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CRIMIC CR01146

Gene DIP-kappa

CG31814

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2L:13708522..13708544	34D1	FBti0206882	83252

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TTTAATGGCCAAATCCACGGCATCGG	GAGGGGAAGGAAGGATAAGACCCTAA

Images

Construct Design Sequences

sgRNA sequence	GAAGGTGGTACCATCTCGCGCGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CCAAAAACCGTCTCCCTCGA	ACCTTCATTGCGCAACAGTG	CGTAAATGCACTGAAGGCCG	GGACTTCCACGGCTAGACAC

 

Amplicon 5'

Amplicon 3'

CCAAAAACCGTCTCCCTCGATGACAATCGAATGCTAATGCGGGCAATCAGCAATCGAAAGTTGATCTATGCCGAGGCACAGTATTAATGACCATTCCAATGACAAAAGGACAATGTCGGTCGTCGGCACGCCAAAGTCAAAAGCACCGCATTCCCACAGGACATTCATTCATTCAGTTGGGTAAACATTCAAACGAACCCGATGGCCAACACTTCAGCTCTGAGTGACGGAATGACAGGCTGATGGACAGACACAGACCCCGGGCGCATTAATAGAGTCAATAGAGAGACAATAGTTGAAAATTAACAAATGAATTGAAATTACTTAAACAAACTCATTAGACGCGGGCCGTGTCAGACCGGAGCACCCATCTCCGGGGACTTCCTGCGAACAGTATTAATATTCTGGCACAAATGTGTCGCCATCGCATGTAATTAAATTTTCCAACCTTAATGTGCTAAGCATTGTAATTTTCTCCCCCTCCCCCGACAGCCAGTCACGAGTTTAAATTGTCAGAAACTGTGACAGCCCGACACCTTTTCGCCCAGGTGCAGAGGAAACCGGCGAGGCGACAAGCCGCATATCTATAATTTTCATTAATTTTAATTGGCTTTCGGCGGGGGCGCAACGTCGCTCAATTACAATGTTTTCAAATTTTTATTGAATTTTAAACTTGTACAGGACTTTCTGCTCGCCAAAGGGTTCAACGAAGTATTTCAGGCAATTAAATGAGCATATTTAAATTCATGATTGAATGGTGAATAATAAGAAAGGTTTTATACATCGACTTTAGAAGGATAGACAGTAAATATTTTTAGAAAAAGGATGAATTCCTGTTGCCATTTAAAAACAAATTAGGTTTCTAACAAAGTAGGCTTGAGCTGACTGCCTTAACAGAATGACACAAACCTTTTAAAACCTTCTGTTTGCATACCTTTTGAATATTTTTCGAAAATTTGGGAAGCGTTGGGAAAACGGCGCGACCCCTCAAAATTCGAAATTTTTGTTGTGTTGCTGCCTTTAATTAAAGGTCCGATTAACTATGTCCAAGTTGGGTGCCATCCTGGCGCAAAGTGCTGTTCATCTCAGCGAGCATCGGCAGCTTTTGAGCCAGGCCTTCTTATCATCTATCACCTATCAACGGAGCCGGGTCAAGGTTCGCGGAAACCACTGTTGCGCAATGAAGGT

CGTAAATGCACTGAAGGCCGAGGAGTTCGACGTTCTGATCATCGGAGGAGGAGCCGTGGGTTGTGGTTGTGCTGTAGACGCAGCGTGTCGTGGTCTTAAAACCGCCTTAATTGAGGCAGAGGATTTTGCAAGCGGCTCTAGTTCGCGAACCAGTAAGCTGATCGATGGCAGTGGTTCATATCTGGGCACTGCCCTCCGGGAGAAGGATGTCGAACAGCTGTACATTATGCTGCAAATGATGAGTGAACGGGTGACCATGCTGAAGAACGCTCCGCATCTGAACCGCATCCAGCCCATGATCATACCCATCTACAGTTTTTTGCAGATGCCCTGCACCTGGCTGGGATTGAAGGTAGGTTGACGAGCTAAGACTGGTTGCCACAAGTATGAAATATATATTCCAAGGTTTACGACTGGATATCAGCAGCATCCAATATCCGCGGATCGCACATCATCTCCAGGGAGGCCACCATGTATGAGTTTCCGCTGCTAAAAACCGATGGCCTTCGCGGTGGCGTCGTCTACTATGATGGTCAGGTGGATGATGCCCGGATGTGCGTCGCATTGGTGATGACCGCGGTGGCCCTGGGCGCCAATGTGGGCAACCACATGGAGGTGGTGGAGATTATGCCACAGGAGGGATGCTGCCGAGTTGTTAGCGTTAAGGACAAGATCTCCTGCGAGCAGTTCTACATTCAGTCCAAGGCCGTGATCAATGCCACAGGCTCCAGCACAGATGCCATCCGGCAGATGGACAAGGAGGGAACCGCACCCATTCTATTGCCCACTCTGGAAACCCAAGTATCCCTGCCCCGCTACTTTGGTTCCGGACATTACGGTTTGTTGAGTCCGGCTCTGAAAAATGATGATCTCACTGTATATATGGTGCCGTTTGAGAACCACATGGTTCTAGGCAGTCGCGAAGTGGACCTCGATGAAGTGTCTAGCCGTGGAAGTCC

 

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