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CRIMIC CR01112

Gene CG7432

CG7432

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	3R:19666550..19666585	92A13		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CGAAAAGCTAAAAAAACGTCGCCGCG	GCTAGTTATGAAACGCACTGTTGGGG

Images

Construct Design Sequences

sgRNA sequence	GTGACTGTTCTACCGGTTCGTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CGAAGGTGTTTCTGCTGTGC	CGCAATAGCAGAGCAATGGG	GCGTGGCAAAGCTCTTCTG	AGCTGGCCAAACTCACTTCA

 

Amplicon 5'

Amplicon 3'

CGAAGGTGTTTCTGCTGTGCCTGGGTTGGTATTGGAAAGAAAAACAACATATGATTGTCCCCAAAATTAACAAATAATTAACCTACTTTTGGGCATCCCAAGCATTTATTTGGTAACAATTTATCACCCTCTTATGGTTAATTTGTGCTAATGTGCTGCGCAAAATATATCAATGAAAAAACAAAAGACTTAGGCAACGAACAGGCTGTTGCGCATCCGACGAAAGTCCGAATGCAGCTGATGATTGCGGTTTGTTGTATATTTTTTTTCATTTGTGGCTATCATCATGATTCGTGCAACGTGAGGCAGCTTGCAACTTTCTTTGGCCGCCACTGCCAATAAAACTTCCGCTCGGTGACGTCACAGACAGGTCGAAAACCAGCTGGCATGCAAATCACATAGATAACGCCACCAAGGCCAATCGCATATCTCCCCTGGCCCAAAGAGTACACTTGTGTACGTGTTTCTTAGTATTACCGCAACGCCAGGCTTGACCATTTATTATGCAATAAACAAAATAAAAGAAAGAAAAAAGTTGTCAACGCCGCAATTTGTTGGCCGTTTGGTAAGTTTGGCCTGGCCATTGCCGGCTGCCAAAACTCCAGGCGGTTTGCCGATTGCCTGACCATGAAGTGTACAATTTTTGAGATTTCTGCTTCGGAAGTTAATGCAGCCGCTGTTTCTTTCGATTTTCCCCGTTTTGTCCCTCTGCGGGGATTAGCTTGTAATTTTCCATCAGCGGCTATTGCAGCGTTGACATTTCAACTGCTTTCTTCGGGATTTTCACTTTCGTTAGCCGACCGATGATAGAAAGAGTGTTAACTCGGAGCTGACAAAACTCCTAGCCTTGGTTAATTGGATTTATGATTTTGGGAGAATTGATTTAGTAATGTATAAAATATTTAGAAAAAAAACGATATTTAGTGCTAAAAACTCGGGTTCCTCGACTACCAGATACCCATTGCTCTGCTATTGCG

GCGTGGCAAAGCTCTTCTGTTGCACAAAGAAGAACATTATGCTTCCTTCCACCTGTTGCGTACTTAACAACGAATATAGTAAACTCTTTTAGTCAACGAGTAACGGGTATATTTACTTCTTTATTAAAATACAAATGCTGTCGTTTATATTGTAAACAAGAAATAAGGAAATAATTTTAAACAGAAATAGAATTAAGAATTTTCAATGTTATTTTCACTTATTTCTTTGAACGGTGTGACATGTTTTTGGGTCAAAATGCATAAAAGTATGATCAACAATGAACCAAAAAGAGCGCACGTATCCATAACGAATGTGCTTAGTCTGTCTAGGGCCTCCGGGGCATTCACTTTTCCCAAGAAATCATTTCTATATAAGCCCTATAATTGGCCAATATTTCATTGTGTTGAGGCGAGCAGCAACAAAGTGAAGCAGTGCCATTTGGTATGGGCAATTTTATTCCGAGTGAAAGTGGCAAAGTTGCAGTGCACACCTCGAAAAATGTTAATGAGCCGCGTTCGAATCTGGCATAAAAGCAAAATAAGAAATTCAAAATAAAGCAAAAAAAGGCGAAATAAAACCGAAACTATAGCAGCTTAACCCAAATCACTTTAAGATAATCAATTTTGTTTTCCTTCGCTGCGAGGTGAAATCTTTTGGCGACCTTGGCGCCCATTAACTTACATAAAGAATCATCCGGGAGGAAGTTCCTCATCGCGGAGATCTTCTCCTTCTAAAAATGCCAAGTCATTAGGGTAAATGGAGACAAGCCCACCAAAAAACCGAGCAAACCAAGCCCGACGAACTGAGATTATGTCACGGCAGTCAAGCCAAATAAACTCACAACTAAATAAACATTAAACGCTTCAGTTGAACAACGACATACTTTCCCATTAAGGGACAAGATGGGTGCGGGGGAGGGGGCGTACCGCAGATGCGTAATGCCAATTTGTGACATTACTCAACCGGCGACAAAGCCGAAAGACTTTGCATAGGACCATTGTTGAAGTGAGTTTGGCCAGCT

 

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