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CRIMIC CR01104

Gene CG33958

CG33958

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2R:18191270..18191280	55B12	FBti0202674	81174

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CCGGATATAGCACATAAAGCCCCTCA	TCAATTCACTTTGGTCTCCACCGCCT

Images

Construct Design Sequences

sgRNA sequence	TGTTCCCTAGGCACCTGTAGTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CCATGGCACTTACCTGAGCA	ACAGGTGCCTAGGGAACAAC	CAGGGCCGACGGATAAACTC	ACTCCCACCCTTAACGCTTC

 

Amplicon 5'

Amplicon 3'

CCATGGCACTTACCTGAGCAAACAAACCATTTCAAGGTTTCGCTCGATGGTCAAGTGAACATTGAACAAGCTGGACATGGGTTCCCCCCTATCATCTACATACTTGGCGTTTGATTGAATCGTTGTAATAAATTATAATTACGCCTCAAAGACACAATTTCCGGGTCCGTCTTTCCCTTTGTTTGCTTCTTTTCATCGACGCAGTCACTCGGCCACTCGGAATCCAACCAACCTGACCCAACCACCTGCTCACCTGTGTCTCGATGTCGGCCACAGCGGAACGGTATTCCAGGATCTCTTGCAGTGTCACCGATGTTTGCACAATCAATGCCAGTATTGGTATAAATGGTAGTATTATCATCTGGAAATGGATTCGCATGTCAATGAGTAAATTTTCGCTTACAGTGGGCTTGAAAGTTCGAGATTTGGTAGTCTTAAATAATTGTATCAAACAAAAGGCAACTTTGAAAGTCGGTTTAAAGTAGACAATATAAATTATTTTTAAAATACCCTGTAAATAAGCATAACTTAAGTTAACTAGTTTCTTTGAATAGCAAATGCTTTAAATTAATTAGCAAAAATATTAAATGAAGATTTTTGCGCTGAACAAAAATCCTTTTTAATTCTGTAGATTACCAACTTCTTGCGAAACCTTAGAAATTGACTTTCCTACAAGGAGTTTGAAATAATTGTTCTAGCGCTCTTGGCCCACTGTGCCATATATTTTCATTTGGCATCAATATGTGCTCAGCGGAGAAAGAGTCCGGACCAATCTCGGCTTTATCTGAAACTGATACCGAGCGGTAGAGGCAATGCCAATGGAACAGCACAGTGGGTAAGGGCGGTGGTAAAGGCGGCAAAGGAGGCGTGATTAGTCTTTATCGTGGCCCGCTAATCGCGACATTCATTAGACGCACTCTGTGGTTTCCCAGGCACGTGACTCTGACCAGTCGTCCTAATTAAGATGCATACGCTGGCGCTAATTTATGCTGCTCCGGATAAGTTGTTCCCTAGGCACCTGT

CAGGGCCGACGGATAAACTCACCTGCATCAGCTGCAGTCGTCGACCCGTTTTTATAGCCGGATTACGGGCGGCGGCATTGCAACATCTTCCACTGCCCCCATTCGATATATCCTCGGAACTAATCGAGCGCACGGAAATTTTATCGAGCTGCAGATTGTGATGTGTGGGCACCAGGAGCAGGCCACCCTCCCCCGATGGATTCACGTCGTCGGGAGCCGTTCGCGATTCCATCGGACGGTTCGATTGGCAAGTGCTAATGTCGATCTTAACGCTGGGGGTCGGCATCCTGCTGCCGCCCGCTGGGCAAGGGCTAGGGTCAGGGTTCTCGCCGGACTTGTGCGCTTTTCCATTTAGCATATTGCTGATTGTTCAGGCGAGGATGTGTAGTGCCAGTGCCTCATAAACCCGATTGCTGCACTGGGAGAAAATGTTGCACATAAGGTCATTTTCTATGTTGCAGAAACTCCATTCTTGATCATATTTTAAATTATGACTATTTACAAATTCAGTGACAACTTAAACTGTGTACAAAAGTATTTTAAACCAAGAACAAAAAACTATAATAATTTAAATATTTGTATACTTCGTCACTTAAGTAACTGAACTAGAAGAAGATATGGGTAGGCTTTTTTTTTGCCAGTGCACTCGAAGAGCAAATGTCACTGGGACTGTACATAATTGTTGCGTGTTCAATTTGTATCTGATAGTTTCACTCCGGCGACACTTGTTGAGTTTCAATGGGGCCGTAATGAGGAGGGCTCGTAGAATAATTGCAAAGAATGGCATATAAGCACATATGGGTGGCCGGGCCACGCCCCCGCCCCGTTGCCGCCCATTCGCTGGCCGCCAAACGAATGTCAGGACGTCGAGACGTCAGGACGTCAGGACGGGACGGTGGACGACAAATTTGATGGATGAGCAGCCAAGCAACCAAGCGTTGCGGACCGAAGCGTTAAGGGTGGGAGT

 

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