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CRIMIC CR01145

Gene DIP-theta

CG31646

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2L:5650769..5650773	25F2	FBti0202691	81191

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AAAGTGAATCGCTCGGGCTAGAGCAA	AACGACAACAGAGCCTCAAAGGCCAA

Images

Construct Design Sequences

sgRNA sequence	TGTTGAGCCGCCCTAGCGGAAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CAGCGTGAAAAAGTCGTGCA	TAGGGCGGCTCAACAAGTG	GAAGGAAGGGCAGGACACG	CCGGCTAATTGTTGCCACTG

 

Amplicon 5'

Amplicon 3'

CAGCGTGAAAAAGTCGTGCATACTTTTAGGTGCAGCGCGAAAGCGTGTGAAAAAAGCCAACACGCACAAAAACGAGCACCAATACAAACACAAACACACACACGCGGGCATAGACAACCGGAAAATGGGCGCGAATAAAAGGGAATTGACATAGAAGACACAGCGCGCGGAGGGCGGCAAATTTTTGCAGTCCATGGGAATTGTTGAAAAATTGTCAAACAATCGCTGGCACATCAGCAGCAATTGCTGCTGAGTACAACAAGGAGTGTCTCGACCTCGTGATACCTCGTACTCAGCTTGCAACGAAGTGCCTCTAAAGTTCAAGTGTTTTTCGATAGATATTGTTATATTAATGAAAATGATTAAATGCTCAATTATTTTATACCTATATTGATGATCCCTATTTATTGCATGTTCGCCAAGGACTAGAATATACCCCGTTTGCACTCAAGTAATGGATGAAAACAGTCAAATGGAAATGGTGAAAATGGCTGGGGTAAACGATCGGGAAAACCATGCGAAAATTAAATTTGACTAAAGCGGAACTTGGCCCCGAGATGCAAAGCTAATTGAGGTTTATTGTCGCCAAACCAGAAATACTACAACCTATTCATCATAGCTGGTACTCACTGCAGCATATACTAGTGCATTTAAAGTGGGTAAAATGCTCCCAATTGATTTAAAATCTAGTTGCTATTTGTTATCTCTTTTTGGGTTAATTATATATGTATTCCCAAATTAATCATACTGAAATATCACAGTACATTTTGTGTTTAAATTAGTTTCAAATCAATAATGAACTTTATAAAATAGCTTTTATGTTATGCCAGGCAAACAGGCTATAGTAAACAATGACGTGCATAAAGACTTTCCACATATTTAAAGTTATAAGCCACTTTTAAGCCCGAATGCAAATCCCCATCAGCGCAGGGAATCCCCATCTCAGCTGTAACCCCTTTTCGCAAAATTCATGGGTCACACTGTGGAATAGTTGGTCATCATGTCAAGTCATAAACCCAGAAACCGACGACCCAAAGTGCCATCAATCACAATGTCAATCACTTTTTGTCATAAGTAGACACTGCCACAATCGCAAGCACACACACACATTTGCAAAGGAAGAGAATATATTTTCAGAGGTTCAACATTGTGTGCAATGTCAACGTTTGTCCACTTGTTGAGCCGCCCTA

GAAGGAAGGGCAGGACACGGGATGCGAAGGCGCAGGACTCGACTTGCTGCCCACAGGCCATAGACAAAGAAAACTTAAGTGGTCACTTGTGTGTGCGTGTGTATGTGTGTGTGTGTGTTGGTGGCCTGGGGGGAGGCGCGTGTCGGGTCAGGAGTAAGCCAGAATACGCATACGCCATGTGTGCCCTTAATTATGCTGTGGACTCTGGGCGGACAATGACAACTTAAACAGCCACTAAAGCTAACCAAGCACGTGCTCCTGGAACTCCACTCCCCGCAGCGTTTATCTTCACCATACCAATATGTGCTCAATGAGCCAAGCTGTTTTTTGGGCCCGACCAGACTGGCAGTCCCATAATTCACTTGAAATACCATCCAGATAGCTTTGGCGAGGGTGCCGAGTTTAAGCCACTCAAAACTTTTTGTTACCACTTGGCAAGGGCGTCAAAGTTGACATTGCGACCGGCACTCAGCAGCTAAATCAAAAAAAAAAAACTGGAAATCGTTCAAGCTGTGTTCGATTGCCTTATCCAAGAAATATGCCAGTTCGTGGAATAATTCTGTGGGTTGGCCAAAAGGGAAATCTTCCAATACAAACAAATTAAAAAAATAATTGAATAGCCTGAAGCATTAAGCAAATGGCTTTAAATATATAGATAACAATTTGGTAAAATTTCACAAGCAATTTAATACAACATTTCAATGCTCAACTGTCAAAACTTGAAAATATGCATAAAACTATTGAATATAAATAACTGAACTGAACTGTTGTATTTTTCCAATCTTTAAATGTATTATAACTCCAAGTAAGATTAGTAAACAGATGACAGTTTCTTACCCAGATGATGGGCGATGTGATCGTGCTCCTCGGACTCGCCGATGATGAAGTCCTGGTGCTGGTGGTGCATCTGGTCCAGGTGGTGCAGCTCCCCCTCGTCGTCGCTCTCCTGGGCGGCGCAGCCGGGTAGCGCGAGAGAGAGGCAGATAATCGAGACGACGGCGGCGGTGGCAGTGGCAACAATTAGCCGG

 

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