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CRIMIC CR01129

Gene Sb

CG4316

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	3R:16139950..16139962	89B5	FBti0212923	91251

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
no band	OK	TTTCTGGCTCAAAGGAGTCTGCAGAC	TCTTACCTGATCATCGGCTTGTAGGG

Images

Construct Design Sequences

sgRNA sequence	TATAAGGGGGCACGTGCAACTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GGCGATAAACGGGATTTGGC	TGCACGTGCCCCCTTATATAC	AGCGATGTGTGCTACCAGTT	AGCCGCTACTCATGTCATCG

 

Amplicon 5'

Amplicon 3'

GGCGATAAACGGGATTTGGCTGGAGCCGAGCTTTGAGTATCTCTTGTATGAGTGTGTCGAATGAATGATGTTTGGATATCTACGTTTTCCAATGAGTGTTCGATAAATATGAATTAATCCATGAGAGAGGTTTGCTCCGTACAAATTCGTATAAATATAAATTCATTTACATATGTATTATTTGGTTTTTAATAGTTTTTCTTAACCTGTATTTACTTACCTTTGTCATCGGAACATTTTAATCATAATTGATTAATTTATTTAGCCCATTTAAATAAAGTTCTAGCAATTACTGACTAATTACTCACTCCAACATTCATTCGCAAGTTCCTTCACCTCAGCCATCCATGAGTTGCATAATATTCCCAAGTGTCACATAATTGCCACAATCCCGCCCCCAAAGTGTTGTCAGCACATCCATTCACATGGCTCCTTTCCGCACTCCTCCAGTTATCATCGTTGCATATGACCATCATCATGATGATGGCGATCTTGTGATTACGATCTGTGGCCGGCTAATGCCGCAATCTCGTGTGTGTGGGTTGTCTGGTGGGTGGGCCGTTTGAAGCACCACCAGATCGACCGGCACCACCACATGCATGCCAAACTAAAGTTAGACATCCCGCAGTTAGCCTACTAATTACAGCCGGAGCTCTTTGCGCCCGGCATTCTCGATGCCATTACAAACATAAACCATAGCTTGGCGCAATAAAAAAAGAGCATACGGTTTTCTAACAACAAACTTTGGCTAACCATACTGATTATTACCATTCTCCGTGCAATCCATTGCAGACATGCGCCCGGCACGTTACCGATGAAGCAGCCAACTTTAATCAGACCCAGACTCAGACACAGGTGAGTTGACAACTCGAGGGGTGGTGGGGATGGTTGGATGGGGTATATAAGGGGGCACGTGCA

AGCGATGTGTGCTACCAGTTTAGGTTACAAACCATAAAGGCAAACATCTAATTTGGCCAGCAATGGATTGGCTAAACTTTAATTAAATTTGTTGAAAAGAAAAAAGAGCTATTCCGCTTTGATGAGCTGCAGCGAAGAGGAGTACGAAAGGGGTGGGGCAGCACTGGGGTGCACTTGGTTTTGGGACAAGAAATTATTAATTGTAGCATGTGTCGTTTGACGTCGCCGTCGTGCGATCGCAATAAATTTTTTCCAGTCTTGTTCAACAATAAAACAAGACAAGAGCCACGAAAAAAGCTGATTATGAAGCAAAAGTGAAAAGTTGTAATTAAACCAGTCACTGACTGCACGGCGAGCAGCACAAAAACAAACTGCAGCAACATATGCAGCAGTAGCAGCAGCAACTGCAGCAGCAGCAGCAACAATCATAAAGAGTAGAAATCGTTGCCTGACTTTGGTTTTTTTTTTTGTGCTGCCACTTTTTGTGGCGACAAGTGCGCTTAATTAGTGGTGGACTCAGCGGCGGGGGGTGGTCCCAAACCCAAACAGGGTTCTGGTGGATGGGTTCCGGCAGGCGAAACAAGACTAATGTCCGAAAACTGTCATGCCCGTTCCTTGCAGAAGAAGCACACCAGCGGCAGCCACCAAGATGTGTCCCAAAAGGCATTGGCTAGTCAACAACAGAGCAGCGGGATCGCGAGGATCAGGAGGAGCTGCGGCGCGGAGCAGGCGCAGTCTAGACCAAATAGTAGAAGTATTAGTAGCCTTAATCGTAGTCAATTGCCTGGCCACAGCAGCAGCGGCGCTGATCACACCGCCCGACAGCCTCGAGTCACTGGGCTCCCTGGGAATCCCGTCATCTTCCGCCTCCTCCTCCGAGGACGACGATGACATGAGTAGCGGCT

 

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