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CRIMIC CR01101

Gene Tsp42Ea

CG18817

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2R:6994533..6994551	42E4	FBti0202672	81172

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GCTTTTCCCGATCTTCACTAGCAGCA	CGTGTTTCCATTCCATGATGTGCGTG

Images

Construct Design Sequences

sgRNA sequence	CTGAGAAGTCGATCTAAGCTTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
AGACCACAGCGGAAAAGGAG	GCTTGGCCGCTCGAAATTAT	CCTTTTAGGCTGGCAGTGAGA	TTCCGCCCGTAGTGAATGAC

 

Amplicon 5'

Amplicon 3'

AGACCACAGCGGAAAAGGAGGCCTGGCTCTCCTGGGCCGCCGGCTATCCCTACATCTGGCGCCGCTACAACGAACTGAGGCAGCGCTGGTTGCCCAAGGAGAAGCTGCTGCCGGAGCTAGTGCGCTGATTGCGATCTCCCCGTTCCTGCCTCGATGCTATTTACAGGGCTACCTAGCTCGCCACCATTAACTTAGTTCCATGGCCAGCTACTCTCTATATTTTTATTATGCCTGTTTACTAGCTTGTGCATTTGTGTCTTAGTGTTGCTTGCTATCTATTGTTATACGATTGGCAAATATCATATACGATATAAGACAGCATTTGTTTACAAACCCTCGAGCACAAGTACACAAGTCAGTCTCAAAAGCTTATTAGTAAGGGGAATAGCTAGCGCGACTTGCCCAACGCGACTTTCGATGCCCAGGCGGTACGTGATCGTGTGCTAATTTCCACACCCAGTAGAGCCAAGAACGTCAGCATTTTTTAAATATTCAAATAAGCGAAATGTTTTTACCACGGATGACATCAGTGCGTGTGCCTGTGTGTGTACGTGTGCGTGTTTCCCAAATAAAATGCCGAAATACCTAACTTTTCACGCTTGTATCTGAATTTCTTAATTCCATTCCATTTTTTTCGTCTCCCGAACGATCAGTTGCAGTTCTTCATCCATCTCCACCAGCAATCTCTGGCAAAACTCAGGCAAAATGAGCTGCGGAATCTCCATGGTTAAATATATCCTATTTATATTTAATTTGCTCTGTTCGGTGAGTAGCATAGATACATTTAAGATTACATAATTTCGAGCGGCCAAGC

CCTTTTAGGCTGGCAGTGAGATCATAACTTTCGTGCGGAATAAACTGGTTTTCCCAAAATTGGTGTTTTCAGTATTGAAAGGCTGAGAAAGTCGAGGCCCACAAAAGTGGTGAGCATGAGTACATTTTAAAGCCACAGAAGGCACTCTCGTTAAATGCATTTTTGTATTTTTGTGTTCTTTAGGTCGGCTTTCATATTTACATATCATGAAATTAAACATATCTCAATTAATGTTTATTAAGGACCCTTAAAGTAGGATCTATTATAGCATTTATGGTGTCTCTTTTCCGGTCAGCACTATCACTTTCCGGTTACGAAACGCACTGTACGTGCAAAGTGGGGGCAGAGCTTGAGATCATCATCAGTATTCCAAAACCAGTTATTAAAATTGGGCTACGTTTGTGACGTAGCGGTATCCGAAAAATTCTGTATAATTTTGTGTACACTACGTACACTTTTGTTTACTTTGGAGTCCCAAAACCAGTTTGTGTTGTTTCGGTCCCCACAACCGAGCCCCACATCCACAAACATGCATCCCATTAGTCGGAGTCAGTCCGAGGTTAACTGGTTCCAAATGGCAACCAAACAATCATTGAAACGCCAGCAACGGTGGCATTTCGATATGGTTTTGCAACTTCTAACTCACTGCCCCAAAGTCACTGCACCGCCAGGTGGAACTGTACTTCGTCCAACCAGTTTGGGGCTGGGACCCTGACTTGCTGCTGGTGGGAAATCTGACTGCGTCGGAGTACCAGCGCATTATTCGCGTCACGCAAACATGGATGTCATTCACTACGGGCGGAA

 

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