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CRIMIC CR01150

Gene Su(var)2-HP2

CG12864

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2R:14616466..14616499	51B6	FBti0202694	81194

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GTGATTTGTCTGTCTACTTTGCGCGC	GAAGATTGATCATTCTGAGCGGGCAC

Images

Construct Design Sequences

sgRNA sequence	CTCAGAAATGGACGCCCCAATGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CTTTGGCGTGGACTCAGACT	CCCTCGTGAACCCTTACCTC	TGCACACTTACCCGCTGATT	ATGCCTTGGAAGCCCTCTTC

 

Amplicon 5'

Amplicon 3'

CTTTGGCGTGGACTCAGACTTATCCAGCGCAACCCTGTCCGGAGCACATTTAAGATCCGTTTCTTGGTGATTTGACGCCATTTCAATATCATCCTGGGGTAAATTGGTAGATCCTGTGAGCTCCTTGGCATTGTCCAAATCGCTAACTAGTTCATCCTGTTTTATTGGCACTTCTAAATTAACCGTCTCCGTTTCCGGATTTTCTTTGCTCGTATCTATTGAGAGAGGAACATTTGTGTTCAAGCTTTCAATTGTATTTTCAGTCTCTGCTGTTTTAGCCTCCTCCTTTGGAATAATTTTGCGAGGCCTTCCGCGTTTTCTTTTGACTGGCTCTCCTTCCCCATCCCGGCAATTAGCACTTTTTTCCTCCATGGTTGCTCCACGGTTCTTACGCTGCTTTTGGCCACGAGGGCGACCTCGCTTTGGTAAGGACTCGCTCTTCGACCGACGCAACTGGGTGCCCAAAGGTTCCGGTTGATCCAGTCTTTCGCTCGAATAGGTTAGCTTTCGCACGTGACCCAATTGCGAGGTGCTAGAATCTGCTGGAATCTTAGATCTGGATAAGCTACGCGACTTTGAGGCAGACCGCTTTTCGAACTCGTAAGATCGCTCCACATCGATCTCCATGATTTGCTCGGCCAGGTCGAGACTCATGCGCTCCACAGCCTCAAGCGTAGCATTATTAGCTACGTCATACAGCTTGCGACTACCGCTAGCCTTGCTGGCCGAGGATTTCGCACTGCTGCTCGCCTCCGGTGGCTCCAAATCCCGCTCCTTTTTATTAGCCTCTTTGTCACTGGCTGCGAAACAAAAAGCAGAAAAAGTTACAAATGTAAGGGTGTAGCAGAGGTAAGGGTTCACGAGGG

TGCACACTTACCCGCTGATTGCTCATTAGCATGCTTAACCTGTTGGGAAGAAGAGGGTTTCTTTTCGTTGGAGCGGGATTTTCTCGCACTATTCTGTTTCTTATAGATTGGCGGCGGCTCGGCGGAACTGAGATTACTCAAATACGACTGCAGGCGTTCGTAGGTGCTGCTCAAAAAAGACTCCTGATCATTCGCATGACGCTGATTCAGCTCACTCTGCGGCTCCTGGCCCTCGTCGTCGGAAACAACAGGTGTCACAGTGTCTACACGGTTTATGTGTGTTTACATATTATGAATATTATCAGAGGGTTGGCAGTATCAACTAGTTAATAACGCTGACTTACCCGCCACCGATGTGGTCCTGCTTGTGTCCGTTGAAATGTCGATCACCGGCTCATCCTCTTCATCGGAGTCTTGATCGCCGTAAAAGTCCGATTCATACTCATAGGACGAATCGCTGTCAGAGTCCCCGTATAAGTCACCCAAATCTATGGAAAATACGAAGTGGTTATGCACAGCCAGATATGTAGCACAATATCGAACTTACTTAATGGATCTGTTTTGGGTTCAAGCCGCGAAGGCCTTAGATCTGTTTTTTGGGGCTTTACAATGGTAACGGTCTTGTTTTTGCCGGAAGCCCATTTATCACCATTTGCAGATACTGCTGTCTTGTTGGCCACAGAATTGGTTTTCTTGTTTTTTGGACATGCATAAGTTTGCCCAGATGACATGGCAATGTTGCTGCTGCTGCTATTGCTAATGCTTCTGTGTGGGGCAGCATCTTGTGACTTTTTGGAACCTGCCTGAGAGGTACTGGGCTTGGGTACGGTCAGCCTGTGCGATGGACGCATTAGGGCTTCTCCTAAGTGTAGGCATAAAGTAAATGTTATTGATAAAATGCGTCATTGAAACCATTGCGAAATGCCCACCTAGGTCGTCCACCGAATCGAACGACTCATACCGTTCGCGCCTCTTGCTCAGCTTTGACTGGCTGCTCTTCCCGCAGAAGAGGGCTTCCAAGGCAT

 

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