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CRIMIC CR01148

Gene EcR

CG1765

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2R:6121859..6121887	42A11	FBti0202693	81193

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	ATTCAGAATGGCCACGGCCTCATCAA	AGAGAAAGAGAGTGGCGGTGAGAGTT

Images

Construct Design Sequences

sgRNA sequence	GTCTCTGCACCGGACGAGTGGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
AACAACACCGTGAAATGGCG	GGTCAAGGCTAGTCTGGGC	AAACAAGCCCATGACCCAGG	TGTGCTTCGCAAAGTCGTTG

 

Amplicon 5'

Amplicon 3'

AACAACACCGTGAAATGGCGGCACAAAATCATTGACCTCAATTTTTTCGTCATGCGAACACACACCAACATTCGTATGTATGTGTACTTCGGTACTTGCGGTGCATCGGTGTGCGCGAGTGCAAGTTAAAATAAAAAGCAAAAACAATACAAATAAGAAAAACTTGTCAACGAGCGTTATCGTTCGCCAGCACTGGTATTTTGCTTAAATTATTTGCCATTGTAGGTCCGCTATTCAACTTCATATGCGGTCGTCCTTGAGCATCGGGACTCGCTTTGAACCAATTATGTATACATATGTATGTCATAGGACAGTGCGAATATCGAGCCACCCCTGGTGGGCTTGACAAAAGAAATTCAAATGAGCTGGACGGCATGGAGGCGATGCGAGCATCAGTTGTGCACAGACACGCACCCGAGATTTGCTGCTGCAGCAACAATACCACTTTTTCAGGGGGCAAGGTCATGTACTTGAGCTTTTGCTCTGTACATCACGTTCGACAGAGCGGGACAGCACGCTGCGCGAAAGGGACAGGGCTGACGTCAATGTCCTTGCGGTTTACTTGGCGTCTTTCTGGGTCCCTCTCCCAGTTGCAGTTGTGACCCAGTTTGAATTTTCGGGTGAATCGAACTCGAAGAAATCGTGCGGATTGCACTACAAAGCTCCCTTAGTCACCGACCCAAAGAGAAATATCCGGTTATTGAGCTACACTTTCCTTGAATTGTGTGCAACACTGCTTTGAAATGCGATATGATCAAAGACTGCGAGAAAATCACTTAATATTTGCATTTCCCACAATTGAGATCTGGGACAGTCTGTTATCTTGTTTGCATAGTAATACATTCGGATAACATTGTTTTGTTAATACTTTGCAGAATTATAAATTTAATGACACCGAATGCATATGATGTGAATAGCACTGTTTACTTCTACTCCAGAATTCATACATTTATTCATACATATATATATATCATAGATTTAAAGTTTAAACTTTAATGAATAAGTTCTATTTAATCTAAAAGTATTTAATTATTATTATTATATTGTATTATTTATATTAATATTGTTATTTTATTTGAGTGCCTTTGCATTCACTCGATTCGTACCACGTAGACTTAGATATACAAGCCCAGACTAGCCTTGACC

AAACAAGCCCATGACCCAGGCGCCAGCAAAGACGCCTCGATTGCAGATATTGGCCTTGAACTCCCGCTCGCGTCCTCATTCAAATTGGGTCAAATGCAAATGCTGTCCTGCCGTCGCTGTGGCTGTTTCTGCTTCTGTTTCTGCTCCTGACCTGACCTGGTTTTCCCATTTGGCACGAGGTCAGTGCCACGGCAACGACCAAAGGCGAGCACGAATCGCTCGAGATGACGTCACTGCAGCCCTGCTCAACAATTGCAGTTGCAACACAAACAGGAAGCGAGAGTCGAGAGCCGAAGAACACATCGCAGCACAACTCAAGTAAAGAAGCAGCATTCCTTCAGCTCTCCCTTTTCCGGCTGCACGCTGCGGGGAGGCCCTAAATATAGAACGACTTGAGGTAGGTTCGAACTCAGCAACGAGCGTTGCATCAGCAGCAGCGGCTTCGGAAGGGCACCAAACCGCCCTTATGACTGCACATGGCCCGGTGCAGCATGTAGAATACATGGCTTGCACTCGTCCACGACGCTGCTGATAGTGTACCGAACAACAATGCTGGCTGCCTGTGTGCGTGTTTATTGCACTCTGCAGCGAACGAGCTCTGATGATCCGGGGGAACCGAGCGAAGAACCATTAAATGGGCACCATAAAGTGTGCTCGCACAATGGGAATATAATTAGCCGTGCACCAGAGATGCTGATACCAAAATACCAAAAACGGGAGAGAACTAACGAGCATTTCTTTTTTAAAGTAAGTAGCGACGAACTTCCTTAATTCTGTGATGAAAGCAAAGTTTTTGGATCTTTATTTAAACCGATGTGCAGTTTTCAAGAAACTGAGCGATATAGAATTTTACAAGGTCATTATTTTAAGAAATAGGTATATGGTGAGGATACGAGCTTAAGGAAACCCATTTCGTACAACGACTTTGCGAAGCACA

 

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