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CRIMIC CR01153

Gene ATP8A

CG42321

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2R:13423153..13423174	50A7	FBti0202695	81195

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
short band	OK	AAGTTATACGACCAGTTGGCCACGCA	CAGGTTGGTCTCACCATCCAAATTGG

Images

Construct Design Sequences

sgRNA sequence	GACGAGACTTTCGTTCCCGAGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TCATTATCCGCCCGATGTCG	GGTCGTGGCACCTTCAATTG	CGAAAGTCTCGTCGAAATCG	CTTTTTCGCCGGGCCAAAAT

 

Amplicon 5'

Amplicon 3'

TCATTATCCGCCCGATGTCGTTTCTGCAAATCATGGAAAGATATCAACAACTCAACTGGCTATATGTTACTCTTAGTACTTACAATGTCCTCGATTATCTCCTTAATGGCGCTCACCGAAAGGATGAACATCAGTGGCACTAGCGTGGTGTAACGACCCGTCGGCGACACCTCCGGGATTTGTTGCAGTATGGCAATTAGCAGGAAAAAGCAGTTGGAGTAACGCCGGAATTGCTCGAACAGGAACGCGGGCAAAAAGCTGATGAAACTGCATGCAAATGGGACAGATTGTTGTTTACTTTGTGTCTAATCCATTCATTTAGGTCATGATAGTTATCAGGCGCTAAATGGCTCGTACTCACTCACCTATATTTTGCCGTCGTTATGCGATTATTACCGTACTTAGTGGGCTGTGGCCCATTTAGGTTTATGATACGCCTCTCCCCATCATCTGCATCGTACCCAGCGGACGAGGTGAAGTCCTCGTCATCTGAAAATATGTATGAAAACGTTGATTAATAGGTGATATCAAAACGTGAGAAATCGGAAAATTACACGGCATTTATTACCGTGGACAGCGATGTTTCAATTAAGTTCTCATTTTTTGCTGATTAGTAATCGGCTATTATCTTTTGAATGCTACTATATCAATGATTTGATATTATGGCTATATATACCAAGGACATACAAGTATGAAATTATAGTCGTCCCAAGAACTGTATTATAAGAACAAGACTAATTTAAAACTTCGAGCTTTTATTAATTTTCATTTCCATTTGAAGGTTCGAATTCATGTTCCGAGACGAGGTATGTTGGTGTCCATCGCCATAACCATTCCACCAGATAGAAATTGGCAGCCATCGGATTGCCAAGCGCATACTTCCTTGCAAAATTATGGCAATTGAAGGTGCCACGACC

CGAAAGTCTCGTCGAAATCGAGCTCCAGATACTGCTTGAAGGCCAGGAAGACGATTCGATGGATGTTACTGCCGTGGATGGTCCGCCGGTTGTCGTAGGCCACAAGCGTCTGACCCATGGCGACATCGCAGCCCGGAATATTACCGACCATCCAAATGAGCCACTCCGTATTATTGTCCGGCGGCACGTCCAAGTCGACCATCATCAGCGTGTGATAGTGTTCCGGATCGGCCTTAAACCGAATGATCGGTTGCTTCAGCGTCTCATTAATCACTACCATAATGCCCGGTTTGATGTCGATATCGCAGGGATATAGCACACTGATCACCTTCGTTGGCTTGCAGGCAAAGAGGCGGGGTATCACGTGGTGCCTCTTGAGTTCCGTGACCAGTTTCTCAACTGGACAAAGGATCGGGCAGCTCACCAGCATGGTGGTGGAATTTCAAAATTTTGGTTGACAAAACAGGAAAGAGGAGCTAGGCTTGCTATAATTGGCATATAAGCTTTTTTTATTGTTTATGGAACAACAAGAACCGCAGATGTCTGTTGTTCTAGATCAATTAAAACTGATCAATCGATCAATCGAATAAATAAATTCATTGTTTGGTTTCTATAGTAGTTACTCTAGATCGCATTTCAGTTGTACTATTTTATAGACTGTTGGTCTTCAAAGAATCCCATATTTGTGTACACAATTTCCCCATCATTTGAAAGATTTGAATATTTTTCTAGTCACTTACGACTCCAACGAATGCGCATCGCCTTGTAGACATTACGAAAGTCCATTTTGGCCCGGCGAAAAAG

 

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