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CRIMIC CR01055

Gene CG15615

CG15615

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2R:16793828..16794696	53D9	FBti0202719	81165

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
multiple bands	OK	AAGCCTTGGGCTTGCAAGGTGATTTG	GGCATTGGTGTCATCTCGTTTCCAGA

Images

Construct Design Sequences

sgRNA sequence	TCTGCTCGGTGTGCTCTGTTTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GCGAGGGGAACCATTCTGAA	CATCGGAGGCAGTCAATCCA	CCCAACTGAAATGAGCGTGG	TCCCTTCCGACAAATGAGCC

 

Amplicon 5'

Amplicon 3'

GCGAGGGGAACCATTCTGAAATGACCACTGGCTTCCAGATCTTTTTCCATCCAGGCACCCAGATCTCCTTCCAGCCGGGCACCCAAATCTGCTTCCATGCCTCCTTCCATTCCTGAAAAGAGTTGGAATATTTAGTTCGGATTTTATTTCTGATTAGTATGATAAACATACCAATTTATCGGTCCAGATTTCCTTCTTCGCTGGCTTCCAGTACTGCTTCCATGCCTCCTTCCACTCCAATTTCTTCTCTGGCACCCAGATTTGCTTCTTGGCTGGCTTCCAGATCTTCTTCCAGTGCGATTTCCAGACCACTTTCTAAACATTCGGGATTTAAGTGTTTAAATAACATCCTTAAACTTAAAATATATTTTACAACCAACCTTCTTCCACAAATCGTGGCTGGTGTACTCCCAACCTTCGTGATCCTTGCCCAAATATTTTTCTCCGGGAATGCCAACCTTTAAATTAACATGATTCTTAGCTACATGCACTTAAAGGTAAATGTATATTCCATTATACCTTGACTAGTTCTGGTGTCCAGATCTGCTTGTAATCGGGTACTTGAATATCCTTCCAGGCTGGCACTAAAATCTCTTTCCAGTGAGGAACCTAATATACAGAGTGTATATAGTTAATTGCACTTTCATTGAGAGTTAAATTTAGAACATACCCAAATTTGTTTCCAAGCTGGAACCTGTATTTCCTTCCAGCCGGGCACTTTCACCCAATGTGGCTTCCAGATCTGTGATAGACATAATTAAAATTGTACTCAATCCCTAAATATATTGATTATTTATTTTATTATAGACTAAGCTATTTACCTTCTTCCATGAAGGATTCCAGATTTGTTTCTTAGCCGGATTCCAAATTTTCTTCCATCCCTCCTTCCAAGTGACCTTCTTCTTCCAGTACCCGGGGTCATGGTGCTCGTGATGATGTTCGTGATGTTCGATTATGTGCTCGTGATGGTGGTCGTGGATCAGGTCATCGCCCAGACCCAATCCCAGATCGATCCTCTGCTGAGCGACACTTTTGCTATCCACCTGGGAAGCTGCAGGCTGCTGATCCACCACAGCCTTGGATTGACTGCCTCCGATG

CCCAACTGAAATGAGCGTGGAAGTTTATGCTTGTAGTTAATAGATACAAATCAAAAGACTGAGACAACGAACTCGGGCATGAAGAAAGCTTGTTTAAATTGAATTCGCAACGATCATGTTAATGAATTCCATATTCTCCAAAGAAAGGTGAGTGACTGAAAACCAGTTTTAAGTGGGTCAGAGTCTGGCATTGTTTTTTGGGCGACCGACAAAGAAATGTTGCATAACCCCAGATGTTTATAATTTCATACAATGCCTGACCTGGGTCAAATGAGCTAGCTGATGACAAATCATGCTGGGCCAAAGACTTTTCGGGTCTGGGCCCTCTTTATTTGGCCAATTTGTTTTATTATGTAAATGGGTTTTTGTATTATTCGGATAAATATGGAACTGTTGGATAAATGACGCAAGCCATCTCTGCATTTGGAGAATCCAACAAGGTCAGGAAACGCCCTCGTGATTTCGCAAAATTAGCCCGATTTGTGGTTACAATTTCAAAAGACCCACAACCAAAACACAATTCCAATCAATTTGCGCATAAATGTCAACGAGACCCGGCCCGATTATGAAATGGAAATTAGCGCCAGCAAAAATTATCTCATCGGATAGTGCTAATTGTAGCAGATCGATCGTGTCAGCTTCCTCACTTTGGGTGGGCAGTGAAAGCTACGCAGACTGGTTTCGCCTGCAGTTGGATCATCGGTCGTGAGGGAAAAGAGTTGGCGACTCCGCACTGCACCCCACGGATATATACTATATGTATGTATATATCTATATAGTATGCCCCCTGGTCGCTAATGAAACGATCGCGATTTTAATTGTTGACAATGTGGCTCATTTGTCGGAAGGGA

 

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