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CRIMIC CR01154

Gene Grd

CG7446

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	3L:17830466..17830495	75A3	FBti0202696	81196

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	short	TTAGACCGGCAACAACAGCAGAAGAC	CTTTAATTGTCAGCCGGCTGGAGTAC

Images

Construct Design Sequences

sgRNA sequence	GACGCGGTCGGAGCGCTGATGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
AACTGACCTCATCGCAGCAA	GCCACGGCACATTCTTAATGA	GTTTGGCGCCAGCTGGAG	CCAAATCCGTGCCAACATCG

 

Amplicon 5'

Amplicon 3'

AACTGACCTCATCGCAGCAAGGAGCCTCTCCAGCCACCAAGGACAGCGCTCCAACTTGCGCATATTTAAACTTTTGATTAGCTGCTGCCTCTTAATGTTGTGCATTTACCCAAATGCCTGGCCCTGGTCCATCGGCCCCGGATCGGGACCCTTCTCTGTCTCGGCCGACAGCATCAAGGGCCGCGGCGATACTCACCGCCTGGGTGAGATGGGCACCTCCTTATCGTCATCCCTGCCGTCCTCGTGGCTCACCCAGAGCAATAACCATGCAAATATATCCGAGTTACTGGACAATCTGCTGCGCGGCTATGACAATAGCATACGTCCGGATTTCGGTGGTAAGTAAGCGAAAAGTCAGAAAAAAGATAGGATAAACTATATACTTGTTGAGTTAGTGCGTAGAATGTTATTCTGACAGATCCATAGAAATGTCGATTAATTAAGTTGATTATGTTTACTGGTCAAGAACAGAATTATGATACCTTTAATGACTACAGGTGTGTTATTAGGTAATAACATTACATAAATAAGTTTATTTTTTCATGCTTTCTATGACAACCATGATAAGTATTATTACTGATTTGGAGTACCAAACATCCCCAAATCATTCAGCCTGTTCCAATTCCTGCGTTGTTTACCCTTTTGACTCCTTCCCCATTCCAAGTGTAATCAGCATAGATTCGCAGATTTTCCGCTCAACGGAAGCATTTAATGAAGCGGAAATCGCTGCGAGGCGACACATCACTCACAAACGGCACCAAGGTGATGAATCGTGCCTTGATGATTGCCTCGCAGCTTATTTGGTAAATAGTTAGTGTCAAAACCGACAAATATTTACCAACACACAAACAATTTTATTTTGCTTTTCCAAGCAACCAGAGCTTTTACTTCGAGGACATTGCACAAAACGGATAAGCAATTCGATTCGTTTTCAATCTCGCAAATGGATGAGGCAATAAAAATTTAAATATTTTCGAAATTTAAAATGCCATTCCACTCAATTGGGGAATGGATTTTGGTCAACGTGGCGAAAATTGTTTTCGCTCAACGGCTTAAGACCAAATTACATTGTTTTTAACACTTCATTAAGAATGTGCCGTGGC

GTTTGGCGCCAGCTGGAGAGGATTCAAAGGATACGCGGCAATGCACATCCGCATCCTGCGAGCGCGACGCATGTTTGCCGAGCGAGCTCAGCAGCAAAGTCATTAAAAACTGTTCGCATAGGCAAAAATACAAAAAATTCCTGGGGGCTTCGGGAGGAGGCGAATCTAGGGCCTGAAAAATTGCATATTAGTGCAGAAAAGCTAGATAAAAATGAGTGTCAGCCGGGGATGGTAACAGATTCAGAGGGTTTGGGACTGTCTGCGAAGCGGCTTAAGTGTCATTGTTCGCACAGAGGAGCATTTAACATTTTCATTTGGGGGCTGCGACTCAAATATGAATATTCATGGTTGAACAAAACCGATACGCAGAGTACGGGGATTATATAGTTGGATTGCGTGGCCTTCAGTTGAAGCAAATCCCATTTCCATAACTAAACTAGTCACAGGCTTGAATGGTAAGCTGTGAGGAAGGTAATAATGTCACAATAAGCCTTGCAACTAAAGTATGTAATACGCAATACATTCATATTCAGCATAGGTTAGTTTTAAATATTAAATATTCTAAAAATATACTTGGATTTTCATACAAAAAACTTTGTTTCGTTACAAACTTTCACTTTATTTCAGGACCTCCAGCCACTATTGAAGTGGATATAATGGTTCGCAGCATGGGCCCAATATCAGAGGTCGATATGGTATGAATAGAATATAGAACATAGAAATATTAATAATATTATTAATTAATTAATTACTTACTTAATTACAATTATATTATTTCCTATACAATAGGAATCATTTTAATCGAAATATCTTTATTCAAAATGTAAGATTACAAACATACGTACCTTAATACATAATGATATTTCTTATTATTTGAAATCTTAAGTTAAAGATTCTAAAGATTCTTTTAATTAGATCTGATTTTTCTGTTTTTCTGATAAGAATCCATAGAAACAAGCTGACAACGCAGCATTTCTGCTTCCCAATTCAATTTGCAGTGACATCTCCTGTCTGACTTTCTTGCTTTTCTGCAATTCAGACTCAGCCGATTTTCTTTATTGCAGACCTACTCGATGGACTGTTATTTCCGCCAATCCTGGGTGGATAAACGTCTCGCATTCGAGGGCGCCCAGGACACGCTGGCACTGAGCGTCTCGATGTTGGCACGGATTTGG

 

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