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CRIMIC CR01134

Gene Men

CG10120

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	3R:12715404..12715421	87C7	FBti0202685	81185

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GCATGATGTGGCAAGTTTGGAGGCAA	TCGACCTACCTATCCACCATCCAGAT

Images

Construct Design Sequences

sgRNA sequence	TGCCCGACTCGTGAAGTCGAGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CGTACAGATCTCCAGTGGCC	ATCTCCTCGGATCTGCGAAG	CGAGTCGGGCATCAAGTTCA	GTATTTCACCAGCCCACCGA

 

Amplicon 5'

Amplicon 3'

CGTACAGATCTCCAGTGGCCCTGCGTTCGCGCAGACCGATGTACAGGGGATCCTCCAGGATGGATTCGGTATTGGTGCCCACATCCAAGGTGATGGGCAGGCACTGCGATGGCTTAATGCCCGCCAAGGCCGTATACAGGGACAGTTTGCCCACGGGTATACCCATTCCGTTGGCGCCCAGATCTCCCAGTCCCAGGATGCGCTCGCCGTCCGTGACAACGATGGCACGCACATCCGTTTCCGGCCAGTTCTTTAGCACGTCGTAGATGTGTCCCTTGTCCTTGATGGATATGAACATGCCCTTGGCGTTCTGGTGGATCAAACTGTAGCGCTGGCAGGCCAATCCCACGGTGGGCGTGTACACCAGTGGCATCATGTAGGCGATGTCTGAGCTGAGCACGTTGTAGAACAGACGCTCGTTCCGCTCCGATAGGCTGATCAGGTACATGTACTTGTCCAGATCCTGATCCAGTCGCGCCAGCAGAGCGCGGCAGTGCTCCACCTGCTCACTGGGCTCACGGACCACATAGGGCAGCATGCCATGGATGCCCAACTGCTGGCGCTCCTCGTGTGTGAAGGCCAGGCCCTGAAATTGAAAGGGATATGCATTTTAGTTACCAATACCTACTATTAATATCTGTAGGATTCGTAATAGGATGGCATGATCGCCTCATTGAATGATTATAGAATCTCCTCTCAAATCAAAACAAACAAAATGTAGGATGGTGGAAAAGTTTGACCCGCTTATCATGGACTTGAATTCCCAGGCACGCGCCCCTATTCATATAGAGTATTCCACGGCTACTAGCCACCGAGACAGAGGCAGTTATTACCGATGCTTCGCAGATCCGAGGAGAT

CGAGTCGGGCATCAAGTTCAAAGTCGTTTTCTAGCGCCCAGTCGCTTGAAAACATATTTGCAATCGCAGCTTTAATTAAAACACATTTTTTTTCGAGGGGGAAGTGAATCAGCGCTGGCCTCGATTCACACGCACAGCTAATATGTTTAGTACGAGATTTTGAAAGTTTTTGCTCCGTAATTGCTGCGGTTATTATCGAAATGGACAAAGCAGGCGGGGATTATGTTGGGGGCGTTGCTGCCGACATTAAATAAAAAAATACGTGCAAGTTGGCATTCAAATAGAAATCACGCTGGATTATGTGGTTTTTGTTTTTAAACTACGGATGTACCTGCTAATATGCACATTAGGGAATAATTACTCGTGCTTTGGGTTACTTTTCCGAGTGGCTGCTGTTTTCAAATCGTGCTCATTACAAAGGTTTTCTTAATGAGTCTATTTTAAGCTTCTTGGGACATTTGATTTAGCTTAATAATAAATGCACATTTTTATTTCCAAGCGATATATCAAAATGCAACCAATTCCAAATCTGTTGTGTAAGTTTTCCGAATTATTTTTGTAAATTGAATATTTTGTTAGTATGTCAAATTAAGTTATTATTGGAAATTATACTTGATAGGTTTTCTATCATTTATCATCATTTAAAATTAATCTCGTTTGTTTCGGTCACGCAATAATAAATTTATAGTTTTTCATATGAATTTTTAGTTTTCCGATAAAAACAAACTATTCGTATTCTAGTTAATTGATTCGTAATTCGTATTCTAGTTAACGTTTTTGATGCCTATCACTATGAATAAGCAATATTTAAAATAATTTTGGTCAGCTTGCAAAATATCATATATCATATTAAGAGCTGACACAGATAAATAATATTGTTGTGTTTTTAAGGAATTTTAGCTGGAAATCGAGCTGATTCCCCTTTTTTTTTTTAAGTTTGCGAAGTAAAATACATATGTATTTACATACGCATCAAATTCAATTAGCTCTGAACTGGCTGTTTGGACAATAAACACTGAAGTGATCCCCATAAACAATTGTTTACTGCCAGAATTGCCTCGAACTTAACCATTATCGTGTGGCTACGTCATCGGTGGGCTGGTGAAATAC

 

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