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CRIMIC CR01132

Gene Men-b

CG5889

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	3R:27153333..27153352	97E5	FBti0202683	81183

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
no band	OK	CGCCAAGGCGTCCGTGTATATATACA	TAGAGATACTTGTTCAGCGGCTCCGT

Images

Construct Design Sequences

sgRNA sequence	GATGGTGCTAATTACGAGAGAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GATTGGGAATCGCACATGGC	GAGAGGCAAATATCGCGGGA	ATTCGAACTGCCAGCTAGCA	ACGGATGTGATCTATGCCGC

 

Amplicon 5'

Amplicon 3'

GATTGGGAATCGCACATGGCCTGGCATTGACCCTTCGGCTTCCCTCCCTTTCTCGCACTTGCTGGTCCCGTTCGGTGAACAGCTGTTTTCAGAATTTTCACTTTTCCCTTGCAATTGCATAAGCGTTTTCGAACATGCATGTCTTCGTTTTTTGGCTGCCAAACAAGAAACAGCTGAAAAAATCAATGGGCTGCAGAGAGAGAAACGGGGTGCTACGTATCATAAGTATATGTGCTTTAATTTTGCTTTTCTTCGGTGGAGGAATATCGCCGCACATAATTCGTATGAATTTTCTATGATTCACAGTTGGATAGGAAGTACAAATACGAATGTATTCCATTGTTTAGTAGTATTCTAAGAAAAAAAAAGGAATGGAAAGTTTATCATCATACTTTTACAATCGAAAACATTTTGTTTCCTGTGTAGCGAAAACACAACACCTTCAATACCATGGACATGGACATATATTCGAAAGATGGTCTGGCGGAATTCTAAATTTAGTGCATATACCGAAAAAATGTGTGCTTTGTTTCGCCGCTGTCAGCGTTTTTCGTTGCCAAGTTCATCAAGAATGTTTTCAGCTGGTCTGCTATCTTGCCTCTCGTTCCCACGCAAACCGTTAGCCAGAAATTTGGCTAACAAAAAATGGAAAGACGTAAAGAGTTTAATGAACTTGCCCAGCTTTTTCCTTCAGACTCCAGTTCTTAGGGCTTTTAAGACCCTAAAAATAATTATTGGTAGCACTCGGAGTATGGGGGTATCATTTACGACACGCACTTATTCCGTGTATTTGGCTCAGATATGCGAAATTGTTTAGCACTCGACGTGGTGGGGGCTCATGTGAGTGCCATTCAAATAAATGGAAAAAATTGACGAGTGATAGCGTTACCTAAACGATAATTATCAACCCAAAGTGCTCGCCTTTTGTCGGTGCAAGGGATCTCGGGGTTTCCAGCCCGGCGCACGTCACTAACCGGTTTCAACTTGACCAAAATCCAGCAACGACACCGATACCGACCCTCTGCCAATCCCAATTCCACTCCCCGCGAGCATGATTCCATCAATGAACCGCAAACACAAATTAGTCACACGCCTCTGTCCCGCGATATTTGCCTCTC

ATTCGAACTGCCAGCTAGCAAAAACCAAACAGCAGGGGTCAAAATTATAGGGTATCTTATAATATATGATCTTCGCTTGGCACTTCGATTACAGTAAGAGTGTTGCATAATCAGAACCTGTTTTTAATTAACTGATCTAGTCCGTTGGGGTCCATAATATTGTATATTTTTGTAAGCCCTTCACTACGAGTTGTTAGTTTTGCAATCTTATCTGGGGGTATTTACGCTTCAAGAACAGAAAAACATTTTCAAGTTCAATGATCCAATTGGTTTTGTAATTCATTAAATTGATACGATCACAAGTAAGTGCACAGAAAATTACCTACTTTAGTTGTCCCTAGAGAATAAATAATAATGCACTTCAATCCAAGCTGTAGAACTGAACTTGTAGTTTTTAAATATTTTTTATTCTAAAGAATTTAGATAAGTTATTTATAAGAATTTATTACAAGTTATTAAGATTCTGAGGATTAGATGCTAGATTTATGACCGCGGCTGTGCATAGATTTTTATCGTGATGTTGCCAGGCCATCCATCCCCCTTATCGCTGGCGCTTTGGATTCCGGGGGACAGCTGTAAACAATGCGGCTTAGTTTCCTTCTTTTCTGCTTTTCATTTTATATTTTGGCGTGCCCAGCGCGGTGATAAACAAAAAGCAGCTGATGTCGACCTTGGGCATGGCGTTTTTATTCCATAAATATATGCATATGTGTGTGTTTGTGGGTGGACAGAGGGGGCCCCCCCATTGCCAGTGGCAATGTCAAGAATGACAGTGGGCCCACCTCAAAGTCAAGGGTTCCCAGGGGGTTCTCCAATTGCCGCGCTGATAAAGTGATTCACGTAATGCACTTTTGCAATTTGCAGCTTATGTGGAACTGTGGGCAAATTGTGTCGATGCGGCACTTCAGCAACAGGAAAGACTGCTGTGGCAGCAGCAACAGTTCCCACCGCTCGACACTATCATGAGGTGGTGGGCGATATCATCTGCCCGTCTCAGGTGCGCGGCATAGATCACATCCGT

 

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