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CRIMIC CR01126

Gene CG43366

CG43366

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	2R:5921811..5921839	42A3	FBti0202682	81182

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TTCAGGTGCTTGGCAATTTGCCTGCT	TATCCCTGTCGCGTTGCATACTTGAC

Images

Construct Design Sequences

sgRNA sequence	AGGTGGTAGCTAGGCAAAGGTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CACGGCATTCTTTACGCTCG	ACTACCTTAGAGCCAACGCC	CAATCGAGGGCCAGACAACA	GCACACACTGCGCTTTACAT

 

Amplicon 5'

Amplicon 3'

CACGGCATTCTTTACGCTCGCCTTCATTAACATTTCATTAATTAGTTTTTTATTCCACTTTGCAGCTTTTTCTTGATGTTTATGCATCGTCCTTCGACACCAAGCACCAATCGGATTCAGTGAGCCAAAGGTCCTGACCTCTCGAAAGCAGAAGGAAGTGGAAAGCCATTTTAAGATTAGAACGAGGCTGAAACACAAACAAACGCCCGGGACTTCACCACTCCACAATGCGTCTACCGCGCACTTTTCCAGCGCTTTGGCTCTGGGCCGTGCTACCCTTGGCCACCCCCGGCATTCTGGGCTATCCAAACACCCAGTCACAGCCGGCACTGGTCACCTATGTGACGACCTCGTCGTCGTCCAGTCAGCGGGGCCACTCCGCCAGTCCGCACCTACAGCCCGCATTCGTCTATGAGTCACCCCTTCCGGAAGAGGAAGAAGGTTCCAAGCATTCACTGGCCACATCCACATCCTCATCCACATCCGATGTCCGAGACGAGAAGACGACCGAGTCGTCAAAGAAAGTCATAGGTTCCAGGTGAGTACACACACGAGTTTTATACTGGGACTTACGCTCATCAGAATTAAGGAATCAACACTGAACTTCTTACCACTACGAGTATCACTGAAAATAACTTCAAATATCCGACTCGCCTAGTATAAATAATAAATATAGTATCGGAATTAGATTAGTTTTCCGAAAAATAAGTTAAAGAAACAAACTTAAATATTATCAGATTGAGCTAGATTATATTGTTTTTAATCTCCAATCTTATTATTTTCTGTGTACAAGGGTGCGCTATTTTTCCACTTCTAATTTTTTTGCCCTTTGTTTGTTGTTTCTATCGGTGTTGCTGTTGCTAAGGAAAATCTTTTGTTTGCACAGCTTTTTGCAATTTTTGCCACTAATTGAAATAGAATTTCTGCTTTGTTGTAGCTGCCGTTCCGATTGAAAAGCGTGGGTTGGTCAGGCTGGGTCCTCGCCTTAGTATTTTTAATTAATTTGCTCTCAGGCCGCAAAGCGTGCTGGAGCGGGCAGAAAAGCATAGCATACTTTGTGGCACGCGTTTGGAATCCCATTGAGTTGGCGTTGGCTCTAAGGTAGT

CAATCGAGGGCCAGACAACACTGTCCAAATGAAGTGCTCATTAACTTTAAATCAATGCTAATCAATTGGGCTTATGTGGGCGCGATGTTATTGAAGTGGGCCCCAAAACGGCTTAACATTAAGCAGAGAGAGATAGAGATGGAATACAGCAAAACAGAATTTATTGGCACACTTTTCCTTTTCTGTGGCAGGCCCGTCGTTTTCTCAACGGGCCTTAGATCGTTCATATTAACGGGTTACGGTTTTTTGATTAATTCCACTGTTCGCAACCAGCGCCCTTCTGCGTCAGCTGTTCGCCCTCCTTTTTTCCTCCTTTTACCGTTGGTAATGACTGCGAGCTGTTGCCAGTGATGGTGATAACGATGATAATGAAATTGATGACTCGCAGCTCCGTGTTGAAAACTAGCAAAACAAGTCAAGCGCAACTGCAATACTTGTATTTACATGTATTTTTGATTGTGCTGCAATGCCCTCTGGATTCCAATTAAAACCAATCCGTGCGCTTGACATTCTTTGGGTCTTGTGCCACAAAACGTCACTTGGACGGCAATGCTTACCTAAAAATTAAATTGGAAATAAGTCAATAAATCATTTTATTTGCCCCAAAGGCCAGATCGGACTAAGCTGCAAATCGTTGGGATGCAGTGGAAAATTCACTCAAATGCTATCGAATATTTTCGTCCTAATCCCATTGCAACACATTTCTGCACTTAATATTCGCATGAACGGATTTGCCTCCACCGCGCTGATGATTTTGACGAGGCATCATACCCGGCTTATGGCATATGGCATATGCTTTTATGTGTGTCCATACATACATATATAGTATGTGTGTTGCAGCTGCATATGACTAAGCACGCTTCGACTCACGTGCTTAAATGTTAATGATGTGCTCATGCCACAAGCGGAATTACTTGCATACTCATATCATACAGACGCACTCACAGATGCAACATAACATATGTAAAGCGCAGTGTGTGC

 

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