• About
• Search
• MiMIC
  • Technique
  • Constructs
  • RMCE Lines
• Transposons
  • GDP
  • Others
• CRIMIC
  • Technique
  • Constructs
• Order
• Downloads

CRIMIC CR01114

Gene plum

CG6490

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	3R:26508266..26508286	97B6	FBti0202677	81177

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GTCTCCTCTGTTGTTCGATACGATGG	GCCCAAAGCCCAAAGCCAGTTTAGTT

Images

Construct Design Sequences

sgRNA sequence	AACAGGGAGCGATACCTACCAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
AGGAGAGAAGAGCTGAGCCA	AGATCCAAAGCAGCTTCCCC	CCTGTTGCCCCTAACTCCTG	TCACCGCACAGATTAGTGGC

 

Amplicon 5'

Amplicon 3'

AGGAGAGAAGAGCTGAGCCATCACGCATACGTCCTGCTGCCCCTCATCGGATGAATATTTCACAATCGACTGTGACACCATGCACCGAGTGCAGCCCCTACCGAGTGGTCCGCTTAAAATGCAATTGGCAAATTACGGGCATGCCGGCAACTTGGCATTCAGCACGCGCTCGCACTTTATCATATCAATTTCAATTTGACTCGAATTAAGGAGGAAACGACAGGGACAAAAACAGAAACAGAAACCGAAACAGAAATACTGACACCGCAACAGGACTGGACTGTCTGTACAAATAAGCCAAAATAGCTTAAAACGCACAAGTATAATTACAATTTAGGCAACACTTAGCACAGTACACGAAAACGTTATCCAAGGGCCCGAATTCCGTGCCTCCTGCTGTGGCAAGGGTGAACCAAAAAGGGGAAAAAAAACAACAACAACCCAAGGCAGAATAAATAGGGGAACAGGGAATATGAAAAGGAGCTGGCAGGAGCAGAAGCTGCCTGCCAGACCCTTCAAAGGCTCGAAACGACAGGCCCAGAAGACAGGCCGCCAGCGGCGAGAGACGATGGCGTGAAGTGAAACTGCCAGTCGCGACAGCTGAGCCCCAGATCCAGATCCCAGTTCCCAGTTCCCGGTTGCCAGTTGCCAGATCCCCAGACCCAGATCTCAAACCCGGGCTCAGTCCCAGTTCCGTGTGCCACGAGCTCAACTTGAATTATGTGCGCCCCGTCTGCCAACTCGCAAACTCGCACTCGCAAAAATAGTTGTTCAAACTTAGAACAATTATTACAATTACACCCATAACATAATTTTATACATTGTAATACCACATTGGTAGCTTGAATATTAAGTAAAATTTTAAGAATGTGATATACGCAGCATTCTCTCAGTGTGCTCGTGCTGCGCGATGAATGGGTCGAAGGAAGTGGCGTTGAGTACTAGCATAAGGAAGCATAAGCTGAGCCAACCGAGCGGCACCCGAGCCGCAGAGCCAGGCAGTTTATGCAAAAGCAGGGTACAACCAGTCCAGGGGAAGCTGCTTTGGATCT

CCTGTTGCCCCTAACTCCTGCCAATCTTCCCAGCCGCGTCCCTTTGTGCGCTTTTGCTAACATTGTGGAAGGAAACGCTGCTCGCGCTAATGATGACGATGATGTACTGCAGGACGTGTTGGTTTTTGCTCTTTGCTCAGCTCAGCTCAGTTCGTTCCTGTTCGGTTCGGTTCAAAACAAATTAAAATCAACCAGGAAGACGGGCCAGGAATTGCAGTGCAGTGCTAAGGACAGGATGCCTCCGCCGGCAGACTGCTTTACATGATAATTTTATGTTTTTACGTAATTATGCTAATGGATGGATTTGCCTGGCGCCCGAAACCTTGCGACTTTGTAAAACTGCAAATTTATATCAAAGTGTTAATTATGCTAGTGGAGCGGAGCTTGGGCCAGCAAAGCCGAGCCGTACCGAGTCAAGCCAAGCCATTGGCCATTTAATTTACTGCCAGAAAAAGATGCCTTCTATATATCCGTCTATATCTTCGCATCCACATTCTTGGCTACCTGGCTCCCATGTGAGCGCATAAATTTTGCGCCGCATGTCAGTGAGGAGCAAAAAAAAAAAAAACAAATGCCTTTATGATTTGTCGCAAATTACTTTAGAAAAGTTCGGAACACCGGGGCTCACGTTCGACAAACAACAATAAATCACGGCAGTGGGAGCAAATGTTTGAAAGCCATTCTGCTGCACATATGAAAAAGTTTTTGCAGTTGACGCCACCTGTAACAGAATTTAATTAACAAATTGGATCTGCGTATGGCAAATGTACATCAAATGCTTCATTAGGCACTGAAACATCTTGATTTCGTATGCTGCTCATTTTTTGGGTCGATTTGCTGGCAAAGTGGTTACAATATGTGTCATTGATTAATTGGCAGAACCTCATCGAATCACATTTAATCTCCCCACGTTCTGTAGGCCACTAATCTGTGCGGTGA

 

---

Search Again