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CRIMIC CR01079

Gene CCAP-R

CG33344

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	3R:25939786..25939764	96F8		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GGTATTAGGTGGCAGGGGATACCTTT	ATGTGCGGCTGCATAATATGCCATCG

Images

Construct Design Sequences

sgRNA sequence	GACTCGGAACCACAGTGAAGAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CGTCTAATGCCCCCAGATCC	GATTTTAGGAGCCGCAGCAC	CACTGTGGTTCCGAGTCCTG	GCCAAATGCCTTGCGTTGTA

 

Amplicon 5'

Amplicon 3'

CGTCTAATGCCCCCAGATCCGCATTCTCGTTTATTGGACAGCAATGCGCAGTTGGCCATGGTCAACAGTGTGACATTGGCCAACCTTCCAGCTGACCCATCCCCATGCACTTGTAATTGGCATATCTGGGCCAAGCGGACTTAATGTCCGAACCCTCAACTTTAAGGTGAGATTTCGAATTCACTGATCAACTTAAACATGATTTAATAGTGTTAACAGGGGATTTCGTGAGACTTTGGCAATGGGAAAAACCAATCACAATTGCGCCACGGATGACCGCTGGTCAGCCCAAATCCCTAACTAAGCCATTTGAATTGCAAATCATATGAGACCCCTTTACGCCTGGGATCCAGCACCCAAAAAAAAAAAACATACAAACATTCGGGATTCTCACGGCATTAAGAAAAATGTTGCCAGTTCAGCGACCTTTTTATGCCACCGCTTTTATCTTCACATTTTTTTCTGACGCCTTCTGTGTGACAGATTACCAAAAGCAACTTTCGTTTTGAACTGAATCAGACCCATGTAAAATATTTTTTATTTATTTTTCATTTGCTGCGAGTTGTCAAAGGCTTGTTAATAAGGGTAAAACTAAATAGAGTCGAAGAATACATACATATATTTTTGGTTATTTTATTTAGACCGGATTGTTTCAGACAAATTCTATCTAGGCATCGAGAATGAACCACATTAAAATAATCCTCAAATACAACTTTGAAAGTTCATACTATTTGATTAAATTAGGAATATTATAATAAATTGTTAACATGTAGGCAGGATAATATTTATGTTATGGATTATGAACGAACATACTACACAACTGTTTACTTAGAATACAGCTGTATAGTACCAAAATATGAATATAATATTTTTATTACCAGAAACCAACTATGCAAACAGCGAATCAATAAGACTTAGTTGCCGTGATGTTATTGTGACATCGGTTGCTCAGGCGGGGAGACAAAACCCTTAACATCGCTCTTTGTCAACCAAGATGCCATATGGTGCTGCGGCTCCTAAAATC

CACTGTGGTTCCGAGTCCTGCAAAAGGACTCATTTAAATAAGCCCGAATTTATGGCACGCACATACGCACGCACACACACACACGCACACTCACACTTTGAGTGATTGGCGCGTTTAAACTTGGCTGACTGGAAATTGGCTCGGCTGAGGGATACTTCCGTTCCAACAATGGTGTGATTTATACGATGGTGCGTATGGAAATTTCATTACATTCATGGTGTGCTCTTTATGGCATTCTTTAGCGGGGGAGAGAGGCAGAAGGACGAACGGAAGTTTAGTTTAATCGAAGTTGTTGTCACGATCTGCTGGACCCTCTGATTGATTGCCCCTCATTGATAAAGAGTGTTCTGTTACCAAGCCACGCTCAAAAAAATAAAAAAAAAATATGCAAATCCTTCACGCCGGCAGAAACTTTCGCCTCTTCTTGGGTTTTCTTAATTTCAAACTGCCAAATGACATTTGGCGTTTTATTGTTTTTTCTGCGACTTGGCTGGTTGACTTGTCAGCCTGAAATGAAGTTTGCCCAGTTCGTTGCTTTTCCATTGCGAAAAGGGAAAATTGTAGCAAATTGAAAATTGTACCGTCAAGCAACAAGCAGCGTCGGCAGCTGTGACTTTGTGACCCTTTCCACGCAGTTATCCTTTGTTTGAATTCGGCTAGGAACTACAGAGAAAAATGCTGCAGCTTCTGGGAAAAATAAGTATTTTATTTTTTTTGCTCTCCAAAGATCCTTTCTGCTTTTGCATTATATAGAAAACATTTTTTGCCAGCATTTAATGGTTTTTATATTGTACATATAACACTAAAATATAAGGAGTAATGTAGATACATTTTGTTTGCAGTGAGTCGATTAAGATGGAAATCAAATCGCACAGCTGCGCCTTTCATACACAATAAATAAATCGCATAAATGGCCAATACTCTTTCTATTTTTTCGCACCCACCTCATAAAAGTGAATCGAATTGAAATTGTTTATTTTTAAATTGATAGGAAGTAAACTTGTCCGAAGTGTGAAAATCGGTATTTCTTAGTAAATGTGCAGCACTTATTACAACGCAAGGCATTTGGC

 

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