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CRIMIC CR01109

Gene side

CG31062

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	3R:27415928..27415938	97F10	FBti0202675	81175

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GTAGCGAGTAGTGATGGAAAACCGGT	GGCCATTGAATGAGTGCCAACCTTTC

Images

Construct Design Sequences

sgRNA sequence	GTGGCGGTTTAGATCACAGGCGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TTTCCACTCTCGTGTCGGTG	CGTCACTAAGCGCACATTCG	ATCTAAACCGCCACGCCC	CAATATCTGATGCCGCCCCT

 

Amplicon 5'

Amplicon 3'

TTTCCACTCTCGTGTCGGTGCCGTCTGTCAAGCCAGTTGCTCCCCTTGGCAGTTTCCTCCCAGTTTTTCCAGCATTTTCAGCCCACACCCCAGCCATTTCCACCGCATACACGTACTGTGTGCAGTGTGACATGTGCTTCCTCTGTTGTATTTTTTGAGCTCCCTCCTACCGGTTTGTGTTCTCTTTTCGTATTTGGTGCCACTGTTATTTTCCACATGTGAAATTGAACTTTGCATCTTGCTGCGACGCGTCGCAACTTCAGAGATACAAAATGCAACGAAATTTTCATTTGCCAGCGCCGATTTTGCACTTGACTTGTGGCGCTTCTGCCGCCTGTGCACCACCGACAAGCATCTCCACCTCTGCATCCACATCCATATGCGCCACCTCCCTGTCCCCTCCCAAGCAATCCCCAGCCATTGGGAGCACTCCACATGGTGGGGCATCCATTCTGACCGCCTGGCAGCGCTTATGACGCTGTTTTGACACTGCGAACGCGCGCGTGTTTATAAATAAAATATTTATGGAAATATATATTTGTGAAATTTTTCCACGCTTCTTCGCTGGATGGATGGATGCTTGCTTGCTGCTTGTTTGCCGCACATGTGCTGCACCCTTCATCAGCATCTGCATCTTCTGCTGCTGCTGCTGCTGCTGCTGTTTTTTCCTTTGAAGTCCATAAAAATTAACAAAAAATCAATTTACAACACAAACGATGCGCTGATGAGGCGAGTGAAGAATGAGAAATGCATGGGTAACTTTTATATGTATGGTGTGGAGTGCCCTTGTGGATGTTTTCATGTTTTCATTGCAATCGAATGTGCGCTTAGTGACG

ATCTAAACCGCCACGCCCACTGCCCTTGGGGTGGTTTTTCTCCCAGTGCATGCATATGAACACTTTTTGCGAGCGTGTTGATTGCCCATCAAAGCAAAGCCAAGGCCAGGGAAAAAGCAGCAGGAGCACAGCAAGAGATTTGCATAGGGGACACACACACACACTCACACACACACAATAAATCCCCCTAATGCCAAAGCGCTTTAATACTTGTACACATAAAAAATTAATGAAAAATTTAAGAAAATATAAGAGTAGGCAACTTTCAAATCTGCTACACTATACCATACCAAAATGATAATATTTTCAAAATAACGACTAGATTGTGAGTTTGTGACTCACTATCAAGACCAATTAGAGTCTTTCGAAATCGAAATCTATTTTTTCCCCGTGCAGTTGTTGCTGTAGCGTCAGCAGGAACATAAAAATAGCGACGATTATGATGAAAATATTCATGCGACAAACGGAAGCCATATCTCCTGGCTGGTTGGTTGGCTCTGGAACTGGTTCCCCCAAAACACGCGATGTGCCGGGACTTGTTTATGTGCCAACGATACGCAGAACATTTGGATTCCACTTAAGTCGTCACCACGCATCCAATGTGTGTGTGTGTTTTTGCCTTTAGCTGGATCAATAGCAAGTCAAGTCAATGGGAGTCGAAGCACTTGAGTACTGAAGCTATGGAAAAAAGAGGCTTTGAAGGCGTGTCCATGCAAATGCCAAGTTGAAACCGGAGTCAGACGAGGATTGGCGCTAATAAAGCGGCAAGCGTAATTAAATTTAATGCCCGGGTTGCTCAGACAAAAGCCAAACGTCCATGACGCCCAAGTCGGAAAATGTCTAAATCCGAGTATAGAAATTCAATTTACTACAAAATCCCATGCATCGGTGGGCTCGACGCTCGACGACGACAAACGGCAGCGTAATAGAAAATGCTGGAAAATGTGTCTCGATTTCGGCAAATCCTGTCATAAATTTCAGGCGCCTTTGGCCACGTCCCAGCCTAAACCAACCAGCCGCCCAACCCATCCAGTTCTGCGAAACAAGATCCTGTGCGGCAACAAAGGAAGGCGGCACGCAGACGACGAGCCCGAAAGGAAAATATGCTGATGTCGATGCCGAATCATGATTTGTTTTTGTCGACTGGCCGGCGGGCAGAAAGGGGCGGCATCAGATATTG

 

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