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CRIMIC CR01057

Gene tutl

CG15427

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2L:4296693..4296715	24E1		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AGCGAAAACGGCAGCATTCACAGTGA	GTCGGAAACAGCAGGACAACAACAGT

Images

Construct Design Sequences

sgRNA sequence	AAGTGGACGAGGCTTGGGTATGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GTCTCATTGCTGGCCCTTCT	CAAGCCTCGTCCACTTCACT	ATGGGAAAACGTCTGCCTTT	GGAACGTTGTCCGGCAAAAA

 

Amplicon 5'

Amplicon 3'

GTCTCATTGCTGGCCCTTCTAGCGAAAAATGCCCAGGCGCACAATATTCCAGGTGAGTGCAATGCAATCGAGTTGCTTAATAAAATCGCTCGAAAGCCTCGACTATGCGTGGCCAAAAATTTCATATGAAAATTTGATGGACGACGACGCTCCTATTGCTCTTTTCATCAGCTGTGGGGCGAAAAATGCGCATGTGTGGAACTTGATTTTTATCTGGTGTTCACTGCTCGGCGCTCGGGAAAATTTAATGGCGCACAATAAGCGGCTTACGTGCGCCCAAAGCTGATTACATTTGGCAATTAGTTGACAGCAGCACCGGCCATCGAGCACCGAGTTCCGACCGGCAAGCCAGTGGCCATTGGCCAAAAAAAGAGAGAGTGGAGCTTTTGTTTAGGCCTCTTGATTTATTCGTTCCCCATTCGTGGCCAGCACAATAGGTGGCCCTAAAATACAATAATTCGTGATTGTGAGCTTCAATAACTGGCTTAAACCATTTACGATAGCCGTTATAGAGATTCCAGTATAAAATCAAAGAGAATTTAGAATCTGCGCTTAAATGTAACTTAAATGATACTTTTACTACGTTTTTGGCCAAATTTAGTTACGTGACTCCGATCTTAAGGATGATGGTTATCTAGAACTTTGAAAATTTCTTTGGAATGCCTTAGGTTAACTGCCCGTCACAGAACCTTTTGTGAACTTCCGCTTTCGTCTAAACAGTCGAATATTCTGCGCTATTTCCTGATAGTCCCTTCTGTCCTGCCGAAGCAGGACATTAAAAGTTGCCACTGGCTCCGCTCAATGTACCGTGACGTTCTTCGTTGCGAGCTCGTCGAGTGAATTAATTAAATTTAATTTAGCCGAAAATTGGAAAAACCGCTTGAGATTCCTTTGCCATGGCAGCGCAATGGCCACTTTGCAAAACGGTCGAAAGGTCAACGGCAGGTCCATGGATTTTCAGGGAGGGGCGTGACCCTGCCCCGATGGGGAAAGAGCCAAATAAGTTTCTCGAAGGCCTCTAAACTTGGCTCAAATCAATGAAAACAAAATGCGCCTCGCTGAATAGCTCAAGCCAAAACTCGTGTCAAGTGCTCGGTGGCGTTGGGTGGGTGGGTGGTTAGTGAAGTGGACGAGGCTTG

ATGGGAAAACGTCTGCCTTTGGCTGAAGGACATTCTAAATGGAATAATGAAACCACTTTTGCATGTTAACGCCATTAAAAGGCGCGACAGAGGCGAAACACACGCGGTTACTTTGACATTCTAGCTTCCTGACTTCCTGCTACCGTCGAGTACTTCACTTGGCTCTCGGCGACATGCCTGCAACATTATCAAAGGGCAATAGACGAACAAGTGCCTTGGCAGTGTGCACAGAAAGAAAAAACTTGCAATTACCATTAGCTCGTTGGTCATTCAAAAGGGTATCTTAAAGGCAATTATCGAACTTCATTAAGCAAAAGTAATGCTGCATAGTTTCAAGTACTTTCAATGGTGACTTCTTCTTATGGGATCCTCCATAACTTATCACTCTTTTGTCTCCGTGCAGGCATCTCAACGTAATCACCCATTGCCAACCCCATCAAGTCGTTGTCGTTATTGTTTTTGTGTCGCTCCTGATGTTGTGCTCTGTATTTTTTCGGAAGCCGGAAAACGGAGGCTGGAGGCTGAGTGTCGATGCAAATAGTCTGCCAAAGCTGGCCAAGTTAACATAAATTTATGCAAATGCCAAAGTCAACAAGCAAAGAGTGCATAAAGCGACCCAGCGAGTGAGTGAGTGATTCAGTGTATGAGTGAGTGACTGACTGGTTGAGTGTCTGTTGGAGAGCGGCTAACAAAGACGTCATGTAGCTGGAGACCAAGTCCTTGACAGGCTTTTTCCCCATCTGCGAGGACGAGAGCTGGTAGGGGAATTAATGCAAAACGCATTAAGTTGATTTTTTGCCGGACAACGTTCC

 

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