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CRIMIC CR01088

Gene Octbeta2R

CG33976

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	3R:12560102..12560111	87C1		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TTGACGTTGGCAGCGTCTGAAACTGA	GCGAGCTGACCACGCTGAAAAAGTTT

Images

Construct Design Sequences

sgRNA sequence	AGCATGAGACCCGAGCACGAAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TCCTTTGACCGAAGTGCCTC	CTCGGGTCTCATGCTGACTC	ACGAGCAATGACAGGACGAG	GTCGTGATTTCCCAATGGCG

 

Amplicon 5'

Amplicon 3'

TCCTTTGACCGAAGTGCCTCAAAGCGTTCTTTTGCTTAGGTTTATAATCAGAGCACGGAAATTATCTTGATGATCATCCATCGGCATCATCCTAAAATCCAGCAACACGCAAGTCACTTAAAAAATAAAACGCTTCTCATTAGCAGCAAGGGCAAAAAAGGGGAATTAAGCGAGGTTCAACCTTAATTATGCCTCGTCCCCCGCCCTAACTTTTATTATAATTTTTTGCTGCCTCGTTTTTCTGTGCCGCCTTGCAAAGTTTGTTTGTTTCGCCTTTTGGCTTCATTGCCCTTCGCCAACTTCGGCTAAGAGCATTTAAAGTTTTTCCTCTGATGGGTAGAAAAATTCTGCGTCTAAAGCTGAGCAGCGAAAAAATCGAGGAAAAATAACCGAGCGTTTGTCAGGCAGAAAATAACTTTTGATTGCGTCGGCTTTATGAAGCTAAATTGATTTGCCCGCCAGTTATTAAGGCATAACTAAAAATATTAACGAACCCCAGCCAGCTGATGTCCTATCATTTATCACTAGAAAAGTTTTCCTTTTTTCTTCTTTTTCGTATAACGTTATTTTCGTCGAAGGATAACGCCCCAAAAAACTCCGCCCATCATTAGCAAAACAAGGTCGCAGCAAGAAATTGACAAGTTCTGGATAGAAACGGATATGAAAAATAGCCTGGATTCTTTGTTAAATAATATAAAGTAATAAATTCCAAAACTAAAACAAAATTTTTGGATTGAATATAAAGCGTTTACGGCCTGTTAGGTTTCCATTTAAATTTTGCATTTAAATTCAACCGTTACGTTGATGGAGAATGAATTTCGTCAAATTAAATCATTATCCAGGCGTCCAATTAAGAATCTCTTAATATGCAAATTGGATTCATGAATAAAGTCATGAAAGTTAATTTGTCATTAAAATAATGCATCTCTCTGTTCTTTTGTTGTGGCCGCCTCCTTTCGCTAAATGGTGGGAGTAAATACTGCAGTCTCATGCCGAAACGAAGCGAAACGACAGGATAGAACAAAGCTGAGATGCTCAATTATGAGGCGAGTAAATAAAGGACACGGAGTCAGCATGAGACCCGAG

ACGAGCAATGACAGGACGAGGCGGGCGAGTAAAAGAAGGTCCTGCAGCACGTTTTGAGGGAGAAAATGGAAGGGATACAAATGGACGTGCATGAATTTTATGCAAAGTTGACGAAAATCGCGCATAGAAAGCCAAGCGCTTTGTAATTTTTTTCTCTTTCGTAACTTCTGCCAAATTGGCAATCAAGTTTTATGCAAATTACGAAAGAATTACACGGAGTGCGCGTACTTCTAATTGCTTTTTCACAGCTAATTAGCAGGAGGAGCAGCTGAAATATCACGTATACGCACCTTAATTTTCTAACACGCATCACAGAAATAATAACAAGCAGATTGCCGCAGATGGCCGCAATGATGATGAGCAGCATGACAAAGGCCTTGAACACCCAAACGATGTTGTCCAGCCAATCCTCGTCGGGATTGTGTTGTTGATGATGATGATGGTCACTTAATCCTGCGCCGCTTTCGTTGAAGTTCAGACCCGAGCCGCCAAACGGCTGTGTGGCATTCAGTTCGTGTGTGGCAGGTGTGGCAGTGCCCAGCGGCAGTGCTATTCCGCCGGTGGAAGAGTTGGATGCGCTGGATGAGCCGGTGGCAGCCAGGGCATTCGTTGTGTGCAGCATGGCCGCCGTGGTCAGTGCGGCCGCCGTGACCCGCGCCAATGTTGTCAGACTTTGGCCATAACTAATTGGGTTGCTAACTATCCCGCCGGGCTGCTCCGCCTGATTGCCGCTGCCGCCATCGCCGCAGCAGCATTTCGGTCTTTTTCTAATCCTGGCGGCACTGGTTCGATTTCGATGCCTTTGCCGCGGTGGCTCCGGGCCAAGTCCGTCGCACAACAACATGAGTCTCGTTGTCTGGTTTTAATTTTACAATAATTTATAACTTATCTTTGGCTTGTAGCGCTCTCTTTCTATCTCTTTCTCTATCTTTTTAGTAACACTGTGTGGTAGATCCGTATTTTATTATCGCACTTTTAGGTTGAAACACCCACTGGAGGAAGCGCCATTGGGAAATCACGAC

 

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