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CRIMIC CR01147

Gene Drgx

CG34340

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2L:3676756..3676772	24B1	FBti0202692	81192

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AATCCTTGAGTCCTTGAGTCCTGGCT	TCGCCGTGCACTAAGTAGTTTAGCAG

Images

Construct Design Sequences

sgRNA sequence	AGTTAGGTGCCCATTAGAGGTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
AGAGCTTCGGTGCACTGAAA	GCATCGTGAAGTGGGTGCTA	CCTAACTTTTCCCAGCCCCC	GCCCTTGCTGAAAAACCCAG

 

Amplicon 5'

Amplicon 3'

AGAGCTTCGGTGCACTGAAAGAAATCTATATCAAGTGTTAATAAATCGACTCGTAGTATATGAAAGCGAAAAAGCCAATGTTGAAGCTACTTTGATACTCTCGATTAAATTATTTTCATACCTCATAGACACAGAAACATTTTCAAGTGCCGTACGAAGATGCCAACTTTTCTTTCAGTGCACAATGAGACGAGTTGCTTGTGTGGCAGAGAGGGATGTAGAGATATACATAGATGGTTGGCTGGCTGACTGACTACTGGCTTGTCTGCGCCCCTTTGCGTACTTTTTGAATCAATTTGGCAACAAGGATTTGTTAATCCTGTCTTCCTGACAAGTGCGGTTAGTTGACTTGTTGTTGCTGCCGCTGCTACTGCATGACGTTTGATGTTCGAGCTGGGAAATGGGAAATACGGGATAAATGGGGTGGTGGAAATCCTCCATTGGCTCGGGTTTTGGCCAAAGCTTTCAGCATGAAATTCTTATTGCCCAGTTTTTGGGCAGCTTTGGCTCTGCGCTCTGGGATTTGCACTCGAAACCTAATTGTGCTAATTGCCAGCCGCTTGAACGTCAGTGGAACATGCTCTGGTCTTCTTTTTCGGCCCGAAGCATTTTACGGCCATCCTGGGGCATTTCCATTTGCATTTTAGGCAATTATTGGCTGCAAGGAATTTGATTAGCAAGAAAATTGCAGTGCAGCCGAATGAACTGGTGACGGGCGGAATGGGAAAGGTCGTGATCGTGATTATACAGATGGATATAGGGGAAAAATAAAAGCCATCAACTTAAGATCATTAATTAGTTCCTGAAATTTAGGTAGTAAGTACCTGGTTTCTTATAAATTCATCAATTTTAGATAGAGCCAAGGTTTAGCACCCACTTCACGATGC

CCTAACTTTTCCCAGCCCCCCTTCGAAAATTATGCTGAGTGCCCGGAGCTCAACTCATCGACTATTCGATTAGCCTGCATCGCAATCTATGGCTTTTGCGTACCTCTTTGATTGCTTGATTTAGGCCCGAAATACGTGCTTCATTAGCAGGATATATAGAGCATATCCTGGTGCTTACCTGTGAGCCTGTGATGGCAAAAGAAGCCGAAACATTTTGTAATTATTTTCATAATTAAATATTAATCGATGGCAGGCATAGCGACACTTGTCGAGAAATAGAGATAGAGTAGAACATGACAAACAGCATTAGTGCGCATTACAGAGAGTCTCTAGAGAACTGAGCGCAAGGATATGGGGGTGGTTGTGGGACGACCTTCGGCCTGGCGAAAATGAATGCCAAAGTAACAACATTTCAGCTAATCGGATTAGAGCCCCCAAAAAAGTTAACACACAGGCAGAGAGACACAGAAAGAGATGGAAGGGACCGGAGAGAAAGAGACGGACAGCTTACTCGGCTTTGGCAAGAAGAAAAATAAAATTAAATTTTGCAATTTGCCAGCTAATTATTTATTTAAAATCAAATTGAAAATTAATTTTACAACACGAACGAACAGAAAGTCCGTTGCGTAAAAAGGTCCGACAAAGTTTCCGCCTTTCGACTTTCGACTTTGGTTTTCCATTTTCCGGGGAGTTTGCAAAACCGGGCATTTTGGGGCGGAGGTCAAGTGGGGCTGTCAACGCCTTTCCTTTTCCTCTGTGTCTCTCTCTTTCTGGCTCAAAAGGCAGTTATAAAATATTTCGTAGCATACAAAATGGCGCTGCTGCTAGAAAATTCAATTAGCCTGCAATTTTTGGCAACAGCTGGCTTTCAGCAAAAGCCAAGTGGACAAGGAAAATTCGGACGGGGGGCTGGGTTTTTCAGCAAGGGC

 

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