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CRIMIC CR01118

Gene Eip93F

CG18389

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	3R:21981949..21981969	93F14	FBti0202678	81178

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
short band	OK	TGTTTGATAGTCCGGCAACGACGACT	GGCTGCCAGTTGCGTTGTCTAATTGA

Images

Construct Design Sequences

sgRNA sequence	TGTGTAACCTTCGACCCGCTTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
ACGACAATTCCCTTGACCCC	TTACACAGTGTCTGTCCGCC	CAAGAAGCCACTGGAAAGCC	AACCCCAAGCCAGTGTCAAA

 

Amplicon 5'

Amplicon 3'

ACGACAATTCCCTTGACCCCTGACCCCCCGTGGACAATTGTGGAATTTTTCCGGGGCTAAGTCAAAGATAAACCCCGCGCGAGGCATCTCAATTTAACCTAATTGCTGTCCCTTATCCTTGCGACCAATAACATGGTATTTCAATTGTCCGGCATAAAGAGAATTCCTTTGCCATACGGGGCGTATGAGTAATGTCTGAGAATATTTGTCACGCTGCGGTCAAGCAAATAGAAAAACAACCTGTTCGTCCAAGCGCACACACACTCCAAATTTGACACATTTGCTCAGTGCAGGCCCGAAAAGCCTTAAAAACTAACTGAAAATGCATTCGCGCATGGAAAATGGAAAATGTGCTAAGCAAACGCAAATGAATTTTGCAAATTCGAAATTCAAACGTTAAGCGACAACATAGCAGAATGCATAAATTGAAAACTCATGTGGAAACGAATCGAAGAAAAGCGGGTGCAAAAAAAAATGATCGAAAGAATTCTCTTTTGGAGAACTGTCATCAAGAATTTGCTTTTTGTATGCAAAACAAGTTCTGACTTTTAGATTTTGTTCGAAACCTTGGTTTGCTCAGAAAATGCGCTTAAAATTTGCTGAAATATATAAAACGCGCAGTTAGCCTTTATCGGAAAATAAACAGATTCAATTTTGCGTCAATTAACATTACAAATTGGGAATATAATTCTTTTTACATAAAAAGAATGAAATAAATAGCAAGAATCGAAAATAAATGTTATTAGAAAACGTAATATTTTGCTGTTTTTTTTTGACATTTTGCTTAATGATGCTGCATTAAATGACAATCAGCCAAGGCGAGGCTTCAACCTCCAACACTTTTTCGCTTTTCCCGTCCAAAAGATGGAAAGCCTGGCCAGACGCAAACAGTTTACTCAGACGGATCAGGCGGACAGACACTGTGTAA

CAAGAAGCCACTGGAAAGCCGGGCGAGGAATATGTATACAAAAGTAAATATGGGAAATTGTTTCCATTTTTTGTTGCTGTATTCCTATCATTGTTTGCCAAAGTCTGGCAGGTCGGTCACTCAATACCAATTGAAAAGTGCTGCAAAAAAGTGCAAAAATAATGTCTCAATTTCATTTAAATCGATTTATATACATCCAAGCTAACAATTTAGGCATGCAACAGTTAGGAAAATTTGGTTTTTAAAAGCAATAGAATAGTATGTTTAAAACTTAGAAATCGCTAAATGGCAAATGAAAAATTATATAAAAAGCGGCAAACTGAGAGATCTTGAAATGCGGGAGTGGCAAAAAGCACGACAGCACTCACGTGTATCTGTATCTAAGCGCCAACGAAGGCAGCGATCAGATACGATCCATTGTTGCCACCGCTCACACACTCGAAAACCATGAACTGGAAAACCTTGAAAAACTCAACTGCAATTTAGTTTCAATGCAATGTGTTTTTATTTTTCTTATTTTTTATGCATTTTTTTCCCCCTCTTCGCCAATTTGTACCATCTGATTTTCTTGCTGCGCTCGATTTGTTTCTGCGCGATTTCCTAATGAATTCGATTTGCGTGGAAAACCTGACAAACCTAAGGCCCTAATGGCCCCACTGAATTTCATTAGCCATCCAATCAACGCAATCTGTTTATGCGTGGCTCTTCAGTTCGTTTTGGTCTGCGCGTGTGTTCTTTGGGCTTTTCTTTTCCTTATTATTTTTCATTTCGCATTTTCTTTTTTTTTTTTTTTGTTGTATCATGCTCCTTTTGCACTGTGCACTCAGTTGTAATTGTGTTAGAGCAGAGGCCTATGCCACTGCCACGCATGCCGCAAATGCATTATTATTATAATTTCCAGCTCATTCCTCGTTGTATACTTTTTTCTGGTTCTTTGACACTGGCTTGGGGTT

 

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