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CRIMIC CR01056

Gene Lpin

CG8709

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2R:8140372..8140381	44B4	FBti0202667	81166

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CGGCGGTCATTGAAGACACGGAAAAA	AGGAACCGCCAGACTTACATTGATGC

Images

Construct Design Sequences

sgRNA sequence	CCTCATTTGTACCTGTGCGCAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GGTGGAAGGCCGATATCAGG	GGTGTGCAAAAGCCCCAG	GGTACAAATGAGGGCGTTGC	ACTGGGAGTGAGCAACATCG

 

Amplicon 5'

Amplicon 3'

GGTGGAAGGCCGATATCAGGGACGTGGGATTCAGCAACAGCGGTCCGTCCGGCAGCATCACGTTGCCCTGCCGGATCGACCGGAGGTACTCGCGTGTCACCCTGCTTTGGCCGATGGCACGGGCTGACAGATAGAGCAGCTTGTAGCCGTTTTGCTCGATCTTCGAGAAGAGCTGCGCCACACCGAGTTGCGCCCAATCCTTGCCCACCATGGGTAAAATGTGGCCCAGCACGTCCGACTTGGTGATGGTGCCGTCAATGTCCGAGATCACCACCTTGTCGTTGTGCTTCCAGCGGAACAAGTAGCACTTGCAGCGCGTCGTCCCTTGATAAGCGGTCGTTACGCTGAACTCGATTTCATTCATGCCCTCCTTGAGGTTCAGTTTTTTCTAAAGGAGAATAGGTTTTGTTAGATTCGCATTGAAGCTTGCATTGGTGCACTTACGATAGCCGCCGAGCTAAGTCGCAGCGACTTCTTGTAACGCTCTTTGGGCTCGTCGCTCTTGTTGGAGGCCATGGTTAGCTCCTCCAGGTTGTCCACCAACGCCGAGGTGTTCTCCGCGTTCACCAGGGAGTCGCTCTTGCTCAGCGTGGGATCGGTGATGTCGGGCGAGGTAGGCCGCGAAGTTTGCGTTGCCACAGCTGCCTGATCACCATCTGAAAAGCAGTACAATCTTTAGATGGGCTGTTTATTAACATTTGGGGCTACCCTTGATCTACCTTTCTCATCCTTGCCCAAAGGCATACCATGAGTGTTGTTCAAGTGGTTGGGCGCAGCGTCCTGCGAGCGTCGCCAGCTCCACCAGTAGCGCTTCGTCTGACCCCCATTGTCGGCCTGGGCAACTGCCTCCTGCTTCTCGTCGCCAGGCAGACACTTGCCGTCGACTGTCTGAGACATCAGCTGCTCAATGGCATCCTGACAAATGGCAATTGAGGACACAATCTAATTAGAGCCGAAAGCGAACAAGGCATGGGGCTACACCAAAGGCAAGGCGTTGGGCCAACTTACATGGGTTAGTGGCTTCTGGAAGGTGATCATTGTCATGACAATGGGACATGCAGCCATCCAGGTGTAGTATTTGCCATTCAGCCGTACGACTAGGTTAGGCGATGAAAAAATGCTGGGGCTTTTGCACACC

GGTACAAATGAGGGCGTTGCATGGGAAGAGACTTGTTAACAAGGCGGGATGCAGTGCGTGGGGGCGCAGTGAAGTGCTTGGAACTTAAACACATACACAGATCATAGCACTGGGCCATTTGAACAATACTCACGTCTGGATAGTTGACCAGGTGGCGGTCGAACTCCTCGTCCGAGGGTGGTGCTCCCTGCTCCGACATTCCGCACATGGACATGGCCACAAAGTCCAAGTACCTGCTGGCAGATTATTGATGTGAATCTAGTATACAAATTGGTGTCTGACCCCGATAGTGCTACTTACTTGTTGTCCCTCTGCTGCTTGGTCTCGTCAAAGTCCGAGTCAATACTCTTCTGACCTTCCTCCAGCGAGCTGGGCGAGTGAGGCAGACTGGTGCCATTGCCGCTTTCGCCATCCTCCTCCGGCGGTGATGCCGCCTTGGACAGGGGACTAGGGAAGTAGAGGGCCGCCATCTCGGGGTCCATGCTGCCGGCATCCAGATCAGACAGGTAGATGTCACCCACTTCGCCGACGCCCTTCTCTTTGCGTAGCCGATTTGCCCTCTTCATGAAGCTGAACATGTTGCTGAGCATCGATTGGTGCTCGCTTTGCTGCACCTGGGCGGCGCTCATGGCCTCATTCTTGGCCTGCTCCGGTGTGGGCAACTCGCCCCACTTCCACGATGCGGTGCTTTCTTCAGTCACCACGTGACGGTTGTCTCGCATGGTGGTTTCCAGTTCACTGTCACTTTGGATGGGAGTCGAAGGTCGTCCGCCGGCGGCACGACCTGACCCAGCACCACCGCCACCCACGGGAGTGGTGATCTCCGTGTCGCTGAAGAAGTGAATGTCCAAATCCAGACGTGGATTGCTAGTGGGGGTGTGGGGAATCTCGCTGAGCGGACTATCGTCCCCAGTGCGAGCTGTCACCAGGGGAGCGGATAGCTGTTCATCGTTCGACGACGAGGTGTTGTTGTTGTTTGTGGCACTGGATATGGGGGCATCTCCTTCATCGATGTTGCTCACTCCCAGT

 

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