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CRIMIC CR01156

Gene Herc4

CG9153

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	3L:1314416..1314445	61F6	FBti0202698	81198

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK

Images

Construct Design Sequences

sgRNA sequence	ATGATTGCGGGGTTTAGACTCGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TCAAAGCGGATCTTAAAAGTCCG	GCTCGCGTAGTTGGTGAGTA	CAATCATCTTCCGGCCGACT	CCATTCGCGGTAGCATCTCA

 

Amplicon 5'

Amplicon 3'

TCAAAGCGGATCTTAAAAGTCCGCAAGCGATGCAGCCCTAGCTTAAGCCCATTGTCAGGGCTGCCAACGTGGGCAATGTGGCCCTATCCCAACCAGTTGGCATCGCCACCATTATTTATTGGTTTTTTGTTGCTGGTTTTTGAGTTTTTATATTTTTTTTCTAGAAGGCCTAAAATTTATAGAGCGTTGTAGGTAACAGGATTCAGGATTAGACTTAGATTCTGAAGGCACACCCATCGAAAGCTTGACGAGGCATGAACCGAGCAATTGGAATCGGGAGCAAGTGGACACAAAAGAATGTAGCTAACAATTGGGGACATTCTCGAAAATTCTTGTAGCACCAACAAGTAAATTATTATACCCAGAACAACCGAAGGAGCTATTGGGCGGATCCAGAGCAATGGCATCTGGCAAAGAGCTCTATTGCTGGGGAAGCACGTCACATGGTCAATTGGGACTTGGCGGAATCGAGGACGAGCAGGTAGATAGATGACTTTCGGATATAAGCCCACTGATATTACTTTTGTATGCCATTGATTTCCTCACAGATCCTCACACCTAGTCAGATACCCTGGACACCGGACACTGCTGTACAGCAGGTCGCCTGTGGACACCGACACACTCTCTTTCTGACCGCCACTGGAAAAGTCTACGCTTGCGGGAGCAACGACTACTCCCAGCTGGGCCACGATCTGCCCACCAAAAGGCCACGTATGTCGCCATTTCGTATGTAATCTTCATTTATCGTCTTGTCTGTCCACTCTCCACTAACTCCACCTCATCTCCATACTCACCAACTACGCGAGC

CAATCATCTTCCGGCCGACTTTTTGCAGTGCTTATCCCCGAACTGCAGGATTACGTGATCATCCAGATCTGCTGCGGCAGCCGCCACTCATTGGCGCTCTCCGATTGGGGCCAGGTCTTAAGCTGGGGCGACAACGACTGCGGCCAGCTGGGCCATGCCACCGATAAGGAGATCGTGCAGCTGCCCAAGGTTGTGCGGCAACTGGTCACAAAGACGGTGGTGCAGATCGCCTGCGGCAACAATCACAGCTTGGCACTGACCAGTTGTAAGTATGGAGTGAAATTTAATGGATTCCATTCACTAGATACACAGATACACATCAGAACTACTTGAAACAAGATAAGATAATTGTATTTCCCAAGTTTAGGGCATTACTAAACAATTCTTATCTAAAGCTAAACAACGATACCTTCCGACTAACATCATCCTTGACGTTTCTAGGTGGCGAGCTGTATTCATGGGGTTCCAACATCTACGGTCAGCTGGGCGTGAACTCCCCCAATGATTTGACACATTGCAACTATCCCCTCCGACTAACCACTCTGCTGGGAATTCCGCTGGCCGCAATTGCCTGTGGAGGCAATCACTCGTTCCTCATCTCCAAGTCCGGTGCGGTGTTCGGATGGGGCAGAAACAACTGCGGGCAACTGGGACTGAATGACGAAACGAACCGCTCGTATCCCACCCAGCTGAAAACGCTGCGCACCCTGGGCGTGCGATTTGTGGCCTGCGGCGATGAGTTCTCCGTTTTTCTGACCAACGAGGGAGGGGTGTTCACCTGTGGAGCTGGCGCCTATGGGCAGTTGGGTCATGGCTTTAGCTCGAATGAGATGCTACCGCGAATGG

 

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