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CRIMIC CR01061

Gene kcc

CG5594

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2R:23911681..23911703	60A10		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	ATGGAAATGTACACCTGCAACGAGGC	CAATGAGACCTTGATTTAGTGGCGGG

Images

Construct Design Sequences

sgRNA sequence	AGGTGATATCCACAGGGCGGAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TGTGTCCAAATTCCCTCCCG	CACCTGAGTCTGTGGATGCA	GAAGTCCAGCATCGACCCAA	AGAACAACATTCCCACCGCT

 

Amplicon 5'

Amplicon 3'

TGTGTCCAAATTCCCTCCCGGAGACCCCAGATCCGGGCACTCGGTGTCGAGGAGGATTACCCCGCATTCCCATAGAAAGGCCTCATTTCGTAACCATTTTCAGCTGCATTCCGCACTTTCGGTGCATTTAGCCAGCTCGAGAGCCGCTTTACGGCGAAAAGGACGACTTTACGGCATTTATTTACCGCCCCACGACGACGGCCTGAAAATCCTTGACCTAAATTAATCGGTTACATATTTTTTTTTTTTCTGTCCGAGCTGGCTCAACTTTCGCAGCAGCATCCCCGCCCGCCCTGCCTGCCGGACGCAATTTTTGATCCACCTCCCATCATACCTCCACCTCCGAGTTCTTGGGGCATTTCCCGATTAGGAGCTCCCTTCCGAAACCAAACCCAAATCTCGAGTATATAGGCACACGTCCGCAGACATGTGAATATATACACCGCAGCGGCCACGCCTTTTGGGTTATATAATCCCAATGCGTGGAGCTCCGTCTGAAGAAGGACCATGCCCACTTAGTTCTTAGTTCTTGACCACGTTCTCCTCTAGTGTTACCTAATTTTAAGTGAACATAGTGCAATCTAAAGCAAGGGTTTGAAACCAAACAACCAACGTTAACATGCAAAGTAACAGTGCAAAAGATGATTCGGATGAAATGGAAATAATACCATCCGAACACAATGGTACAGTTCACGAAACCAAAAATGAAGGTATGCAGGGGATAATATCAATTATATAGTATATATGTACATATATAGTGTTCACTACTAGTGCTTAGTAGCTTAATTACATAACGTAAGATATCCGAACTCTATTCAATCCTGTGCATCCACAGACTCAGGTG

GAAGTCCAGCATCGACCCAAATCTATATCTGTACGACGATGATTTGGAGACCAGGCCCCATATATCAACGTTCATTTCATCAATTGCCAATTATGAGAACACGATACCAGCGGCCACCGATCCCGATGCAAAGCCGGCAGCTCCATCGGCTCGAATGGGTATGTTCGCCGCGCATTAATTAACTTGCTTGTTCCATCTACCCCCATATTGAAAGGCATGTGCACAATGGAATCGCAGCAAACAACAAAGCTGTGGAGGAATGATTGCTGTAATCCCCTCAAAGTGTTTCAGCCTTCACTGTGCGGGCGAATATTATAGAGCTGTTCGAAAGTATGCAACAGTGTGCAGTAGTGTGGCACAATCGAAGGGAATCGCTCCCGCGATTGAGTGCATACTTTCGGGCACCTGCCTAAATGGTTTCATTAAACTGATGACCCCAAGCATCAATCTCTAACGCTCGTTTCAATGTCTCATAAGGTACCCTAATTGGAGTGTTCTTGCCATGCATTCAAAACATCTTTGGTGTCATATTGTTCATTCGGTTAACATGGGTTGTTGGAACGGCTGGCGCCGTGTGTGGATTCCTGATTGTTCTGACCTGCTGCTGTGTGGTAAGAGCGTCCTGCTGCTGAGCCCTCCCCCATTTGGCCTCACTCCAAACTCCTCTTTGCAGACCATGCTAACCGCGATCTCGATGTCGGCCATTGCAACGAACGGGGTGGTTCCGGCGGGCGGGAGTTACTTCATGATATCACGGTAATTACAATGGCATTGGTAGGAGGTAGTAGTAACTCTAATGGTGCCACGATTAACATCAACAGATCCCTGGGCCCTGAATTCGGCGGAGCGGTGGGAATGTTGTTCT

 

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