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CRIMIC CR01141

Gene Sema1a

CG18405

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2L:8577702..8577722	29E2	FBti0202687	81187

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AGCTTCAGCTTGATGGGGTCACTTGA	AAATAGATCTCCCACTCACGGGCATC

Images

Construct Design Sequences

sgRNA sequence	ACCATCATGCCGCAACGAAAGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CCAGTCCCTCAGTCACACAC	TCGTTGCGGCATGATGGT	GAGGGAGAGAGGAAGAGGCA	ACACGGCCACATCAGTCATT

 

Amplicon 5'

Amplicon 3'

CCAGTCCCTCAGTCACACACTCACTCACCCACTCATTCACTCATTCATTCATTCATTCATTTCATGCGCTCATGCAGACATTCAGTCAAGCGTACGAAATTCTTGTTTGTTTGTTTCTTTATTCATTGGACAAGCGGGGCAACTTCCTTGCCAGAGAACTTGGACACGGGCTAGCCGAGCTAGCAAACTAGCAAACAATAAGTGAGGAGCGGTCAGGCCGGGATTGGTAATCGGGCATCTGGTTGCCCGGAGGTCGGAATGGGGCAGGATTTCGAATGGCTCGACGTGGCCACTTGGCCACGTCACTCGACAATCAATCCTCATCGAGACGGCGTCGGAAATTGTTTTCACTCGCATTTGCATTTCAATATTCAACGATTGCAGCTGCAGGTTTCGACTAGCTTAAGTGTACAGAAACTGAATATATACAATGGGATATTTAATATTTTATTTTGGCTAACCATAATTTCTAACTGATGCTAAAACAGTGCAATCAAACCCCATTTAAGATATATTATAGGTTTAAGAGAATGTAGTCAGTAATGCAAATTTCACGATGTTTTCTGTAAACGCAGCTTTTCCATCCGGCATTTATGTGAACCTAAAACTAAACCACATAGAGAGTAACTACCCTGCTGAAACCACGCTTTTGGCCATGTATATTTAGGCCTTTATGTGAGGGCGGCTGAATAATGGCGGCTTGTATGCTTAGCGGCAATTAATCAGCGGAACTGTCCGCACCCAAACATGCGTGAATACCCACCGCCTACCATAAAACCCTTGGCCGTGTCAGAAAGAGATGGATGAATATGACAGTGAGGGGGGAGGAGTGTGGGGAATGCCACCCTGAAAATGTTTTGCTGTAATTAACTTAGATAAACAAAGACGCCAAAGGGACATTCGCCAGCAAGTGCGACTATGTCCTTCGAGGACCACCAGCACCATCATGCCGCAACGA

GAGGGAGAGAGGAAGAGGCAAGAAAAGTTGAAGAAGCAGTGTGGCATGGCCTAGAAGTCGACAGACTTCCATTCTCGTCTATATCTATATACATTTGTCGGCAGTTGAGAAAAATGATTGCTCAAATAAATCATAATTAGCCATGTAAACATTTAGCAGATGCAAGTGTGTGTTTGCGTTGAAGACAAAGCGTTGCCATAAATCGTACATTGTGTCCAGTCTGTGGGAATAAAAACTAGCTTCTATGCTACTTACTTTCTTCAATTGCCTGACATCGGCAGCCATGCAACTTGTACAAATTTATTGCCGCTAAAAATTAGTACGCAAGTCTGCATTTGAATTGTGTTCGATAAATCACACGATAAGCATATCATTTACATTGTTCACTAACATAAATCTGTAACAGATTTTAGCTTAATTTAATCTAAACTAATAAATATATCTAAGGAGAGATTTTAGTGAGGACAAAGTTTGTTTCAGATATGTATGTACATATATTTACCTGTTTAGGCGTCTACTCAAATCGATATTTTTGGCTTAGTCATGATATCAAGAATAAAGGCCCACTTATCCGCTGGCCATTGGGTCATCTGGTCATCATCTGAAGTTTATCGCCCCGCACATTGACCGCTCGTAAATTTCATAGAATTAATTACTGCATCTGTTGGCTGGCATTGTTCTTGTTGTCATTATTATTATTGCTATTATTGTTATTATTGTTTAACAGGCATTTGTTTGTGTTTGTGTTGGTGGGGCCCTTTTAATTTCTGCGCCCAACTTAATGATCCATGAATTACTGGATCGTGGGCGGGTCCAATTCCAATTCGAATTCCCAAGGGTCGAGGTCACAATTGCTATATGCTGCATGTCATTCCCGTTCTGCGGTTCAATGACTGATGTGGCCGTGT

 

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