATATACAATTTATATTTTTTTTGCTTAAGGACGTTGGAATGAAATAAACCAACATTTTAGTGGCATTGACTCACCCAAGCAGGCATCCATGTGTCCATCGGAGTGTCCGGCGCAAAACATTTCGGCCTTGATCTCGACATTTATCTGTTTGCTCTCGTGCCAGCGAATGCAATCCAGTGTGGCTGAAATACAAGGTATATATTATAACATAATTTAATAATAGTAGGATTCAGCACTCACTTATGATGGGAACACTGGCGACTTGCAGCATGTTGGTGCCCGCGTGGCCCATGTGCGCCTCCGTCTTGCCCCATCCTGCAATGAGGCCCTTGCGCCCAATCAGCCGGATCGGATACTGCGGCAGGCAGATGGGCAGGATGTGCTCCGTAAAGGACGTCGGCTGGGCCAGCTTCAGCAGGGCTATGTCATACCTATCCGGCTGGGTCATGCGGAAGTTGAAGCGCGGATGGATGATCTTCTGTAGCACTCCGTGCTTCTCCACGGGCAGCGGTTCGTGTATGTGACCCAGATCCTGCGTGTCCAGCTCTCCCAGGTACACGGTTATGTCCGCCAGGTGGGCCTGCTGGATGCAGTGGGCGGCAGTGGCCACCATGTTGGCCGAGATGAGCACTCCACCGCATTGATACTCCGCGATGCGGATGTGCGCCTGCCACGGATACTCCGCAAACTGCGCTGGCCGACCGCCAATAATGCGCTTCTGAAGCGTGTTCTGCG

ACTCGGGCTGGGAAAATGTTAAGTATATTAAGTATGGTACATGTAACATGAGTCAATGCCATTTAATGTCTATATGTACATTTAGGCACTCAACTTTACATATGAAAGTCGTACACCGAAATGGAAAACCACTGAAAATAAGTAGCATCTCCATTCTAGCTCTAATTAGGAAAATCCTGCCCGTCTGTCAAATGAAATTGCTCAATTATCCCGCTCTGCGGTCGCAATGGCTCAATTGCCGCAGCACAGCAGACCCACCTTGGGATTGAGCAGCTCGTTGTCGTCACGTCGGCGCAGCATGATCCGCTTGGGCAGGCTGTTGAGGTTCAACTTCAGCTTTCCATAGTCCACCAGGTTGGCCAGCGGCGAGGAGGGGGAGTGATGCTGCTGCTGGTGCGGATGCTGTGTCTCGGCGACGCAGCAACTGAAGAGCCACTTGTTGCTGCCACATCCCTTTGCGTGCTTGCCGCCTTGGAGCCAGCAGGAGAAGCTCAGCTTGCAGGGCCATACGCCGCTCTGGTGGACGCATTCCGCGGGCACCCCCAGTAGGGTGTTCAGAATATCTGGGCGGGATTGGGTGACTAGACGTGGAGTTCCTAGCGCGCGTGCATATGTACTCACTGGCTTGACTGGGACTAGACTGACAGATGAGCAGGAGGAGGCACAGGCACAGCCACAATAGCAACTGCATCTTGGCGTCGGATTATTCGAGGTCACTATTCGCACTTCGCTTCTCCCCGAGGAGGATAACACTCAAACTGAAACACTCATCAGAATGGACTATAACGCTTTTGTTGCCAATGAAAGCATGACAGTTTCTTATTCACTTTTAAGCTGGCTCTGCGCTGCCGTTTTCTTCTTACGTTTCCTATTATTGCTGCTCCACTTTTACTAGAAGTCGGTGCTGTTTATGGGCCTGTCTGGGTGACTCACGGAGCTCTCGGGGAGCTGCTCGGCGCAAAAGAGATCTGTGTCCTTGATAGGCGAACAAGTTTCGGGGTCGCTTGCAAATGGGTCGCTGTTCGGGGCTAATGGATTTTTATATAACAATCATAATGGGAACCATGCCAATTCTGTGCCGACTGACTCGATGACACAGTTTTCCGTTCAGTTTTCCTTTTCCCAGCAGCCA

 

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