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CRIMIC CR01110

Gene CG10345

CG10345

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	3R:16600729..16600762	89D5	FBti0210323	86336

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TGTCTTGTGTGTTTTGGCCCGCCAAT	AAGGCATTGTTCTTACCGTGTCCAGG

Images

Construct Design Sequences

sgRNA sequence	GAAATCGGTCACAGGAGAATTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CAGTTTGTCGATAGCGTGCG	GCGCACTGGGTTAGCTGATT	CCAGAGATCATGGCCGCTC	GGGCCCTAAGTTCGGGAATT

 

Amplicon 5'

Amplicon 3'

CAGTTTGTCGATAGCGTGCGCCCCCCATCATCGTGTCCATCGTGCCATTGACCTTGGTGGCAGATCGAAGCGGATAGGGTCGGCCGAGATCCGCGACCTGCCTCAATGGCAGGAGCAGGAACAGCGGCTCCAGGCCAAAAATTTTAATAAACAAAACGATGACAACCGGCAGACGACAGCCGGCAGACGATGACGATGCCCGAATTCGGAGAATTGGCGGTTCAATGAACGTTCTTCGATTTTATAATCACAAATGCGTCGGAAGGCGATACATGCTCTCAAAAAAAGAAATCGAATTATAACACACCAAAAATGCCAATAATTCTAGTTAAGTGCCCTTGCTCTGGGACATTTTTTCCGGCAATTTTAATATATTGTCTAAATTTAATGGTCATATTATTCGTTTATAGACGGCATTAGCAAACACCTACAGCTACCAACAGGTTTTTTTTTTTGCACAGCCACCGACTTTTTGGCATAAGGGTAAACAAAAAATATTTGTTTAATTTATGGTTACACCTTAAATAGCTTATTTCCCACTGTCACTGGCTTGCTTTTAATCGTTTTTAAGGCAACGGAATTTACTAGGCTGAAAAGTAAATTGCGACTGATTTTGTGTTTTGTTTACACCAATTGGATAAGAAGGCCGCTCCTAGAAAAGTCGATGAAATCCAGTTCCACTGACCTTGTTTGGAATATTGATTAAAAGTACGGAAGATGGGCAAGGCAATCGAAGCATATCAATCTAGGCTGCAATTCTCCTCATATTCACTTCAAGTGAAATCACTCATTTTACGACCGAAACACGTTTGTTCTCACCTTGCGCTAAGATTATTGTCTTTGCCCCGGTGAAATTCACTTCCGCACTCGAAAAAATGGCCGGAAATTGAAAGGAAATCAGCTAACCCAGTGCGC

CCAGAGATCATGGCCGCTCCATGAAGAATCACATTGGGTATTGTAGGCGGTCATAAATCAAACTCACTATCGTAGCCCATTAATCGGTACAAATCGTTTAGCGAGTTCTCGCAACAAAAACAAAAGTAGAAAAGTCCCCAACTGTTTCTATGGAAGTAGAAGTAGTAGAAACCGAAAGCAGCGACCGATAGATACATAGATAGATAGGTAGATAGATAGGCGCACCTGCCAAGATCAATGAACTGGCTAGCACGCCCCTAACTCGCTCCAAAATGGCACGATCCCACAGCGTCTGCGATCCGCGGCTGAGATCGAAAAGCAAACAGCATAAACTCATTAACAATTTCGCTTTGGTGTCAACTTTGGTGTCATAAATAAGTTCAAGACTTCACAATCAAAACAAACCGCAATGATACATGACAAATTATGCATGGCTCCGATGAAACCAGAAATCCAAACATATTTCCCATTCATAGGAAATACGCATGTGCACAGGAAAAAACTACATTTCAATTAATATAATACAAAATATTATTTTTGGGCCAAAAACAATGACGTGTTCTTCATGGAAAAGGTTCATAAATACATCAAGTTTTTATATCTTGTGCTTATATGACAGTAAGAACAATGTATACTATCTGAAACTATGAACCTTTTCTCCACTCCTTTTGTCGTGTTAATTCCCAGAACTCGTTTTAAATATTGTGTGTACAAATATTGACAGAATATGTTCTTTAAAAGTGATGAGGTTTTGTGTTGTTAAATGTAACGATTTTTTTTTAAAGCCAGGTTGTCAGGTATATATGGGTCTTGCGATCTTTCCGTTCCCACTCAAAAATCAATTATGTGGCCTATATTTTCTCGCAGTGTGCGTTTAGATACTTATGCATATCCTTGCCTTGAATGTGACAGCCGAAATTCCCGAACTTAGGGCCC

 

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