• About
• Search
• MiMIC
  • Technique
  • Constructs
  • RMCE Lines
• Transposons
  • GDP
  • Others
• CRIMIC
  • Technique
  • Constructs
• Order
• Downloads

CRIMIC CR01089

Gene gb

CG6070

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	3R:27110913..27110926	97E2	FBti0202670	81170

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CCTTTGAGCAACGTTGTGCCAATTCC	AATTGAGGAGCGCAGCTCACCAGAAA

Images

Construct Design Sequences

sgRNA sequence	ATGGCTTAACCTTGAAAAACCGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TATGGCCACCATGAGGGGTA	AGGTTAAGCCATGGGTTGTGT	TGCATCCACACCAATCCGAA	ATATATAGCCGCCATGCCCG

 

Amplicon 5'

Amplicon 3'

TATGGCCACCATGAGGGGTATGATCAGTGAAAACACGCCGAGAATCTTATCGCCAAAGGTCTGTGAAGAGAAGTGATCAATTAAAATCATTTGAAAATCCACTGGTTTAATTGACTTTTGCGATTCCGATCAAATAAATTAAAATTCCCAGCAGTCTATCGCAATTTTGCGAATTTAAGCTACAAATAGACAAAGTCGGACATTATAGGGCTTGCATTATGCTTGATAACTAACAGATTTTAAGCTTTTAAGTAAATTAGTAATTTGACTGCCTAATCTGATCGGACTGCAAATCGTGGATAAACTCTTACCACAGCGATGGCATTGGATGCGATCATCTCCGAGGGCGACAGCACGGCCAGATAAGCCACATTGGCCAGCACATAAATGCCGGTGACCAGAGGTAGGGAGATGTAAATGGCGCGCGGCAGGTTGCGATATGGATCGCGGAGCTCCTCCGTCATGAAGTTCAAGTAATTCCATCCGGCATAGGAAAAGATACCGGAGTAAAAGGCTACCGACATCTTGCCGGGATCAGTCTCAGTGTTATCAAAGGGTCTCGTGAAGTTCTCCACGTTGCCCATCATCATCCAGACCAGGCCTACCAGGATGATCAAGACCAGGGCGGCGATCTTGGTGAACATGATGACGTTTTGCATCTTGGTGGTCACCTTCATGTAGTACGAATTCAGGTAGGTTAGGAAGCAGATGGTGATGGCTGCCAGGAGCTGTAACGCTATTTTGGGAATCTCACAGGCTCCGCCGAAGAAGGGTTCCAGCACGTAGGAGGCAAAGGTCAGGCCCATAATGGCATTGGTCGTTGGTCTGTCAGAGATAAGAGCACACAATCAGTGGAAATTTTTTTGCAGACTGCATTCAGACATATGTCCTGCTGATAATGTCCGCATGCAAAAGTAGAAAAGCAGATATTTTCGCTTATCGCCGATCCTAAACAACAAATATAGCAGGCAATAAAACGCTCAATGCAGAACTCGCGGAACTGTTCAATAAATAAACTTATATAGCGCAGATTAAATGATAAGGCTCGTTTCACTTTCTAATGCTCTAATACACAACCCATGGCTTAACCT

TGCATCCACACCAATCCGAAGTATTCCATTCTTCTAGTCTTATCTGAGAAGTTATTAAACTTGACCCAGTCTAAAAATCAGATTCTGTAGGACTACTTCACTTCATTCAATCTACATACATATGTTATTTCGTTAAAAAAAACGTAAAAACGCAAAAAAGAAAAAAAAATGTCAGCTATTACTTTTACTCTAATGGGTTCCTTCTGGCGATAATAAAGTAATTCGAAATCAATTACCGAAAGGAATTTGATACTGTATTTATGTTTTTCGAACCAACTGTGATAAGGGAACTTTAACGGCGAAATATTCGCGAATATTAAAACAATATTGACCAGTAAGACTCACACGAATATCATCATGGCATCCCAGAGATACAGGAAAGCCGGCAGTGATCCGTAGGCCTCGAAGATATACGCGTAATCCCCCCCAGATTTTGGTATGGCAGTACCAAGTTCCGCGTAGCAAAGAGCACCAATCATGGACAGCAGTCCGCAGAGAACCCAGATGACCAGCGAAGCGCCCACGGACTCCACCTCCCGGATGACGCCTTTCGGAGAAACAAAGATACCGGATCCGAAGATGATGCCCAGGATGATGGCCACTCCTTCGAGGAGGCCCAGCTGCTTCTTGATCACAACCCTGCTGCTGTCTGACTCGGCGGATCCCTCGCGACACTGAGCTTTCTCGGCAGGATTAGCCGCTGGTTGGGCAATGCTGGAGGGAGGATTGCCGGAGCTGGGGGTCAGCAGGACCATTTCGCTGGACGGCGACTGCAGTTCATCGGCGGCGGGCATGGCGGCTATATAT

 

---

Search Again