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CRIMIC CR01155

Gene Rh4

CG9668

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	3L:16857230..16857252	73D3	FBti0202697	81197

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GGAAATGGGAAGGGGCAACTGTCTTT	GCTCCGAACTTAAGCCGCAAAATCGA

Images

Construct Design Sequences

sgRNA sequence	ACGCCCTCGTGGATCGAACGCGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GAACCTGAACCTGGCGAGAA	CATTGCCTCCCAGGTGAGC	GTCGCTTCCGCAATTCACAA	CCAGGTGTGTGGACCTTACG

 

Amplicon 5'

Amplicon 3'

GAACCTGAACCTGGCGAGAATTGCAGGGACCCAGAAGAATTGTAGACACATTCGTGCCGTTGCGAGAACCCTTCCAATGTCCAATAAAATCAACCCGAAGCGCCGCGAACCGACAGCAGCAGCAGCAGGAGCCGGTGCCACGGGAGTGGCCACCAACACGAGCACCTCGACCGGATCCTCCAGTGCCGGCAACACGTCCTCGGCCAACACATCCTCGTCGTCCAGCTCCTCGCTGTCGTCCGCCGGTGGCGGTGATGCGGGCATGTCCGCCGACTTGACCTCGCTGTTTGAATGCCCCGTGTGCTTTGACTATGTGCTACCGCCGATCCTGCAGTGCTCCAGCGGGCACCTGGTGTGCGTGTCCTGTCGCTCCAAGCTCACCTGCTGCCCCACCTGCCGCGGTCCGCTGGCGAATATCCGCAACCTGGCGATGGAGAAGGTTGCCTCGAACGTCAAGTTCCCGTGCAAGCACTCCGGCTACGGATGCACCGCCTCGCTGGTTTACACGGAAAAGACAGAACACGAGGAGACGTGCGAGTGCCGGCCCTACCTGTGTCCGTGCCCGGGCGCCAGCTGTAAGTGGCAGGGCCCGCTTGACCTAGTCATGCAGCATCTGATGATGTCGCACAAGAGCATCACAACGCTGCAGGGCGAGGACATCGTGTTCCTGGCCACCGACATCAACCTGCCCGGCGCCGTCGATTGGGTAATGATGCAGTCCTGCTTCGGCCACCATTTTATGCTCGTGCTGGAGAAGCAGGAGAAGTACGACGGACACCAGCAGTTCTTTGCCATCGTTCAGCTGATCGGGTCGCGCAAGGAAGCGGAGAACTTTGTCTATCGCCTGGAGTTGAACGGAAACCGACGCCGGCTCACCTGGGAGGCAATG

GTCGCTTCCGCAATTCACAACTCGGACTGCCTGGTGTTCGACACGTCGATTGCGCAATTGTTCGCCGACAATGGCAACCTGGGCATTAACGTGACCATATCTCTGGTCTAAACGCACTGTCAATTCGTCGGGTTCTTTCATTTCTTGTGTTTACTATTCCGATACAAATACATATATAGTTTATAGCCCTCTGTTCGCATCAGGCGTCTAACTCGTGGACAGATTGTGAGTCTGGAGATTAAGAAGAAAGAGTTTTGCAGAGTTGTTTTATAAAAATGATTCTCTATTCTAAGAGCCACTTACATGCATATGTATATATATCTATGGAAATATCAGGCTGCTTCGATTATCACATTCGGCACGATATCGCAGTTTTCTTTGATTTTTAAGCTAAGACACTCACAATTTTCCAAAGCGAAATGGAAAGCGTATTAAAGCAATTGCTAAGTGATGTTCAGTGTTTATTGCACACGTTCGTTAAACCTGTAAAACCGCGTTTAAAATTCGTATTTTCATTTTTAGTTTGCACATGACTGTGTGTAAATCTCACTCGTTTGCAGAAAAATGCCAAATATTTTTGGTGAGATTTCGCAGATTTGTATAACACACTTTCCTGTCGAATGCGAAAATCGTGGCTGACCTGTATGTTTGTATGTTTAATCGGTTCGATTGGTACTCTAGTTCTAGGACATGATTTTTCAGTTACCGCACACACAATACTATATATTTCAAAGTTTCAGTGTTACCCGTTTTGTTAGTTCATGGGGTTCTGCTTTAGTTTAATTTAGTTCGTAAGGTCCACACACCTGG

 

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