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CRIMIC CR01060

Gene Unc-89

CG33519

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2R:24007514..24008179	60B2	FBti0202720	81168

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	ACATAACCGACCACGCTTCACTAACC	GCTTCCATTGGAAGACTGGCTTCTCT

Images

Construct Design Sequences

sgRNA sequence	GCTTTACGGATGGCCAGAGTCGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CTCACAATTTCACGCGAGGC	AAAACTCGGGACTACGACCG	AGTAGTGGGCCAAAACGGAA	GGACACATCGCTTCGCAATG

 

Amplicon 5'

Amplicon 3'

CTCACAATTTCACGCGAGGCTTGGGATCCCCTATAATCTTGACCATGAAACGGAAGAGAGTGTTCTCGATCATCTCAGCATCTTTCAAGCTAACCAGGAAGGCTGGCTCCGTATTGGTTTTGGTTTCATCATGACCAGGAGCTGCACGGGAAATGGGCTAGTCATCTCATGGCTGCAAACGCTACCTAAATCTACTCTTACCTTGACCAACCGCCAGTTGTGCCGACACAGTTGTCGTTTCGGATGCAGACTGGGCCCGAATGGTGTAGGTACCCGCATCGGTCTCACTGGAACCAGTTCGAGAAATCATTTAAATCACTTAAATTGTAGAAAACAACAAAGGCACCCACCTGCAGTTCTTGATGTCCAACCGGTAGGAGTTCATGGGAGCGGCCGTTTGGGTGATTCTATCAGCCAAACGATCCTCCAGGGGCTTGTTGTCCTTCAGCCAAGTAACCAAGCCAGGCTGATCCTTGAATTGGATCTCAAAGTGGGCAGTCTCTCCAGCTAATCGGGCAGTTAACATAATCGCTTAAAAACCATATCTTTGAACATGCATGTGTGATAAACTCACGTTCCACTTGCACGCTCTTCAGCATCTTCACCACCACTGGCTTGTCGACCGAGCGCAGGGGTCGATCCTCTTGGAGACGGGACGATGACCGGATTCCGGCACGCGATTCTGCCACAGAGTAGGAGGCACGGCTCTCGGCTCGGCTTTCGGCGCGGCTTGCAGCACGGCTCTCTGTGCGTCCGGTGGCCAGCAGAGACGAACGGGCTTCGACCTTGTCAACGGAACTCCGATCCCGCCTGGAGCTTATGCCATACTGCGATCGCTCAACGGTGCTGTCATAGGAGGTTCCCCGGTCGTAGTCCCGAGTTTT

AGTAGTGGGCCAAAACGGAATATTCAATTTTAGACAGAGGCATTCCTGAAATCCCGAGAATCCAGAGATTCCAAGAACCCCTCGTTGACAACCAATTCTCATTCTAAAGAGGAACTTGCAAGGATGGGATTGGTTTTGGGATTCAATGTGGGAAAACAGGTAGACACACATAAAGTGTTATTTGTAAAGAAAACAACGTGTGTAAACAATGAAGAGTAACACATGAAGAATAGTTTAAACAAAGTTATTTACGATTAACTCAACGGATTTATGTGTAGATGACGTGAATCAGACGTGAATCACATGAAGTTAACCGATGTTTAGGCTGACTTGTCTTGCAAAAAGTTGATTGATAGTTACGACAGATCCCAGATCCCAGATCCTAGACATTGAGTTTAACTGGCTTTAGAACGCACATTCAACACGATCATCAGAGGATGGCTTCCACTAACCCATCAATACATATATGCATGTTAACTACTCCAGACCGATGGGTCCGATCGGTCGGTCGTGACAACTTACTTTCATAGCGACGTGTTGAAGCCCTCGAGTAGCCAGAATAATAATCATCCATACCACCGACGCTTTGGCCCCTGGCTTCGAAATCGGCACTTGGCTCTCTGAAATCGTCAGCAATAGACTCGGTATAGTCGCCTTCAAGCTCAAAGATATTAACGGGCTCCTCAACGATCTCGTAAATGGGTCGTTTCTTAACGGGCGGGGTGAAAATGTGCAGAAAGTCGCGGGCCTTGGCCTTCTGCACAGTGTCTTGGGCATAATTGGTGGAGTCCTTCGACTTGAGCACCGTGCTCTTGATGCTGCGGATCTTCTGCTTGGCATTGCGAAGCGATGTGTCC

 

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