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CRIMIC CR01124

Gene Mmp2

CG1794

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	2R:9615301..9615320	45F7	FBti0202680	81180

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GCCAGACAATCTACATGTCGTGGCTT	AATTACAAAACGCAACCGGCAGGCTG

Images

Construct Design Sequences

sgRNA sequence	GGATGCATGGATACGAGCACTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GATTTTGCCCGCATGTGGTT	CACTGGTGGTAGGTGGTGG	CAATCGCCCGATTCGCAATC	GCACCTAGAGAATCCTGGCC

 

Amplicon 5'

Amplicon 3'

GATTTTGCCCGCATGTGGTTTCGGTGTAACACGCCCTGCACTTGGCGAAAAACATATGTCGATTATGACAACAAATGCATTGTACAACCTAATTAGTCAGCATCCCCTGCACATCGTGCTTTACTGCTATTTAATAATACTAACAATAAGTTTTCGAATTAATAAAAATAAATGGCAATTTCAAATGATATTCATAAAAGTACGTATCACGCCCTGCACTTAACGAAAATGTGGAAGTCATTCGTGACAAAAAATCTTCATTATTTTGCCATACACACTACAATTATTTAAATGAATAATCAAATAGATAGCAATAAGTTGCGTTTGTCAAACAAATTACTATTGAAAATGTGTAATAAACGCCCCACACTTAGCAAACATATAGCGGTCAATTTTGCGTTATTACACAACTTCATTAATAAACTAACGATTTAAATATAATCGAATTTTTCCCAGTGCAAACCCAAAAAAACCGCAGACCAGACAGCGCCGCCAAAACACTATTGCCCTCTAATGCAATTAGTTAACGTCAAAAGAAGAGCATTATTATGTGGCCTGGCCAACGGACTTGATGAACTGCACCCACCATGGCTATCATCGGATTCGCCGTCAAAGTTCCACGTCTCGTCCGCATCGAAATGGGCATCTCCGCCACGCCCCTCGCCGGGATAGAAGGCGTGGGCCAGCACCTGACCAGGTCCATCGAATTTATAGCCATCTCCGTGCGCGCGCCTGCAAAGTGAAGAGAAAAGGACCAGCGTAGATGTCCTGGATTAGTTCGGTGTTCCAGTGGAATTACGAGCATGGCCAAGGGGGAAATCATATTCACAGCTTATCTAAGAACAAGCAGCTGCCACATAAACTCTTTGATTCAGACACGGCAGCCATGGCCATCGACTTTGTCATCGGTGGCATTTCATCATCGACGTCGTTGGATGGCCATGGATTTAATACTTGGCTTATTTATCGTTCGCCTGCTTTTAAGTGCAATTTTATGTGCGTTTGTTTATGGCACTAAAGACTCGCTTTTTTATGATCTTCCCCACCACCTACCACCAGTG

CAATCGCCCGATTCGCAATCCCCATAGATTTCGGAGTACTTACCGCGCAAAGAGTATCTGAATGTCGGCCTGGTCGCTGTAGACCTCGCGGAATGTCAGCTTCGAGTGGTTCGCCCAAACGTCCAGGGCGGTCTGCACCATTCTCTCGACTTTGGAGGCATCGGGCATCGACCTGTTGACCATGCTGCAAGGAAAGACGAAGGAAGAAACATGATATCAGCATGCTAAGATATTCCACTTTGTAAGGGTTTCATCCTTTGCATAATACACACAAAGAGAATAAAGTGATATGGTATATATATCATGGATATTTATATTTATATTTATAGTTATATATTTGTGGCAGCATTGAATGCTTGGCATAATTATACCTGATGGAAATTATAATAAGTAAACCAAAGTCCCAACTTAACCTTTACAAAACCTTCAAACAAGTTAGGATTGTCCTACTATGGATATAAACAAATTTTCTTTCCGTGCACGCCTTGTAGGCACCGAGTCTCCGTCTTATCTTGGAATATTTTGCAAACCGCTAATATGGTGGCCAGTGCCAACTTTCACCCGCCCACACACTCCACTTTCACTTTGTCATGCTAAAGTTTCGCTCACTTAAATACATGGGCGGCGGGTCGGAGTTTGGTGGCCTGCACTTAGTTTAGTATTTTGTGAACTCTGTCTAAAAGAGTCGGGCAAACACACATAGTACCTGAGAAGTACCAAAACCTGGCTCAAACCGCAGCGGGTTTGCCTTTTGGCGTCAACTCTCCACTGGCGACTTTGCCCCATTTCCGTCAACAAAGTTTCCGTATTTCCACTCCCGCCGCTCCGGACAAACTGAGATATCTGTGTGCTGCTGCCGTGTTTCCGCATTTTATGCAAATTAGTTATGTTTCTTTTATGCCGGACCCAGTCTGTGGCTGCTCCTGCTGCTGTTAACTAACGCAGAAAAAATAGGGGCCGAAAGTTGATCCTTTTTGCGGTTTACCCAGGACCTTGTTTGCCTCTCTGCCGTATGCTTTCTCCATACCAATTTGCCGGCGCTTTTTATTCCCTTGCCTAGTTTGACTTTCAGCCATTGTCTTTTGGCCAGGATTCTCTAGGTGC

 

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