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CRIMIC CR01093

Gene Traf4

CG3048

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2L:4373409..4373433	24E1	FBti0202671	81171

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TGCCCAAGCAAAGCAATCAAAGACCC	CGGTGCGAAATGTGTCATTACCAGCA

Images

Construct Design Sequences

sgRNA sequence	GGATACTGACAGTCCCATCTTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
AGTCGGGGCTCCCTAGAAAT	GACTGTCAGTATCCCTGCACA	GCACAACGTGGACCGATTTC	CAGGAATGCCAGTCCAGGAG

 

Amplicon 5'

Amplicon 3'

AGTCGGGGCTCCCTAGAAATATCGAGGGCAATGCACCAGAGATGTGATCTTTTCGTCTTGCAGATTACATTACGTGCTAATGCTGCAAATTTCATATCTTTGGTGTATGGCGTACTATACGCAAAATTAGTCATAGGACAAAGGCTATTATTGTTTCTGAATCATAGGATAACAAAAGCTTAATATAAACAATAGATAGTTTGGAGAAACTCAGTTAAATTCATTAATGATATTCAGATGTGCGTAAACAGAGATTACAAACAGGGAACGCATCCGTACGCCTGTGGCGGGGACTTCCTGTTTTGGCGAGCCAGCTGTGCAAACATTTAACAACTAAAGCCCGCATATAATTGCCACAGCCATAAAAGCGACACGAGTGCATTCCATTCTACTCCATTCCCGATAAAGCTCATTGACCCAATTGAATGGGGAGCCCTAGACCAGCGGCCAGATACTAGGGCTTAGCCGCCACTTATCATTATCAATCAATCAGTTTCGTAAAGCAAATAGGGCCATCTAGAGCGTGGGGACGTCCACTGATAGCGGATTTCCACACCCGATAAGCTGGCTGACAACTATCGAATATACATATATATTTCATATACATGTATGTATGGATTTTCCGCACCAAATCGGGTGCAATTAAGCCCCCACCGTTTGACGGCCAAGTTGAGAGCAATTTGCCAAAGCTGTGCGACAGATTGCCCGGCCTTACACACATGGCAGGAAAATGGAACTAAAAAAAAATTCCAAAAGAGATTGAAATAAAAATCCGAAAGGCAAACAGGATGTAGACGCGCTTGAACAACTGAGGATGCGATGGGAATTTTTTGTGCAGGGATACTGACAGTC

GCACAACGTGGACCGATTTCTAGAAATGGAAAGAAGAGCCGCATCGAATGGGGAAATAGGGTTTATTGCGCGGCATAAACAAACTGCAGTTTGGAGGAAAATTAAATTTAACTAGTTAACTGTTCTATGAGCCATCGATTACCTAACAATAAGGTGATAGTTCCAATTAGCCTAAACTCAATCTACTTTTTCTGCCGCCCAAGACACATTTTCCCACCGACTAAGCCCATTTCAATGCGGATTCGAGTCTGCAAAGTAATCAGAATTCCCGTACTTCGATTTCGCTTGCACTTTTTCGCAATCCGCTTCTGACATTCTCTCCCTGGTCAGTGGATTCCGCAGACGAGTGCTGCATTCATTTGCAGACCCAGACCCACTGCGAGCCACACCCACGCGGCACGACCAAGTGGCGCTGCTTGGCTTAAAAAAAAAAAAATAAAAACATAAATAATGAAAATTCAGCAAGACGACGACGCCACGAGCATGCCACAGTTTTCATCGGCGGCCGGGAAATGGATAATCGTGACCAGTGGCGACGAGGCGCCGCCGACGCATCCGACGCGGCAAATGCCAAAGTTGAAGAGCACTCAAGATACGGGAGTCCAACGCAACCGTTAGCCGAAGGAAAAGCCAGGTGGACCTGGGCTTATGTAAGGCAGGTTCGCATTTTTGCCAACAGAAACAATTCAGAAAACGAGTTTCTTGTTTTGGTTTTGGTCTTAGACTAGCGAATCGGTCGGAAAAGTTGCCCAGCCGCTTTTCAGCTGCTGCTGCAGTGCTGCGTCGGTGCAAAGTACAAACATCCTTGGCCAAAGGTAAACCAGTTAACCAGTTCCAGTTGCCAACTGCTGACGGGGGCGTCTGCCCTGTTTTTTTTTTACCTCCTCCTGGACTGGCATTCCTG

 

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