• About
• Search
• MiMIC
  • Technique
  • Constructs
  • RMCE Lines
• Transposons
  • GDP
  • Others
• CRIMIC
  • Technique
  • Constructs
• Order
• Downloads

CRIMIC CR01142

Gene Nckx30C

CG18660

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2L:9741169..9741183	30C6	FBti0202688	81188

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	no band	GAGGATAATGCAGGCAATCAACGTGG	TTTCGGGGGTTTTCCAGCTTTTCTGC

Images

Construct Design Sequences

sgRNA sequence	CGGAGAGGGTACGATGTCAACGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
ACGTTGAGAGCCTGAAACGT	CCGGATGGGTATGGATGTTGA	GACATCGTACCCTCTCCGTTT	ATTTGTTTCGCCCACACACG

 

Amplicon 5'

Amplicon 3'

ACGTTGAGAGCCTGAAACGTCGTTAACAGCCACGTTTGCGTGTGCTGGCTCAAGCGCACACACACACACACACACGCACACACACAACCTCGCGCACTAGCTCACATACTTATACTATATTTCAACCAAGAGTGTGTGTATTTTTGTTGTTATTATACGGAGAGCGCGCTCGCAATTTGGGTCGATGGTAAAATAGAAAAGCCAAAAGCAAGGCAAATACACACACTCACACAACCATACACACTGTCAGGAATTTCTATATGTACCTACATATATATAAATATATATATAAAATTTCGAACAAAGGTCGCTGAAGAGAAGACCAGTGCACATTTATATAGAGGTAAGTACATACGTACATATAAATAAACGTACATATGTATATGTTTGTATGTATGTATGTATGTGCGCGGGTGACCTGAGTTATGTTCCCTCGTCTGTCTGCATTATTTTTCACTGTGTGTACGTCCTCCGCCGCTTTTTTCGTGGACTTTTTCCCGCTAGTTTCATGTTAATTGCTCGTGGCCCGTCGCTTGTTCCAGCAATCTGCGTTAATTTTAAAGCCTGATTTACACACAACAAACGCTGTACTGAATGTAGTTGCACTTGTACTTGCGCAATTGCGGATTGCAGTTTTTTTAATTCGCAGGACACGTCCCGCCTTCGAGTAGTTTTGTGTGTCACGAGTGCTCCGATTTGGTGGGGGTTTATTTGCTTGTGCGGATGGTCGGGAACTGGAGGAATGGAATTAAATGGAATGGGTGGCCCGAAAAGGCAAACAAAGCGCTGGATTGGATGGGATTTACCGCGTTGGCTTATCGAGGCTTGACATCATCAACATCCATACCCATCCGG

GACATCGTACCCTCTCCGTTTTATTTAAACAAAGGAGTCAACTCTAGTCGATCACATCCTTTTTGCCCCGAAACTTTTCCAGCAGCGGCAGCCACTTTGTGACAGGACTTGGCTCGGTTGAGTCATTAAAATGAGAAATGGCTCCGGGCTCCGTGTGCAATGTTGGGCTTAATTGTTGGCCAAAATGTGACTGCGCCGCTTACGGGAGCAGAAACACAGTCGCCGTGGACAGGCTGGCTGAATCAGGGGCATAAGGTAGAAAGGTTGCTGGGGCGCAAAAGGTTGCAAAGGTGCAGAATGTGGTGGCAAGAGCAGCAGCTTGACTTTCGCTAATGTGGCGCAGCAAGGTATATATATATCTATATATATATATTTATTCACATATGTGAATGTTTGCTGGGCGCACATTAACACGCTCTGTGGTGCATACAGAAAAAATATATATTTCATCGTCCAACTGCAGACAGTTGTTCCATTATGTTCATTTGCAGTCGCTTATGCTGCGGCCAGGATAATTTATGTTATGCCATATTCCGTATACGCAACGCATACGAGTTTTACGGAAATTACTGTGGTTCTTTCGCCTCAGTGGCGGGCACTTCAGGCATTGCGTTACGTATACGAAGTGTAAGCACATAAATATCACTCATACGCAGTGTGGTTGCAAAATAATTGCCATAGATCCGAACACCGTTAACATCTTAACATCGTTCCTGTTTACAATGCCAACGGAAAGTTATAGTAAATATCAAAAGAACTTATCTAACAAACATTTTGTAGTCTTCTTGTTGTTAAAGTAAGAAAGCTTTGTGAATGCATATGTATATATGAATGTATATGTTAATTCAAATTGCGTGTGTGGGCGAAACAAAT

 

---

Search Again