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CRIMIC CR01144

Gene robo3

CG5423

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2L:1264112..1264136	21F3	FBti0202690	81190

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TTTCATTGTCCTTTCCCGGCGAATCG	TCACCTGAATGCGTTGCGTCGTGATA

Images

Construct Design Sequences

sgRNA sequence	GAGGGGTTGACTGGTGTAAAAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GGCACAACTTTCGCTTCGTT	GACAGGCACAAGTATCCCCG	TCTCTTGCACCTTGCTGTCC	TGCGTTAAACTGTTGGCGTG

 

Amplicon 5'

Amplicon 3'

GGCACAACTTTCGCTTCGTTTCTGGGTTTTCGGGTAATAGGCATCCGGTCATAAAAACATGAATTGTGGCTCTTAGCAGCAAATCCAATTGGGCATAAATCGGCTTTGTCGGCAGCACATGAGATGGATCCTTCCTCTCAGATAGCTGCTTTTACCTCCCCGGCTTCCTTTTAGGTTCTTAGGTCCTGGCTCGTTTCATGGCTTTGCTCGGCTCTCTATTGTGGGCTTTATTTTATGTGCATTTAGGCACAATGCGCTTATTGACTTAATGACAAATGTAGTTAGGCAACACAATGACTGACAATGCACTGCGAGAAATGTATCTTTTTCGAGACTATGATAAAAAGGATACTGAGTGCCTTTTATATTGTTGCTTACCTATAGACAGCTTGATTGGCGATTTGGAGTTCATCGTTGTAATATTTAAGATCATTAACTTAAATATCCCTTATTCCGTGAAGTACAAAATATAATCTATTATTATTGTAACACAAATTCTGTTAGTGCTATTTCCCATTCCTGTTCCTGATCCCCTCCCACGTTTCCCGCTGCCGTTAACATTTCTCCTGCTGTTTGTGAGCCAAAGTGCTTGAGTTTCCTATTATAAGAGACTTAACTGAAATTGCATTGTGGCCTGCATCCTGCACTTGTCACCGCCAGCACCCCGAGCTATTTTCTTCTTCATTCTCCGCTGCTTTTCTCAAGGATGTTGACAAATTTACAAACGCGAAAGTGAATTTTCTTTCAATTTTGTTGCAACGACGGGAGCCAGGAGCTGCTATTCCGATCGCCTCTCTCTTTCGCCCTGCCTGCTGGCGTCTCTTTCACTCGAACTTTCCCTCTTCCCCGATTTTTCCTTTACTGCCTGCCTTTCCTGACAGGCAGGATGCGCGCCTAAGTAGTTTTTGTATAATTGCACTCGTCATTGTCGTCGTCGACATTCTTGCCGGGGATACTTGTGCCTGTC

TCTCTTGCACCTTGCTGTCCCTCTCTTTCACTCCTCAGCAGGAGACTAGCTCGCGGCAGTGACAACAACAGCAATTAAGGAATGTTAACAAGCGCACTTTTCAACTTTGGCTCGCGGCTCTCTGTTGCACTTCAAAGGTGTGCCGCTGTGTTTTTCCCTGCGTATGTGTGTGTGTATGTGCCTGTGCCACGCCTATCCGCCTCCTTTCTGCGGCTTTGTCAGGACACAATCTTATGCAGACTCTTCAATTTTGTGACTGATTGGTTGGGAAATCATTCGAATAAGACACCGTGGGATATTTCGAGCTTGCTAGCTGTATAGTAAACCTCATATCAACCTTTGTCCAGTCATATACATCGAGTATTAATCAAAAACATCTCAAATATGGACAGGTCCTTTTAAATCATCATTTAAACCAATTCACTAACCCACTTCAGGTCGGTTAACCTTAGCTTCTGAATTATTCATAGAGCCACATAACCCAATTGCATTCTCAAGCGGACGATGATTGACACATAGCCCACTTAAAGTACCCTGCCAATTAAAAATCAATTTTACAGGACATATCTTGCGTGCCACCGTGTGTCTTCATTTAACAAATCTGAAAATAATTGAAAGTTAGCAGCATAGGTCCCCGAAAACTATGGCATAGTTTTAAAAATTCCCCTGTTGACAATTAAGCTAAGCAGAAAACACAACAAGGACCCTGGGAACCCGAAGCGAAACCAGTCGCAGACACAACAGAAGCAAAACAAAAGCAAACTGAAATGCGTCAAAGGCACGCAACCCTAAACACCGAAAAAGGATAAAGGAATGGGAGGTGAAAGGAGCGTGGGTGGGCTACAGATATATCCACCCACCCATGAGAATGAAGGAAAACTGGTTTCGGTTTCGGTTTCTGCTGGTTAGCAACCCTAATTTACTTTTAATAACTTCATAGGCACAGCTCAAAACAGGCAACGCAGGCAGGGGAGCATTAAAAGTGTCCTTTTTCACATGGCCGAGGAAGAGGACGAATGTGGCGGCGTTAATTTCGATTTTGCTTAACGAGGGTTAAATGCCTGCCCCACGCCAACAGTTTAACGCA

 

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