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CRIMIC CR01058

Gene Pka-R2

CG15862

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2R:10001079..10001086	46D1	FBti0202668	81167

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AGTTTTTGAGCCCGTTTGCCTGTTGG	ATCGTAGGCCTCGGCGAAAACTGATT

Images

Construct Design Sequences

sgRNA sequence	CAGCGCAAGCACCGAGCGCTTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CCCATGGAAGGGCAACATCT	GTGCTTGCGCTGGATTTGTA	CTTGGCTTTCCCGTCACACG	ATCTCTCGTTGCCGTCACTC

 

Amplicon 5'

Amplicon 3'

CCCATGGAAGGGCAACATCTTCATTTAATGAACGACAAAATGCCCAGCTATCTGGGCACTGGCATCAGCCTGGAATCTGCTGGTCTGGCACATCTCATGCTGCGCCACAATTGCAATTCAAATGAAACTGCAGAGGAGCTCGCATATCAACGACAGGCAGCATAATCCAATGGAATTTAGAGCCATGTACGGAAAACCCAGAAAGCATCTGCACAAATTTTTCACTCAGCTTCAGGCTTTTACACCGGAAAAGATTCATTTCCAGATATTAAGATATTATCAGATATTAAGATATTTTTAAAGAAAGTTATTACCCGGCTATTAAGTGAAATTGAATTCGATACAGCTTTGATGTACCTTTATAGAATTATAACATATTTATTTGTTTGTTTTTCCAAACAAAAAACAATTTGAATCTGTTTTCCATTGTGTTCCACACTTTAAAACATCAGAGTCACTGCTAAAATTAGAATGGGTTGGCGAACTCTTTGACCTTTTTTTGCGTGTGACAATCCCAGATATGTACTATAGTTCGCAGAATGCCTTTCAGAAACAGCACAAATATGAGGCATTGTCCAGCTGACAGAATATTTGTTAAAACATGCGAGGCAATCAAGGCTGATCGTTTCACTTCCCTGCCTGCTACAGATATTTAAATGTTTGCCCAGGTTCACATGACCGCAAAACCGATTGCTGTAATTATACATTCAAACCGAGCACAACAACACCTTAAGTGCTCATGTTTTTGTCTACACACTGTATGTATTTTTGGTCAACGAAATTCGGTTTTTGCTTTGGCCAAGCAAAGCAAAGCAAAGCCACTACTCACCAGCCAATAAATTACCAGACACCTGAGTGGACATTTTCAATTGCACTAGTGCTGCTTTCTGTGCGTGTTTCCGAATTTAGTTACGATTTCAAATCGTTTGCAACGAGCGCGAAAATAAAAACCAGTTAGGCATGAACACGTTTAAAAATAAGTAGAAATAAAAATGTGTTGGGTGTGTATGTAACCCAAGGTGAATGATGTATGTAGACGCAAAAAAAAAGAGTGAAAAAAACCATACAAATCCAGCGCAAGCAC

CTTGGCTTTCCCGTCACACGCTGCGTATGCGTAATAAATATTTATGCACTCGAATGTTTGGCAAAGCCTTCAGCAAGTCAGTCGAAAATGTTTGTATATATGTGTGTTCTGTGTGTTCCACCACATCAAAATCACATAGTTCTGTTGCTGATTCGGTTTCTGATTCGGATTCAGATTTGGTTTTGTATTTGGATTGGCACAAAATGTTTACACTGCAGCCCGCAGTTTTGCACTCACAGTCGTGTCAAGTTGCCAGTTAGAGTGTCAACCTAATGATGCAATTACGCAACAGTTTCCCAGGCACGCATTCTGGATTTCCCTCAAGTTTACCTAAACGCCAAAGGCCACAAAAATTGTAAACCTAGACGGGACAAGTGCACGTCCATTTCCCCTCCAGATAAGTATTTATCCAAGCCCTGTATGTATATACACCCATCTCGTCCGTCTAGTCTGTCTGTTTGCATTGTGTGTGGAGCAGTAGAGACGCCCACGTTCGAAATCTTGGATGGCAATTGCCTCCGTGATCTGTTCTGGTTTGTATTGCAGCCGAATGTGCAAGGTATGTTGAGATTTCTGATTTACAGTCTGGTAATTAGAAGAAGATTTGCAAACGTCTATCAATCACATAAACTTACGTTTCCTTATTTATGGGATATTTGATTTTTCAACATGGAACTATCTAGTCAAAACTGCGCACAATATTTTTCAAAATATTTATTCGATGTTGTTTTGAGACCATCACAAGATTGAACTTTATCATCTTTAGATTTAGATCTTTCTTTGCAACTGAAAAGTAATTAACACTCTAAAATAAAGTGCAACTTCTTCTTTGGTATTCAAATTGAAATCAGATTGTATACTAAGCTAAATCCTTGCTTTGGTCAAAAACGAAAACTAATTGAGGCAAAGTGTTTTGCTTACGGGAAATATTTAGATTTGAAAGATTTTAGATGTCAACTCAGTACGCTGGCAAAACTTTGAATATCATTTGGTAATTGATGCACCGAAAGTATTTCAATTAAGTAAGTTTCTTGCTTCACAAACACACATGCACTCAGGCAAATAAGCTGGGGAATTCAATTAAGGATGGCAAATAAAAAGTTGTCTACATTAATTTCATTAGCGGAGTGAAACAATAGCAACGAGAACGACTGCGAGTGACGGCAACGAGAGAT

 

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