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CRIMIC CR01083

Gene CG13743

CG13743

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2R:9057113..9057142	45A1	FBti0202669	81169

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	ATCTGGTCAAGGGGATTTCACTGGGA	GACCACATTATCCTCGAGAGTGTAGG

Images

Construct Design Sequences

sgRNA sequence	CTCCTGGGCCTCCCTCCCAATGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
AAAGCAATTTGTACCGCCCG	TGTGTGCGATGGATGTGTCT	AGATGGACTAAGCGGGGTCT	GTTTTCAGCACCCGTGGAAC

 

Amplicon 5'

Amplicon 3'

AAAGCAATTTGTACCGCCCGAAAGACCCCGTAGAAGGTCGCGGCCAGAATGCCAGAATGCTAGAATGCCAGATGTGTCCCACCCACACCATCCGTCTCTCTCTCTCTCTCTCTCTCTTTCCCCACTCGCCGTCTCTTTCGCACCGAGCTAGTGTAGGCGTTACTCGGGGCGGGAATTTAATGAATGTGAAGGGGACTTGAGTGTGAACACGACTACGATTCGCAATGCAAGTGCAGTCGAGAATGTGGGTCAGAGTTTACCTTCATTATATTGTTGAAGTCATCGAATGCATAAGCCTCGGCAACCGACACATCGCTGCCCTGGTGGATCGCATGAAAATCAAATTGTTAGCCATTAAATCGAGTTCACTTCAACGACAATCCGAAGTGCGCGCTGAATGATTCGCTTAAAACATAAACGTAAAATACACATCAAACCAAACACAACCAAACCAAGCACACATGCATGCACGAATCATACCGCACAAAAAAATTCCAATTTATTCGCATGCCAAATATCTGTATATGGATATACTACATGTGCATATATATACATATATATATATATATATAGCGCACATATCTTTATGGAACACGTGTTTTCACATATCGCTGTGGGAAATGCGAGCCCGAGCTCAGTGGTTGTCTGAGTTTTCCTCGTCTTTTTATCGTTTTGCCCGTCAATATCCGAGATACGAACGCGCACAGTGTTTGCTTTTTATTGTTCCGCCAAAGCAGCGGGGAGTTGAGAGTCCGCCAGGCGTCGAAATCGAATCCATTTAAGACACATCCATCGCACACA

AGATGGACTAAGCGGGGTCTCGATGGGGTTTTGGCGATTCACCTTGATTGCCTGCGAGCCACAATGGCTGAGGAGCAGCACTGGGCTATGCAACAGTTATTGCATAACTTGTGCGGGGTGGCGTATGCGTGATGCCCGTTGTCCATACGCCGTGTGAGCCCAGCCCACCGTCTTTTCTACAACTCTCCAAATATCCAGTGATTGACCATCAGTAGTCATTGCTTTTGAAATTTATCTAATTATGGTTTTCCGCACCACGATTTCTCATCACACCCGCACAATGTTTAATTTAATTGGGTCAATTGAGAGTGGGATTGAGTTAAGCGTTCGAGCGAATGGCTTCCGGGCCAATTTGAAATTCCAATGCAGACATAATTGTCGGCCCAGAGTGTCGGAGAACTCGTAGGGCTCCCCAGGGCCAAGTGCTGCACTGCCAGCTGCAGCTGACATTGATTTCTGTTATCTCACGAGGGGGTGACATTCTTATTGGATGCCTCAGCTGGCCCAGCTGCCACGAAGGGAGTAGCTCAAGGATCAGAAGAAGGAGGAGGCACAGGCGGAGCCGGTCCAAGGCCAGATGCCAGATGCGAGGCGCTGCCACCACATGCCGTGCATACTAAGCGGCGATTAAGTGGATGCGGATGCGGCTGTGCCCGGAGCAGGACCCATAAATCCATCGCCCCCTTATCCTTATCGCTATGTGTGGGTGCTGTGTAATTAAAGGCCGTTCGCGCCGCATATTGATTTTGTTATCTCGAGCATTTTTGGCGGCCACCTCCTTGGGGTGCCTAATTAAATCTTGGGGGATCTCGGACAAACTTTCACGCCCGTCAAAGGTCAGCGGTTAGACAATAAATAGCCGAGTCCTTGGGGCCACAGGCGATTCAATTGCTTGTCATCAATCATAGCATGTTGTGAATTGTGCTTTGGATAATCACTCAATCAGGACACGATTCGGTAGTTTCCATGTATCATTGTCTAAGTCTCGGTCGGATGGGAATGGGAATGGGAATGGGGATGGGGATGCGGTTCCACGGGTGCTGAAAAC

 

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