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CRIMIC CR01143

Gene CG5853

CG5853

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2L:9943211..9943229	30F1	FBti0202689	81189

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	short band	GTCTATAACAGCACCACAGTGCCAGT	AACACCGCACTGGGATTATGGAACCA

Images

Construct Design Sequences

sgRNA sequence	ATCATCTGCAGGCTATAACTTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TGCACGAGCTACTGTCCAAG	TCATGAATATCGTCGCTCGTAC	ACTTGGCTGAATTAAACGGCT	TCCCTCACCGTCAAATTGGG

 

Amplicon 5'

Amplicon 3'

TGCACGAGCTACTGTCCAAGCCACTGAAAGAAAGAAAAGCAAGATGTAAACATTCCTCAGTTTGTTAAAAATGGATATATTGGCTCATACGTTGTGGGCTCGTCCAGGAACATCACAGTGGGATTATTGATCAACTCCATGGCAATGGATAGCCTCTTCTTCTGACCACCGGACAGGCGCATTGTAAGGGTCTGATCGTGATTGTACAGACCAAGGAGCAGCAGAATGTCCTCGATCTGCAAGGGTAAATCCGCGATTTATGACTATGATCTTTAAGTGAAGATCACCCCACTCACCCTGCTCTCCTTCTCCTCGTAGCTGACTGTCTGACCCAGTTTAAGATCGGCGGCTATATGCATATTCTCGTTTACCGTCAGCAGCGGCTGCAGACGATCATCCTGGGTGATGTAGCAGGACATCCGACGGAAGGAAGCTGCAGGGAGGGATTACCATGAATTCTGGATATATTGAATAAATTAAGTGAGGTCATATACTAACGCAAATCACGTCGCCGACCATTGAGCAAGATGGAGCCATCCACTCCGGTCGTCTTGAAACCTGAAAGGGCATCTAGCAAAGTGGACTTTCCTGCACCCGAAGGTCCCATGATGGCAATCAGCTGGCTGCCTGGGAACTTTCCGCACACATTGTGCAAGATTTCCTTGGATCCTAAGAAATTGCGAAATTACTTAAATGCCATCGTAAAATATGATCATAAAAACTTAATAGACATTCAGTGTGTTTTTTTTTTATACGGTCGTCGGTATCAAGGTTCAGGGAACCAATGGAGAACCCATGAGTACGCGTACGAGCGACGATATTCATGA

ACTTGGCTGAATTAAACGGCTATTGAGGTAAGATCTCGAGTAAATCTACATTAATCCACTTCAGTGAATAACCTGTTAACTACACAACTCGAAAACAATTCAAATCAGTAAACAATTCTAGGTGATATACGTATCAAGCCATAGAAAAGAGTTGCAAATATAGGGAAAAAGGGTTTAGCTTAATTAACTCAATCTTTCACAGTAATCGTATTGTTATGTTAGCAATATTACACATTTTAGCACTTCTAGTCACCAATTTAACATTCCGATAGCAATGGCCATATATCCACCTACCGAAAGGTTGATTAATTAATTACCGCCACGGGTTGAAAATTTGTGCAGTTCTAATCAAGTTACCAGGGGCTAAACAATGTCGATATCTTTTGAACTTTGAAATTAGCAAAATGTTCTAAAGCCAAACCCATCATATCGAATTACAAATGCTCTACTAACTATCGTATTGGCAAGTTATTTCTTATCATTTTATGGACTCGACGGAAAGGGAATTTATGAATGACTAACTGGCATTGAATATATACAACCGGGAAGGGGGTGTGATCGGAATAAGCGGAGTGATCGGCTCTTATCTAATCAATGGAAAACCAAGTTGGTTAAAGAACTAGCAAGCAAGTGTTTGGCAAGTGATTGGTATGTGGAGGCACTGCCGAAAAGAATAACCTTGTAGTAAACTTTGTTCGGATGTAATTATCTTATGGCACTCGTACTTAAGAAACATTTGAAATATAGTATCTGAAGGATTTGCTTTATCTGAGTATATCTGTATCCCTTAGAATACTATTAATGAAAAGACTTTCTAGCATATGTTTTATCTTCTATTATAACAGTAGTTTTGTCTAGTCATAATTTAGATATTATTTATGAGACTATATTGGTAGGATATAAAGAATTGAAGGTCATTCCCGTTCAAAGAGTAGCCGCTGATCGTAAAACATATATAAATGCCCAGCTAATTTTAAGTTCTCCGCTGCCCCAATCTTATCGAGCCATATCTTAGATCATGCGATCGCAATGGTTAGTTAGCGACAACTCGCTTCTTGTAGCTCACCTCGATTGAAGCCCAATTTGACGGTGAGGGA

 

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