CR01154

CRIMIC CR01154

Gene Grd

CG7446

Information

Donor Location Cytology FBti Stock number
pM37-p13L:17830466..1783049575A3 FBti020269681196

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
OK short TTAGACCGGCAACAACAGCAGAAGAC CTTTAATTGTCAGCCGGCTGGAGTAC

Images

CR01154-1-A-1.jpgCR01154-1-A-2.jpgCR01154-1-A.jpgCR01154-1-n.jpgCR01154-1.jpg

Construct Design Sequences

sgRNA sequence GACGCGGTCGGAGCGCTGATGGG
sgRNA2 sequence
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
AACTGACCTCATCGCAGCAA GCCACGGCACATTCTTAATGA GTTTGGCGCCAGCTGGAG CCAAATCCGTGCCAACATCG
 
Amplicon 5' Amplicon 3'
AACTGACCTCATCGCAGCAAGGAGCCTCTCCAGCCACCAAGGACAGCGCTCCAACTTGCGCATATTTAAACTTTTGATTAGCTGCTGCCTCTTAATGTTGTGCATTTACCCAAATGCCTGGCCCTGGTCCATCGGCCCCGGATCGGGACCCTTCTCTGTCTCGGCCGACAGCATCAAGGGCCGCGGCGATACTCACCGCCTGGGTGAGATGGGCACCTCCTTATCGTCATCCCTGCCGTCCTCGTGGCTCACCCAGAGCAATAACCATGCAAATATATCCGAGTTACTGGACAATCTGCTGCGCGGCTATGACAATAGCATACGTCCGGATTTCGGTGGTAAGTAAGCGAAAAGTCAGAAAAAAGATAGGATAAACTATATACTTGTTGAGTTAGTGCGTAGAATGTTATTCTGACAGATCCATAGAAATGTCGATTAATTAAGTTGATTATGTTTACTGGTCAAGAACAGAATTATGATACCTTTAATGACTACAGGTGTGTTATTAGGTAATAACATTACATAAATAAGTTTATTTTTTCATGCTTTCTATGACAACCATGATAAGTATTATTACTGATTTGGAGTACCAAACATCCCCAAATCATTCAGCCTGTTCCAATTCCTGCGTTGTTTACCCTTTTGACTCCTTCCCCATTCCAAGTGTAATCAGCATAGATTCGCAGATTTTCCGCTCAACGGAAGCATTTAATGAAGCGGAAATCGCTGCGAGGCGACACATCACTCACAAACGGCACCAAGGTGATGAATCGTGCCTTGATGATTGCCTCGCAGCTTATTTGGTAAATAGTTAGTGTCAAAACCGACAAATATTTACCAACACACAAACAATTTTATTTTGCTTTTCCAAGCAACCAGAGCTTTTACTTCGAGGACATTGCACAAAACGGATAAGCAATTCGATTCGTTTTCAATCTCGCAAATGGATGAGGCAATAAAAATTTAAATATTTTCGAAATTTAAAATGCCATTCCACTCAATTGGGGAATGGATTTTGGTCAACGTGGCGAAAATTGTTTTCGCTCAACGGCTTAAGACCAAATTACATTGTTTTTAACACTTCATTAAGAATGTGCCGTGGC GTTTGGCGCCAGCTGGAGAGGATTCAAAGGATACGCGGCAATGCACATCCGCATCCTGCGAGCGCGACGCATGTTTGCCGAGCGAGCTCAGCAGCAAAGTCATTAAAAACTGTTCGCATAGGCAAAAATACAAAAAATTCCTGGGGGCTTCGGGAGGAGGCGAATCTAGGGCCTGAAAAATTGCATATTAGTGCAGAAAAGCTAGATAAAAATGAGTGTCAGCCGGGGATGGTAACAGATTCAGAGGGTTTGGGACTGTCTGCGAAGCGGCTTAAGTGTCATTGTTCGCACAGAGGAGCATTTAACATTTTCATTTGGGGGCTGCGACTCAAATATGAATATTCATGGTTGAACAAAACCGATACGCAGAGTACGGGGATTATATAGTTGGATTGCGTGGCCTTCAGTTGAAGCAAATCCCATTTCCATAACTAAACTAGTCACAGGCTTGAATGGTAAGCTGTGAGGAAGGTAATAATGTCACAATAAGCCTTGCAACTAAAGTATGTAATACGCAATACATTCATATTCAGCATAGGTTAGTTTTAAATATTAAATATTCTAAAAATATACTTGGATTTTCATACAAAAAACTTTGTTTCGTTACAAACTTTCACTTTATTTCAGGACCTCCAGCCACTATTGAAGTGGATATAATGGTTCGCAGCATGGGCCCAATATCAGAGGTCGATATGGTATGAATAGAATATAGAACATAGAAATATTAATAATATTATTAATTAATTAATTACTTACTTAATTACAATTATATTATTTCCTATACAATAGGAATCATTTTAATCGAAATATCTTTATTCAAAATGTAAGATTACAAACATACGTACCTTAATACATAATGATATTTCTTATTATTTGAAATCTTAAGTTAAAGATTCTAAAGATTCTTTTAATTAGATCTGATTTTTCTGTTTTTCTGATAAGAATCCATAGAAACAAGCTGACAACGCAGCATTTCTGCTTCCCAATTCAATTTGCAGTGACATCTCCTGTCTGACTTTCTTGCTTTTCTGCAATTCAGACTCAGCCGATTTTCTTTATTGCAGACCTACTCGATGGACTGTTATTTCCGCCAATCCTGGGTGGATAAACGTCTCGCATTCGAGGGCGCCCAGGACACGCTGGCACTGAGCGTCTCGATGTTGGCACGGATTTGG
 
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