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CRIMIC CR01120

Gene eys

CG33955

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	2L:2326415..2326425	22E1	FBti0202679	81179

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
short band	OK	CCATTGCAAATATCGCCACAGTCTGC	AAATAAACCGCATTCAGGCAGCACGG

Images

Construct Design Sequences

sgRNA sequence	AACAACAGGCGAGTGTATCTGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TAGCTCCATGGGAAAGCTGC	TACACTCGCCTGTTGTTCCC	TTATGCCGTGCCAAGACCAT	CACTGGATCCCTGTGTAGCC

 

Amplicon 5'

Amplicon 3'

TAGCTCCATGGGAAAGCTGCAAGTCAAATGCGAAATGCGAAAACCAGAAGCTTGAAATCGCTTCCCGCCTGCATTTCCCCTCCCCATCAACCAATAGGAGTGGAAAAACTCAGGCTGGCCAAGGCGGATGGATGGGAAAGTTTATATGCTGGCAGGCGAAATTTATAGCTGCAATTAAATTTAACACGATGCCATGAGATTTAAACGGATTAACTGAATAGTTTTCGACCAGCTCAAACTTTGGGGTCGACTGCGTCGAGGGGGAAATATCGCGAGGAGTTTACTGGCTTCGTAGAACATTGATATTTTATTGGTTTATCCTGGCTTATTCGAGCGATAAGATGGCTCTTAACCGTTTTATGACTTCGCTCTGCACGCTCCAATAAAAGTTGGCTATTCAAGATGAGCAAAAGGATATGATTTGCTTGGCAAATTGCCAAAGCCATTAATCGTGCACTTCATTTAGTCATGATAAAATCGGAATGAGAAAATTTCTAAATTATGTCTTTAAATGCTTTCGATTAGAACTCGGCGCAGAGTAATGGTTAAATTATTATGCAATACATTATTGATTACCCCCAATATTTATAAATAGAGTAAATGCTTCGCAGGACCACAAAAAATTAGCCACTCCAGCGAGCGTCCAATATCCTGATGGCCAGGCACTCTGATATTCCGTCCACAAGGAGCCAGTCCAATGCCATTACGTAGTCATTTCCCAATTGCTCCTGGCTGAGCACAACAAATCAAATAGTCTCCCGGGTGAAAATGTCCAACCGTAGCCCATAATCAGCCGAACTGGAATCTCAGGGGCTTCACTGGTGAACTGAAAACAAGGGCCGGGAACAACAGGCGAGTGTA

TTATGCCGTGCCAAGACCATAACAGACATGTGCACCGGGAGCCATCAGCTGCCTCCTGCATCCTAATGTAATCCATTATAAGGGACAACAGTAAGCCAAAGCTACCTACATACCCCCGCACCCCAGCCCCACTGGCCCATTTACACGGCAAAGAAACTTCTTTAATTAAGCCGGTTACCTGGCAGCAGTTCCTGTTTGCCATGTTTGCAGTGCTCCTCGGATAGCACTCGTCCACAGTTCCCCTGACACTTTTTTCGCTCTTTGCATTTCCGTTTCCGTTTCGATCCGAACTCGGCTTGGCCAAAATATAAATAGAAATGGAAATAGAAATAGGAATGAAAATGGAAAACCCAATCCCAAATGTCTACCAAATATTTATTTGCACAACTCCTCAAGTCCAATGTTGCGCTCTTTGAATGTTTATAGCACTCAACTGGCCGGCGATTTAACTGCAGGGCATGTGCATTATGTCTGAATGTAGGGGATCTGAGCCTGAAAAGTCGGGGAAATTTATTCAGGAAAATACTGGACTGCAGGCCAAAACAAATGCGAGACTCGAGTGCCACCAGCTCAGTTCCATTTTCATAATTGTGAAATATGCATATGCAGAATATTAAGATAGAAGCAAAAGTAGTATAAAATATAGATGTATGATTGACTAATATTGTTTAAAGAATTGATCTAGATATATATCTCAGGGAGATGGCGAATTTATACGTGTCCGCCCATCTGCACTTCAATTACTGCCTACTGCCATTATAGTCCGGCTGTATCTGTATCTGTGTATCTGTATCTGTTCCTGCCCTCCCGAAACAATTTGTATCCAAAGCATTTGGTTTGTTCTGCCACTAAACCTTCGTTTTCTCTGTTTTCTTTTGGTGGTGCTGCAATCCGGCTGTTTTTTACCACCGTTTGATGCATCATTGACACACCGTTCCGCACACCCACCGCACCCGGATGGAAACAACTAAAACAACAGCTCGTACTCGTGTTACTGCATTGATGGCTACACAGGGATCCAGTG

 

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