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CRIMIC CR01096

Gene NimA

CG42282

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2L:13959138..13959141	34E5		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AAACTGCTCCTCCTGCTTGCAATGGT	TGTAAGTCATCATTCCGCCCCCAAGA

Images

Construct Design Sequences

sgRNA sequence	CCGAGCCTGATTAGACCCCTCGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
ACATCTGCATACGCGAGGAG	CTCGGATCGAGCAACTGCA	GTCTAATCAGGCTCGGCTGG	CCGCATGGCCACCCTATAAT

 

Amplicon 5'

Amplicon 3'

ACATCTGCATACGCGAGGAGCCATATGTGGAGCACGTCCAGGTGCCAGAGATGCAGCCGGTGCGTGTGCGGACCTCCAGTTGGTGCATGGAGATCCCGCCGAGATGCGCCACCTTCAAGACGGAAATGCGCGAGGTGATGAGGGTCCAGGTGAGTGAGTAGAGTAGTGAACTGATTTTGCCTCCGGATCAGGGAGCCTGGCAACTGGCCAAGTATCCAAATGGACGCAGGTGTTCCACTGCCGGCTCTGCCTTTGCGGGCATCTCTTGCGTCACCGCGATCCCAGCCAGTGGCAACACCTTCGCCGTGGCCACTTTTTCCACCATCCCGGGGAGCCTGCGCCACAAACCTGATTTCGAATTCGATTTGCCCAACCAGGAAGCCGCTCGCCACGATATCCATAAATTGCCCCGATTCGATGGCCGACTTTGATTACATTTCTCATATATATCGCTCGCTATATGGGTTCTGTCCGCCACCAGATGCATTGCGCAATTCTGGCCAATCTAAACGGGGTTTCCTGTCCTTCTCGTATGGAGAACGGAAGCCCCATGGATTGGATTTAGCGGCAGCTGTGCTTAACTGCAGGAGCTGCCGTTGCACTTTACTTGGTCAAGATGCAAGCGACCGGTTTTGACCTGTAGCATCTGAGGTGCCACGTGCGTCCTTGGCTGCCAAACTTCTTGAACTACTGGACACATGTGGCGTATACGCATTATGTATAAAGTGGTTCAAAGGTATTGTTAATATAATTATTGTTATGAAACAAATATTTAGAAATTTGAAAAGCAAAGTGTTTAATAGTGGTTATCAAATAGCAAACATCGTCAATTATTAATGTTTATTTAAATGGTCATTAATTACATCTTAAAAAATAAATAAAAGAACCAGCTCGCTTACATCACAATTTTGCGAATGTTTTTGTGATAAATGATGATAACTGATTCCCTATTAGCGTCAATCAATAGTTATTAATTTTAGGCAATTGTTTGGAGCAGACAAACAAACTATTAAATCCACAAAAACCACCGCCTGGCCATTTTCAACTGCGCAGTGGGAAAAGCGAGGAAAACAACGGCGGCGGCCAAGTGTTGACCAAGCATGGCCAGAATCCGGTTGCAGTTGCTCGATCCGAG

GTCTAATCAGGCTCGGCTGGTTTATTTTATTATTTTCTATTCATTTATTTACTTTGCGCACTTTTTCGGTGGATACAGCGGGCGAATACGGGGGGGTTTGGAAAATAATTATGTCATGGTGTCAGTGATGGAAAGTGCAAATGAAAAGCGGAAAATCCAGCACTTATGGCCTGGCTGGATAGCCAGGCGGACCGATCTGCAATTGGGCATTAAGAGCTCCACTTGTATGCGGTGACTTGATGCCAAAGCTGTTGACACACTTTGGATTGCATCACTAAGCCGAGTTGCAAGCCATGACAAGAAGCAGCCAGTTTTGGCTTAGTTTATTTATTTTTTTTCCTCGGTTTCGTGTCTAATGATTTGTGAGATTTGAGATCGCTTGCGCTTGGCTCGAATTAATTTCATAATTATTTTAATTGGAGCTCTTCAGGTGTTTGAAGAGAATCGCCTTAACGGCCAGCACCTCAAAAAGCATTTTTCTTCGATTGACGCAACAATTTTTTTTAACCCTGTCGCTTGCATTCCTGGCGATTTTGGTCAACGCCTGCTTGGCCAACACTCCGATTTTGGTTAGAAAATGTAGCCACTAATTTGCCAATTTAATTTAATTAATTAACATCGAGGTGTACGCCTTAAGAAGTTCGAGTGTAAGTGGAAAAATAAATTTGATCTCTGGACTGGCTGTAACTTGAGCCAGCAAAGTGCCGCACGATTGTCGATAGCTTTCCAAAGGAGAGATGTGATTACAGCTGTGATTGATGGGCAACCACATTTCCACTGGCAAGGGGTGTAGTATTATAGGGTGGCCATGCGG

 

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